Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25175 and RR42_RS25180 are separated by 457 nucleotides RR42_RS25180 and RR42_RS25185 are separated by 226 nucleotides
RR42_RS25175: RR42_RS25175 - 3-keto-5-aminohexanoate cleavage protein, at 987,898 to 988,821
_RS25175
RR42_RS25180: RR42_RS25180 - AraC family transcriptional regulator, at 989,279 to 990,148
_RS25180
RR42_RS25185: RR42_RS25185 - epimerase, at 990,375 to 991,253
_RS25185
Position (kb) 989
990
991 Strain fitness (log2 ratio) -1
0
1
2 at 988.348 kb on - strand, within RR42_RS25175 at 988.348 kb on - strand, within RR42_RS25175 at 988.405 kb on + strand, within RR42_RS25175 at 988.406 kb on - strand, within RR42_RS25175 at 988.406 kb on - strand, within RR42_RS25175 at 988.423 kb on + strand, within RR42_RS25175 at 989.100 kb on + strand at 989.129 kb on + strand at 989.293 kb on - strand at 989.319 kb on + strand at 989.568 kb on + strand, within RR42_RS25180 at 989.586 kb on - strand, within RR42_RS25180 at 989.694 kb on + strand, within RR42_RS25180 at 989.727 kb on - strand, within RR42_RS25180 at 989.727 kb on - strand, within RR42_RS25180 at 989.856 kb on - strand, within RR42_RS25180 at 989.856 kb on - strand, within RR42_RS25180 at 989.981 kb on + strand, within RR42_RS25180 at 990.173 kb on + strand at 990.174 kb on - strand at 990.245 kb on + strand at 990.306 kb on - strand at 990.340 kb on + strand at 990.388 kb on - strand at 990.584 kb on - strand, within RR42_RS25185 at 990.587 kb on + strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.715 kb on - strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.846 kb on - strand, within RR42_RS25185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 988,348 - RR42_RS25175 0.49 -0.2 988,348 - RR42_RS25175 0.49 -0.1 988,405 + RR42_RS25175 0.55 +0.2 988,406 - RR42_RS25175 0.55 +0.7 988,406 - RR42_RS25175 0.55 -0.2 988,423 + RR42_RS25175 0.57 -1.1 989,100 + -0.7 989,129 + +0.2 989,293 - +0.4 989,319 + -0.2 989,568 + RR42_RS25180 0.33 -0.9 989,586 - RR42_RS25180 0.35 +0.4 989,694 + RR42_RS25180 0.48 -0.1 989,727 - RR42_RS25180 0.51 +0.0 989,727 - RR42_RS25180 0.51 -0.5 989,856 - RR42_RS25180 0.66 -1.2 989,856 - RR42_RS25180 0.66 -0.2 989,981 + RR42_RS25180 0.81 -1.5 990,173 + +0.3 990,174 - -0.6 990,245 + -0.1 990,306 - +2.0 990,340 + +0.3 990,388 - -1.3 990,584 - RR42_RS25185 0.24 -0.8 990,587 + RR42_RS25185 0.24 +0.2 990,665 - RR42_RS25185 0.33 -1.2 990,665 - RR42_RS25185 0.33 -1.7 990,665 - RR42_RS25185 0.33 -0.2 990,715 - RR42_RS25185 0.39 -0.5 990,845 + RR42_RS25185 0.53 +1.2 990,845 + RR42_RS25185 0.53 +1.0 990,846 - RR42_RS25185 0.54 +0.6
Or see this region's nucleotide sequence