Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS23770

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS23755 and RR42_RS23760 are separated by 140 nucleotidesRR42_RS23760 and RR42_RS23765 are separated by 4 nucleotidesRR42_RS23765 and RR42_RS23770 are separated by 104 nucleotidesRR42_RS23770 and RR42_RS23775 are separated by 647 nucleotides RR42_RS23755: RR42_RS23755 - phenylacetate--CoA ligase, at 654,283 to 655,608 _RS23755 RR42_RS23760: RR42_RS23760 - tRNA-Asn, at 655,749 to 655,824 _RS23760 RR42_RS23765: RR42_RS23765 - tRNA-Gly, at 655,829 to 655,904 _RS23765 RR42_RS23770: RR42_RS23770 - hypothetical protein, at 656,009 to 656,920 _RS23770 RR42_RS23775: RR42_RS23775 - hypothetical protein, at 657,568 to 658,818 _RS23775 Position (kb) 656 657Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 655.158 kb on + strand, within RR42_RS23755at 655.159 kb on - strand, within RR42_RS23755at 655.363 kb on + strand, within RR42_RS23755at 655.363 kb on + strand, within RR42_RS23755at 655.364 kb on - strand, within RR42_RS23755at 655.392 kb on + strand, within RR42_RS23755at 655.756 kb on + strandat 655.768 kb on + strand, within RR42_RS23760at 655.784 kb on + strand, within RR42_RS23760at 655.784 kb on + strand, within RR42_RS23760at 655.837 kb on - strand, within RR42_RS23765at 655.837 kb on - strand, within RR42_RS23765at 655.849 kb on - strand, within RR42_RS23765at 655.849 kb on - strand, within RR42_RS23765at 655.925 kb on + strandat 655.925 kb on + strandat 655.926 kb on - strandat 655.931 kb on - strandat 656.010 kb on + strandat 656.011 kb on - strandat 656.017 kb on - strandat 656.017 kb on - strandat 656.058 kb on + strandat 656.058 kb on + strandat 656.058 kb on + strandat 656.058 kb on + strandat 656.058 kb on + strandat 656.071 kb on + strandat 656.083 kb on + strandat 656.083 kb on + strandat 656.083 kb on + strandat 656.083 kb on + strandat 656.084 kb on - strandat 656.084 kb on - strandat 656.149 kb on + strand, within RR42_RS23770at 656.149 kb on + strand, within RR42_RS23770at 656.177 kb on + strand, within RR42_RS23770at 656.177 kb on + strand, within RR42_RS23770at 656.178 kb on - strand, within RR42_RS23770at 656.178 kb on - strand, within RR42_RS23770at 656.178 kb on - strand, within RR42_RS23770at 656.188 kb on + strand, within RR42_RS23770at 656.189 kb on - strand, within RR42_RS23770at 656.189 kb on - strand, within RR42_RS23770at 656.189 kb on - strand, within RR42_RS23770at 656.328 kb on + strand, within RR42_RS23770at 656.330 kb on + strand, within RR42_RS23770at 656.331 kb on - strand, within RR42_RS23770at 656.341 kb on - strand, within RR42_RS23770at 656.425 kb on + strand, within RR42_RS23770at 656.425 kb on + strand, within RR42_RS23770at 656.426 kb on - strand, within RR42_RS23770at 656.426 kb on - strand, within RR42_RS23770at 656.426 kb on - strand, within RR42_RS23770at 656.444 kb on + strand, within RR42_RS23770at 656.444 kb on + strand, within RR42_RS23770at 656.445 kb on - strand, within RR42_RS23770at 656.497 kb on - strand, within RR42_RS23770at 656.530 kb on + strand, within RR42_RS23770at 656.531 kb on - strand, within RR42_RS23770at 656.531 kb on - strand, within RR42_RS23770at 656.531 kb on - strand, within RR42_RS23770at 656.573 kb on + strand, within RR42_RS23770at 656.574 kb on - strand, within RR42_RS23770at 656.583 kb on - strand, within RR42_RS23770at 656.720 kb on + strand, within RR42_RS23770at 656.726 kb on - strand, within RR42_RS23770at 656.730 kb on + strand, within RR42_RS23770at 656.730 kb on + strand, within RR42_RS23770at 656.731 kb on - strand, within RR42_RS23770at 656.731 kb on - strand, within RR42_RS23770at 656.731 kb on - strand, within RR42_RS23770at 656.777 kb on - strand, within RR42_RS23770at 656.810 kb on - strand, within RR42_RS23770at 656.836 kb on + strandat 657.046 kb on + strandat 657.047 kb on - strandat 657.083 kb on + strandat 657.180 kb on + strandat 657.180 kb on + strandat 657.256 kb on + strandat 657.325 kb on + strandat 657.325 kb on + strandat 657.326 kb on - strandat 657.365 kb on + strandat 657.404 kb on - strandat 657.537 kb on + strandat 657.537 kb on + strandat 657.538 kb on - strandat 657.540 kb on - strandat 657.540 kb on - strandat 657.573 kb on + strandat 657.574 kb on - strandat 657.574 kb on - strandat 657.574 kb on - strandat 657.685 kb on + strandat 657.685 kb on + strandat 657.685 kb on + strandat 657.685 kb on + strandat 657.686 kb on - strandat 657.686 kb on - strandat 657.686 kb on - strandat 657.686 kb on - strandat 657.788 kb on + strand, within RR42_RS23775at 657.788 kb on + strand, within RR42_RS23775at 657.788 kb on + strand, within RR42_RS23775at 657.789 kb on - strand, within RR42_RS23775at 657.817 kb on + strand, within RR42_RS23775at 657.817 kb on + strand, within RR42_RS23775at 657.817 kb on + strand, within RR42_RS23775at 657.817 kb on + strand, within RR42_RS23775at 657.817 kb on + strand, within RR42_RS23775at 657.817 kb on + strand, within RR42_RS23775at 657.818 kb on - strand, within RR42_RS23775at 657.827 kb on - strand, within RR42_RS23775at 657.827 kb on - strand, within RR42_RS23775at 657.833 kb on + strand, within RR42_RS23775at 657.833 kb on + strand, within RR42_RS23775at 657.834 kb on - strand, within RR42_RS23775at 657.838 kb on - strand, within RR42_RS23775at 657.865 kb on + strand, within RR42_RS23775at 657.866 kb on - strand, within RR42_RS23775at 657.866 kb on - strand, within RR42_RS23775at 657.871 kb on + strand, within RR42_RS23775at 657.871 kb on + strand, within RR42_RS23775at 657.871 kb on + strand, within RR42_RS23775at 657.872 kb on - strand, within RR42_RS23775at 657.872 kb on - strand, within RR42_RS23775at 657.872 kb on - strand, within RR42_RS23775at 657.872 kb on - strand, within RR42_RS23775at 657.872 kb on - strand, within RR42_RS23775at 657.900 kb on - strand, within RR42_RS23775

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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655,158 + RR42_RS23755 0.66 +0.9
655,159 - RR42_RS23755 0.66 +0.0
655,363 + RR42_RS23755 0.81 -1.9
655,363 + RR42_RS23755 0.81 +1.1
655,364 - RR42_RS23755 0.82 -0.3
655,392 + RR42_RS23755 0.84 -0.9
655,756 + -0.1
655,768 + RR42_RS23760 0.25 +0.1
655,784 + RR42_RS23760 0.46 +0.6
655,784 + RR42_RS23760 0.46 -0.5
655,837 - RR42_RS23765 0.11 -1.2
655,837 - RR42_RS23765 0.11 -0.4
655,849 - RR42_RS23765 0.26 -0.4
655,849 - RR42_RS23765 0.26 -0.3
655,925 + +0.5
655,925 + -1.3
655,926 - -0.2
655,931 - -0.3
656,010 + +0.3
656,011 - +1.1
656,017 - -1.1
656,017 - +0.0
656,058 + -2.1
656,058 + +1.6
656,058 + +1.1
656,058 + +0.4
656,058 + +0.2
656,071 + +0.1
656,083 + -1.5
656,083 + -0.7
656,083 + +0.6
656,083 + +0.8
656,084 - +0.5
656,084 - +1.1
656,149 + RR42_RS23770 0.15 -0.2
656,149 + RR42_RS23770 0.15 -0.5
656,177 + RR42_RS23770 0.18 +0.1
656,177 + RR42_RS23770 0.18 -1.3
656,178 - RR42_RS23770 0.19 +0.2
656,178 - RR42_RS23770 0.19 -0.2
656,178 - RR42_RS23770 0.19 -0.8
656,188 + RR42_RS23770 0.20 +0.5
656,189 - RR42_RS23770 0.20 +1.0
656,189 - RR42_RS23770 0.20 +0.2
656,189 - RR42_RS23770 0.20 -1.1
656,328 + RR42_RS23770 0.35 +0.3
656,330 + RR42_RS23770 0.35 +1.0
656,331 - RR42_RS23770 0.35 -0.5
656,341 - RR42_RS23770 0.36 -3.9
656,425 + RR42_RS23770 0.46 +1.2
656,425 + RR42_RS23770 0.46 +0.4
656,426 - RR42_RS23770 0.46 -0.2
656,426 - RR42_RS23770 0.46 +1.8
656,426 - RR42_RS23770 0.46 -0.2
656,444 + RR42_RS23770 0.48 -1.6
656,444 + RR42_RS23770 0.48 -0.1
656,445 - RR42_RS23770 0.48 -0.9
656,497 - RR42_RS23770 0.54 -0.7
656,530 + RR42_RS23770 0.57 +0.9
656,531 - RR42_RS23770 0.57 -0.2
656,531 - RR42_RS23770 0.57 +0.6
656,531 - RR42_RS23770 0.57 +0.5
656,573 + RR42_RS23770 0.62 -0.4
656,574 - RR42_RS23770 0.62 +0.6
656,583 - RR42_RS23770 0.63 +0.5
656,720 + RR42_RS23770 0.78 -0.1
656,726 - RR42_RS23770 0.79 -0.3
656,730 + RR42_RS23770 0.79 +0.4
656,730 + RR42_RS23770 0.79 +1.0
656,731 - RR42_RS23770 0.79 -0.4
656,731 - RR42_RS23770 0.79 -0.1
656,731 - RR42_RS23770 0.79 -0.5
656,777 - RR42_RS23770 0.84 +0.3
656,810 - RR42_RS23770 0.88 +0.2
656,836 + +0.0
657,046 + -0.3
657,047 - -0.7
657,083 + -1.1
657,180 + +0.4
657,180 + -0.6
657,256 + -0.5
657,325 + +0.6
657,325 + -0.2
657,326 - +0.4
657,365 + -1.0
657,404 - -0.4
657,537 + +0.5
657,537 + +0.2
657,538 - +0.3
657,540 - +0.8
657,540 - -0.5
657,573 + -0.3
657,574 - +0.0
657,574 - -0.6
657,574 - +0.2
657,685 + -1.1
657,685 + -0.2
657,685 + -0.5
657,685 + +0.5
657,686 - -2.0
657,686 - +0.0
657,686 - -1.0
657,686 - +0.7
657,788 + RR42_RS23775 0.18 +0.9
657,788 + RR42_RS23775 0.18 -1.0
657,788 + RR42_RS23775 0.18 -0.4
657,789 - RR42_RS23775 0.18 +2.0
657,817 + RR42_RS23775 0.20 -0.4
657,817 + RR42_RS23775 0.20 +0.4
657,817 + RR42_RS23775 0.20 -1.2
657,817 + RR42_RS23775 0.20 +0.2
657,817 + RR42_RS23775 0.20 -0.0
657,817 + RR42_RS23775 0.20 +0.4
657,818 - RR42_RS23775 0.20 +0.5
657,827 - RR42_RS23775 0.21 -2.8
657,827 - RR42_RS23775 0.21 +1.8
657,833 + RR42_RS23775 0.21 -0.4
657,833 + RR42_RS23775 0.21 -0.2
657,834 - RR42_RS23775 0.21 -0.4
657,838 - RR42_RS23775 0.22 +0.3
657,865 + RR42_RS23775 0.24 +0.2
657,866 - RR42_RS23775 0.24 -2.3
657,866 - RR42_RS23775 0.24 -1.0
657,871 + RR42_RS23775 0.24 -0.3
657,871 + RR42_RS23775 0.24 +0.2
657,871 + RR42_RS23775 0.24 +1.0
657,872 - RR42_RS23775 0.24 +0.4
657,872 - RR42_RS23775 0.24 +0.8
657,872 - RR42_RS23775 0.24 -0.4
657,872 - RR42_RS23775 0.24 +0.5
657,872 - RR42_RS23775 0.24 -1.3
657,900 - RR42_RS23775 0.27 -0.2

Or see this region's nucleotide sequence