Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS23400

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS23395 and RR42_RS23400 are separated by 213 nucleotidesRR42_RS23400 and RR42_RS23405 are separated by 264 nucleotides RR42_RS23395: RR42_RS23395 - LysR family transcriptional regulator, at 576,264 to 577,151 _RS23395 RR42_RS23400: RR42_RS23400 - ABC transporter substrate-binding protein, at 577,365 to 578,354 _RS23400 RR42_RS23405: RR42_RS23405 - hypothetical protein, at 578,619 to 579,512 _RS23405 Position (kb) 577 578 579Strain fitness (log2 ratio) -2 -1 0 1 2at 576.376 kb on - strand, within RR42_RS23395at 576.442 kb on - strand, within RR42_RS23395at 576.488 kb on + strand, within RR42_RS23395at 576.702 kb on - strand, within RR42_RS23395at 576.702 kb on - strand, within RR42_RS23395at 576.789 kb on - strand, within RR42_RS23395at 576.789 kb on - strand, within RR42_RS23395at 576.789 kb on - strand, within RR42_RS23395at 576.816 kb on - strand, within RR42_RS23395at 576.845 kb on - strand, within RR42_RS23395at 576.849 kb on - strand, within RR42_RS23395at 576.879 kb on - strand, within RR42_RS23395at 577.073 kb on + strandat 577.074 kb on - strandat 577.178 kb on - strandat 577.184 kb on - strandat 577.227 kb on + strandat 577.228 kb on - strandat 577.245 kb on + strandat 577.247 kb on + strandat 577.248 kb on - strandat 577.248 kb on - strandat 577.326 kb on - strandat 577.341 kb on - strandat 577.341 kb on - strandat 577.351 kb on - strandat 577.373 kb on - strandat 577.494 kb on + strand, within RR42_RS23400at 577.568 kb on + strand, within RR42_RS23400at 577.569 kb on - strand, within RR42_RS23400at 577.569 kb on - strand, within RR42_RS23400at 577.645 kb on - strand, within RR42_RS23400at 577.645 kb on - strand, within RR42_RS23400at 577.646 kb on + strand, within RR42_RS23400at 577.646 kb on + strand, within RR42_RS23400at 577.646 kb on + strand, within RR42_RS23400at 577.647 kb on - strand, within RR42_RS23400at 577.647 kb on - strand, within RR42_RS23400at 577.647 kb on - strand, within RR42_RS23400at 577.677 kb on + strand, within RR42_RS23400at 577.707 kb on + strand, within RR42_RS23400at 577.708 kb on - strand, within RR42_RS23400at 577.725 kb on + strand, within RR42_RS23400at 577.725 kb on + strand, within RR42_RS23400at 577.726 kb on - strand, within RR42_RS23400at 577.776 kb on - strand, within RR42_RS23400at 577.839 kb on + strand, within RR42_RS23400at 577.839 kb on + strand, within RR42_RS23400at 577.840 kb on - strand, within RR42_RS23400at 577.932 kb on + strand, within RR42_RS23400at 577.933 kb on - strand, within RR42_RS23400at 577.979 kb on - strand, within RR42_RS23400at 577.979 kb on - strand, within RR42_RS23400at 577.979 kb on - strand, within RR42_RS23400at 578.008 kb on + strand, within RR42_RS23400at 578.009 kb on - strand, within RR42_RS23400at 578.010 kb on + strand, within RR42_RS23400at 578.010 kb on + strand, within RR42_RS23400at 578.011 kb on - strand, within RR42_RS23400at 578.011 kb on - strand, within RR42_RS23400at 578.157 kb on - strand, within RR42_RS23400at 578.244 kb on - strand, within RR42_RS23400at 578.613 kb on + strandat 578.614 kb on - strandat 578.661 kb on + strandat 578.750 kb on - strand, within RR42_RS23405at 578.837 kb on + strand, within RR42_RS23405at 578.838 kb on - strand, within RR42_RS23405at 578.876 kb on - strand, within RR42_RS23405at 578.924 kb on + strand, within RR42_RS23405at 579.201 kb on + strand, within RR42_RS23405at 579.202 kb on - strand, within RR42_RS23405at 579.327 kb on + strand, within RR42_RS23405at 579.327 kb on + strand, within RR42_RS23405at 579.328 kb on - strand, within RR42_RS23405at 579.328 kb on - strand, within RR42_RS23405

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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576,376 - RR42_RS23395 0.13 -0.3
576,442 - RR42_RS23395 0.20 -2.1
576,488 + RR42_RS23395 0.25 +0.2
576,702 - RR42_RS23395 0.49 -0.8
576,702 - RR42_RS23395 0.49 -0.5
576,789 - RR42_RS23395 0.59 +0.5
576,789 - RR42_RS23395 0.59 +1.1
576,789 - RR42_RS23395 0.59 +0.7
576,816 - RR42_RS23395 0.62 +0.0
576,845 - RR42_RS23395 0.65 +1.0
576,849 - RR42_RS23395 0.66 +0.3
576,879 - RR42_RS23395 0.69 -1.9
577,073 + -0.8
577,074 - -1.2
577,178 - -0.5
577,184 - +1.2
577,227 + -1.2
577,228 - -1.2
577,245 + +0.0
577,247 + -0.1
577,248 - -0.2
577,248 - +0.0
577,326 - +0.5
577,341 - +0.2
577,341 - +2.0
577,351 - -0.4
577,373 - -0.6
577,494 + RR42_RS23400 0.13 +1.1
577,568 + RR42_RS23400 0.21 +0.1
577,569 - RR42_RS23400 0.21 -0.6
577,569 - RR42_RS23400 0.21 -0.2
577,645 - RR42_RS23400 0.28 +1.1
577,645 - RR42_RS23400 0.28 -1.9
577,646 + RR42_RS23400 0.28 +1.0
577,646 + RR42_RS23400 0.28 -0.5
577,646 + RR42_RS23400 0.28 +0.2
577,647 - RR42_RS23400 0.28 +0.1
577,647 - RR42_RS23400 0.28 +0.4
577,647 - RR42_RS23400 0.28 +0.2
577,677 + RR42_RS23400 0.32 -1.7
577,707 + RR42_RS23400 0.35 +0.2
577,708 - RR42_RS23400 0.35 +0.7
577,725 + RR42_RS23400 0.36 +0.8
577,725 + RR42_RS23400 0.36 -0.9
577,726 - RR42_RS23400 0.36 +1.2
577,776 - RR42_RS23400 0.42 +0.1
577,839 + RR42_RS23400 0.48 +0.8
577,839 + RR42_RS23400 0.48 -0.1
577,840 - RR42_RS23400 0.48 +0.1
577,932 + RR42_RS23400 0.57 -0.7
577,933 - RR42_RS23400 0.57 +1.8
577,979 - RR42_RS23400 0.62 +1.5
577,979 - RR42_RS23400 0.62 -0.5
577,979 - RR42_RS23400 0.62 +0.6
578,008 + RR42_RS23400 0.65 -0.8
578,009 - RR42_RS23400 0.65 -0.3
578,010 + RR42_RS23400 0.65 -0.5
578,010 + RR42_RS23400 0.65 +0.2
578,011 - RR42_RS23400 0.65 -0.2
578,011 - RR42_RS23400 0.65 +0.2
578,157 - RR42_RS23400 0.80 -0.3
578,244 - RR42_RS23400 0.89 +1.6
578,613 + +0.5
578,614 - -0.9
578,661 + +0.1
578,750 - RR42_RS23405 0.15 +1.6
578,837 + RR42_RS23405 0.24 +1.4
578,838 - RR42_RS23405 0.24 +0.3
578,876 - RR42_RS23405 0.29 +0.8
578,924 + RR42_RS23405 0.34 +0.8
579,201 + RR42_RS23405 0.65 -0.5
579,202 - RR42_RS23405 0.65 +1.5
579,327 + RR42_RS23405 0.79 -0.5
579,327 + RR42_RS23405 0.79 +0.8
579,328 - RR42_RS23405 0.79 -0.6
579,328 - RR42_RS23405 0.79 +0.4

Or see this region's nucleotide sequence