Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23510 and RR42_RS23515 are separated by 175 nucleotides RR42_RS23515 and RR42_RS23520 are separated by 164 nucleotides RR42_RS23520 and RR42_RS23525 are separated by 20 nucleotides
RR42_RS23510: RR42_RS23510 - LysR family transcriptional regulator, at 600,376 to 601,293
_RS23510
RR42_RS23515: RR42_RS23515 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 601,469 to 604,054
_RS23515
RR42_RS23520: RR42_RS23520 - thioesterase, at 604,219 to 604,692
_RS23520
RR42_RS23525: RR42_RS23525 - glycosyl transferase, at 604,713 to 605,834
_RS23525
Position (kb) 601
602
603
604
605 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 600.679 kb on + strand, within RR42_RS23510 at 600.679 kb on + strand, within RR42_RS23510 at 600.679 kb on + strand, within RR42_RS23510 at 600.680 kb on - strand, within RR42_RS23510 at 600.680 kb on - strand, within RR42_RS23510 at 600.680 kb on - strand, within RR42_RS23510 at 600.979 kb on + strand, within RR42_RS23510 at 600.979 kb on + strand, within RR42_RS23510 at 600.979 kb on + strand, within RR42_RS23510 at 600.980 kb on - strand, within RR42_RS23510 at 600.980 kb on - strand, within RR42_RS23510 at 600.980 kb on - strand, within RR42_RS23510 at 600.980 kb on - strand, within RR42_RS23510 at 601.141 kb on + strand, within RR42_RS23510 at 601.240 kb on + strand at 601.249 kb on + strand at 601.249 kb on + strand at 601.250 kb on - strand at 601.250 kb on - strand at 601.380 kb on - strand at 601.385 kb on - strand at 601.414 kb on - strand at 601.495 kb on - strand at 601.668 kb on - strand at 601.758 kb on - strand, within RR42_RS23515 at 601.758 kb on - strand, within RR42_RS23515 at 602.172 kb on - strand, within RR42_RS23515 at 602.315 kb on + strand, within RR42_RS23515 at 602.315 kb on + strand, within RR42_RS23515 at 602.315 kb on + strand, within RR42_RS23515 at 602.316 kb on - strand, within RR42_RS23515 at 602.316 kb on - strand, within RR42_RS23515 at 602.402 kb on + strand, within RR42_RS23515 at 602.402 kb on + strand, within RR42_RS23515 at 602.403 kb on - strand, within RR42_RS23515 at 602.527 kb on + strand, within RR42_RS23515 at 603.327 kb on - strand, within RR42_RS23515 at 603.841 kb on + strand at 603.879 kb on - strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.944 kb on + strand at 603.945 kb on - strand at 603.989 kb on + strand at 603.989 kb on + strand at 603.989 kb on + strand at 603.995 kb on + strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 603.996 kb on - strand at 604.053 kb on - strand at 604.053 kb on - strand at 604.053 kb on - strand at 604.076 kb on + strand at 604.077 kb on - strand at 604.077 kb on - strand at 604.183 kb on - strand at 604.183 kb on - strand at 604.183 kb on - strand at 604.199 kb on + strand at 604.199 kb on + strand at 604.199 kb on + strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.200 kb on - strand at 604.261 kb on + strand at 604.261 kb on + strand at 604.294 kb on - strand, within RR42_RS23520 at 604.445 kb on - strand, within RR42_RS23520 at 604.511 kb on - strand, within RR42_RS23520 at 604.598 kb on - strand, within RR42_RS23520 at 604.598 kb on - strand, within RR42_RS23520 at 604.690 kb on + strand at 604.691 kb on - strand at 604.714 kb on + strand at 604.714 kb on + strand at 604.714 kb on + strand at 604.714 kb on + strand at 604.715 kb on - strand at 604.715 kb on - strand at 604.724 kb on - strand at 604.727 kb on - strand at 604.727 kb on - strand at 604.727 kb on - strand at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.829 kb on - strand, within RR42_RS23525 at 604.831 kb on + strand, within RR42_RS23525 at 604.832 kb on - strand, within RR42_RS23525 at 604.870 kb on + strand, within RR42_RS23525 at 604.871 kb on - strand, within RR42_RS23525 at 604.871 kb on - strand, within RR42_RS23525 at 604.933 kb on + strand, within RR42_RS23525 at 604.934 kb on - strand, within RR42_RS23525 at 604.934 kb on - strand, within RR42_RS23525 at 604.934 kb on - strand, within RR42_RS23525 at 605.032 kb on + strand, within RR42_RS23525
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 600,679 + RR42_RS23510 0.33 +0.1 600,679 + RR42_RS23510 0.33 -0.9 600,679 + RR42_RS23510 0.33 +0.6 600,680 - RR42_RS23510 0.33 -0.1 600,680 - RR42_RS23510 0.33 -0.7 600,680 - RR42_RS23510 0.33 -0.2 600,979 + RR42_RS23510 0.66 +0.2 600,979 + RR42_RS23510 0.66 +1.3 600,979 + RR42_RS23510 0.66 +0.6 600,980 - RR42_RS23510 0.66 -0.7 600,980 - RR42_RS23510 0.66 +0.1 600,980 - RR42_RS23510 0.66 -0.2 600,980 - RR42_RS23510 0.66 +1.0 601,141 + RR42_RS23510 0.83 +0.5 601,240 + +1.1 601,249 + -0.3 601,249 + -0.2 601,250 - +1.8 601,250 - +0.8 601,380 - -1.6 601,385 - -0.2 601,414 - -2.4 601,495 - -1.2 601,668 - -2.6 601,758 - RR42_RS23515 0.11 -0.5 601,758 - RR42_RS23515 0.11 -3.1 602,172 - RR42_RS23515 0.27 -1.5 602,315 + RR42_RS23515 0.33 -2.7 602,315 + RR42_RS23515 0.33 -1.8 602,315 + RR42_RS23515 0.33 +0.7 602,316 - RR42_RS23515 0.33 -1.6 602,316 - RR42_RS23515 0.33 -2.6 602,402 + RR42_RS23515 0.36 -0.9 602,402 + RR42_RS23515 0.36 -1.5 602,403 - RR42_RS23515 0.36 -0.9 602,527 + RR42_RS23515 0.41 -1.9 603,327 - RR42_RS23515 0.72 -2.5 603,841 + -0.9 603,879 - +0.1 603,944 + -1.5 603,944 + -2.7 603,944 + -1.1 603,944 + -1.9 603,944 + -1.3 603,944 + -1.2 603,945 - -0.8 603,989 + -0.3 603,989 + -0.4 603,989 + +0.1 603,995 + -0.8 603,996 - +0.1 603,996 - -1.4 603,996 - -0.7 603,996 - -2.1 603,996 - -1.3 603,996 - -0.2 604,053 - +0.8 604,053 - -0.3 604,053 - +1.7 604,076 + -0.3 604,077 - -0.7 604,077 - -0.3 604,183 - -0.2 604,183 - +1.0 604,183 - +0.7 604,199 + -0.5 604,199 + +0.8 604,199 + +1.9 604,200 - -1.0 604,200 - -0.2 604,200 - -0.1 604,200 - +0.6 604,200 - -0.6 604,200 - +1.6 604,261 + -0.1 604,261 + -0.2 604,294 - RR42_RS23520 0.16 +0.7 604,445 - RR42_RS23520 0.48 -0.4 604,511 - RR42_RS23520 0.62 -0.9 604,598 - RR42_RS23520 0.80 -0.4 604,598 - RR42_RS23520 0.80 +0.2 604,690 + -0.3 604,691 - -0.1 604,714 + +0.5 604,714 + -0.2 604,714 + -1.2 604,714 + -0.1 604,715 - -2.4 604,715 - -0.3 604,724 - +0.4 604,727 - -1.0 604,727 - -0.5 604,727 - +0.0 604,829 - RR42_RS23525 0.10 -0.9 604,829 - RR42_RS23525 0.10 -0.0 604,829 - RR42_RS23525 0.10 -0.4 604,829 - RR42_RS23525 0.10 +0.4 604,829 - RR42_RS23525 0.10 +0.1 604,829 - RR42_RS23525 0.10 -0.4 604,831 + RR42_RS23525 0.11 +0.1 604,832 - RR42_RS23525 0.11 -2.4 604,870 + RR42_RS23525 0.14 +0.6 604,871 - RR42_RS23525 0.14 +0.7 604,871 - RR42_RS23525 0.14 +0.0 604,933 + RR42_RS23525 0.20 -0.2 604,934 - RR42_RS23525 0.20 -0.5 604,934 - RR42_RS23525 0.20 -0.4 604,934 - RR42_RS23525 0.20 -0.3 605,032 + RR42_RS23525 0.28 +0.2
Or see this region's nucleotide sequence