Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23405 and RR42_RS23410 are separated by 236 nucleotides RR42_RS23410 and RR42_RS23415 are separated by 225 nucleotides RR42_RS23415 and RR42_RS23420 are separated by 54 nucleotides
RR42_RS23405: RR42_RS23405 - hypothetical protein, at 578,619 to 579,512
_RS23405
RR42_RS23410: RR42_RS23410 - hypothetical protein, at 579,749 to 580,648
_RS23410
RR42_RS23415: RR42_RS23415 - 4-carboxymuconolactone decarboxylase, at 580,874 to 581,428
_RS23415
RR42_RS23420: RR42_RS23420 - 3-oxoacyl-ACP reductase, at 581,483 to 582,283
_RS23420
Position (kb) 579
580
581 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 578.750 kb on - strand, within RR42_RS23405 at 578.837 kb on + strand, within RR42_RS23405 at 578.838 kb on - strand, within RR42_RS23405 at 578.876 kb on - strand, within RR42_RS23405 at 578.924 kb on + strand, within RR42_RS23405 at 579.201 kb on + strand, within RR42_RS23405 at 579.202 kb on - strand, within RR42_RS23405 at 579.327 kb on + strand, within RR42_RS23405 at 579.327 kb on + strand, within RR42_RS23405 at 579.328 kb on - strand, within RR42_RS23405 at 579.328 kb on - strand, within RR42_RS23405 at 579.700 kb on + strand at 579.700 kb on + strand at 579.751 kb on - strand at 579.756 kb on - strand at 579.870 kb on - strand, within RR42_RS23410 at 579.947 kb on + strand, within RR42_RS23410 at 579.981 kb on + strand, within RR42_RS23410 at 580.041 kb on + strand, within RR42_RS23410 at 580.041 kb on + strand, within RR42_RS23410 at 580.042 kb on - strand, within RR42_RS23410 at 580.202 kb on + strand, within RR42_RS23410 at 580.202 kb on + strand, within RR42_RS23410 at 580.203 kb on - strand, within RR42_RS23410 at 580.338 kb on + strand, within RR42_RS23410 at 580.338 kb on + strand, within RR42_RS23410 at 580.486 kb on + strand, within RR42_RS23410 at 580.697 kb on - strand at 580.727 kb on + strand at 580.728 kb on - strand at 580.728 kb on - strand at 580.762 kb on + strand at 580.965 kb on + strand, within RR42_RS23415 at 580.965 kb on + strand, within RR42_RS23415 at 580.965 kb on + strand, within RR42_RS23415 at 581.033 kb on + strand, within RR42_RS23415 at 581.034 kb on - strand, within RR42_RS23415 at 581.034 kb on - strand, within RR42_RS23415 at 581.243 kb on - strand, within RR42_RS23415 at 581.282 kb on + strand, within RR42_RS23415 at 581.282 kb on + strand, within RR42_RS23415 at 581.283 kb on - strand, within RR42_RS23415 at 581.322 kb on + strand, within RR42_RS23415 at 581.348 kb on + strand, within RR42_RS23415 at 581.476 kb on - strand at 581.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 578,750 - RR42_RS23405 0.15 +1.6 578,837 + RR42_RS23405 0.24 +1.4 578,838 - RR42_RS23405 0.24 +0.3 578,876 - RR42_RS23405 0.29 +0.8 578,924 + RR42_RS23405 0.34 +0.8 579,201 + RR42_RS23405 0.65 -0.5 579,202 - RR42_RS23405 0.65 +1.5 579,327 + RR42_RS23405 0.79 -0.5 579,327 + RR42_RS23405 0.79 +0.8 579,328 - RR42_RS23405 0.79 -0.6 579,328 - RR42_RS23405 0.79 +0.4 579,700 + -0.3 579,700 + -1.2 579,751 - +0.0 579,756 - +0.2 579,870 - RR42_RS23410 0.13 -2.0 579,947 + RR42_RS23410 0.22 -2.9 579,981 + RR42_RS23410 0.26 -0.7 580,041 + RR42_RS23410 0.32 +0.2 580,041 + RR42_RS23410 0.32 +0.4 580,042 - RR42_RS23410 0.33 -0.3 580,202 + RR42_RS23410 0.50 +0.0 580,202 + RR42_RS23410 0.50 +0.2 580,203 - RR42_RS23410 0.50 -2.5 580,338 + RR42_RS23410 0.65 +0.9 580,338 + RR42_RS23410 0.65 -0.5 580,486 + RR42_RS23410 0.82 -0.3 580,697 - -1.2 580,727 + -0.5 580,728 - +0.5 580,728 - -1.1 580,762 + -0.4 580,965 + RR42_RS23415 0.16 +1.5 580,965 + RR42_RS23415 0.16 -0.4 580,965 + RR42_RS23415 0.16 -2.2 581,033 + RR42_RS23415 0.29 -1.2 581,034 - RR42_RS23415 0.29 -0.2 581,034 - RR42_RS23415 0.29 -1.8 581,243 - RR42_RS23415 0.66 -0.6 581,282 + RR42_RS23415 0.74 +0.9 581,282 + RR42_RS23415 0.74 -1.4 581,283 - RR42_RS23415 0.74 -0.7 581,322 + RR42_RS23415 0.81 -0.1 581,348 + RR42_RS23415 0.85 +0.4 581,476 - +0.2 581,476 - -0.3
Or see this region's nucleotide sequence