Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23865 and RR42_RS23870 are separated by 83 nucleotides RR42_RS23870 and RR42_RS23875 are separated by 51 nucleotides RR42_RS23875 and RR42_RS23880 are separated by 66 nucleotides RR42_RS23880 and RR42_RS23885 are separated by 343 nucleotides
RR42_RS23865: RR42_RS23865 - hypothetical protein, at 684,111 to 684,407
_RS23865
RR42_RS23870: RR42_RS23870 - hypothetical protein, at 684,491 to 684,784
_RS23870
RR42_RS23875: RR42_RS23875 - hypothetical protein, at 684,836 to 685,132
_RS23875
RR42_RS23880: RR42_RS23880 - hypothetical protein, at 685,199 to 685,399
_RS23880
RR42_RS23885: RR42_RS23885 - hypothetical protein, at 685,743 to 686,480
_RS23885
Position (kb) 685
686 Strain fitness (log2 ratio) -2
-1
0
1 at 684.584 kb on + strand, within RR42_RS23870 at 684.584 kb on + strand, within RR42_RS23870 at 684.585 kb on - strand, within RR42_RS23870 at 684.603 kb on - strand, within RR42_RS23870 at 684.782 kb on + strand at 684.782 kb on + strand at 684.783 kb on - strand at 684.916 kb on - strand, within RR42_RS23875 at 684.941 kb on + strand, within RR42_RS23875 at 684.942 kb on - strand, within RR42_RS23875 at 685.043 kb on + strand, within RR42_RS23875 at 685.061 kb on + strand, within RR42_RS23875 at 685.062 kb on - strand, within RR42_RS23875 at 685.068 kb on - strand, within RR42_RS23875 at 685.068 kb on - strand, within RR42_RS23875 at 685.130 kb on + strand at 685.225 kb on + strand, within RR42_RS23880 at 685.225 kb on + strand, within RR42_RS23880 at 685.226 kb on - strand, within RR42_RS23880 at 685.448 kb on + strand at 685.449 kb on - strand at 685.586 kb on + strand at 685.586 kb on + strand at 685.586 kb on + strand at 685.587 kb on - strand at 685.587 kb on - strand at 685.588 kb on + strand at 685.662 kb on - strand at 685.736 kb on - strand at 685.736 kb on - strand at 685.889 kb on - strand, within RR42_RS23885 at 685.937 kb on - strand, within RR42_RS23885 at 685.937 kb on - strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.169 kb on + strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.170 kb on - strand, within RR42_RS23885 at 686.294 kb on + strand, within RR42_RS23885 at 686.294 kb on + strand, within RR42_RS23885 at 686.295 kb on - strand, within RR42_RS23885 at 686.295 kb on - strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.298 kb on + strand, within RR42_RS23885 at 686.382 kb on + strand, within RR42_RS23885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 684,584 + RR42_RS23870 0.32 -0.1 684,584 + RR42_RS23870 0.32 +1.4 684,585 - RR42_RS23870 0.32 -0.4 684,603 - RR42_RS23870 0.38 -2.0 684,782 + -0.2 684,782 + -0.3 684,783 - -0.5 684,916 - RR42_RS23875 0.27 -1.3 684,941 + RR42_RS23875 0.35 -0.3 684,942 - RR42_RS23875 0.36 -0.7 685,043 + RR42_RS23875 0.70 -0.4 685,061 + RR42_RS23875 0.76 +0.2 685,062 - RR42_RS23875 0.76 +0.8 685,068 - RR42_RS23875 0.78 +0.5 685,068 - RR42_RS23875 0.78 -0.2 685,130 + +0.0 685,225 + RR42_RS23880 0.13 -1.3 685,225 + RR42_RS23880 0.13 +0.0 685,226 - RR42_RS23880 0.13 -0.7 685,448 + +0.6 685,449 - -0.9 685,586 + -0.2 685,586 + -0.4 685,586 + -1.8 685,587 - -0.1 685,587 - -0.7 685,588 + -0.6 685,662 - +0.0 685,736 - -1.2 685,736 - -0.4 685,889 - RR42_RS23885 0.20 -1.9 685,937 - RR42_RS23885 0.26 -0.6 685,937 - RR42_RS23885 0.26 -0.9 686,169 + RR42_RS23885 0.58 -0.3 686,169 + RR42_RS23885 0.58 -0.1 686,169 + RR42_RS23885 0.58 -0.1 686,169 + RR42_RS23885 0.58 +0.5 686,170 - RR42_RS23885 0.58 -0.6 686,170 - RR42_RS23885 0.58 -0.7 686,170 - RR42_RS23885 0.58 +0.7 686,170 - RR42_RS23885 0.58 +0.2 686,170 - RR42_RS23885 0.58 +0.4 686,170 - RR42_RS23885 0.58 +0.9 686,294 + RR42_RS23885 0.75 +0.9 686,294 + RR42_RS23885 0.75 +1.0 686,295 - RR42_RS23885 0.75 +0.4 686,295 - RR42_RS23885 0.75 +0.2 686,298 + RR42_RS23885 0.75 +0.6 686,298 + RR42_RS23885 0.75 +0.3 686,298 + RR42_RS23885 0.75 +0.6 686,298 + RR42_RS23885 0.75 +0.0 686,382 + RR42_RS23885 0.87 -0.5
Or see this region's nucleotide sequence