Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23325 and RR42_RS23330 are separated by 53 nucleotides RR42_RS23330 and RR42_RS23335 overlap by 9 nucleotides RR42_RS23335 and RR42_RS23340 are separated by 491 nucleotides
RR42_RS23325: RR42_RS23325 - class D beta-lactamase, at 564,454 to 565,239
_RS23325
RR42_RS23330: RR42_RS23330 - class A beta-lactamase, at 565,293 to 566,180
_RS23330
RR42_RS23335: RR42_RS23335 - hypothetical protein, at 566,172 to 566,552
_RS23335
RR42_RS23340: RR42_RS23340 - RNA ligase, at 567,044 to 568,270
_RS23340
Position (kb) 566
567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 565.596 kb on - strand, within RR42_RS23330 at 565.596 kb on - strand, within RR42_RS23330 at 565.695 kb on + strand, within RR42_RS23330 at 565.952 kb on + strand, within RR42_RS23330 at 565.952 kb on + strand, within RR42_RS23330 at 566.029 kb on - strand, within RR42_RS23330 at 566.174 kb on - strand at 566.178 kb on + strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.179 kb on - strand at 566.224 kb on - strand, within RR42_RS23335 at 566.274 kb on - strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.347 kb on + strand, within RR42_RS23335 at 566.348 kb on - strand, within RR42_RS23335 at 566.348 kb on - strand, within RR42_RS23335 at 566.849 kb on + strand at 566.850 kb on - strand at 566.850 kb on - strand at 566.919 kb on - strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.921 kb on + strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.922 kb on - strand at 566.945 kb on + strand at 566.945 kb on + strand at 566.945 kb on + strand at 566.946 kb on - strand at 566.981 kb on + strand at 566.982 kb on - strand at 566.982 kb on - strand at 566.982 kb on - strand at 566.982 kb on - strand at 567.011 kb on + strand at 567.060 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.067 kb on - strand at 567.202 kb on - strand, within RR42_RS23340 at 567.202 kb on - strand, within RR42_RS23340 at 567.497 kb on + strand, within RR42_RS23340 at 567.498 kb on - strand, within RR42_RS23340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 565,596 - RR42_RS23330 0.34 -0.9 565,596 - RR42_RS23330 0.34 +0.5 565,695 + RR42_RS23330 0.45 -0.8 565,952 + RR42_RS23330 0.74 -0.1 565,952 + RR42_RS23330 0.74 +0.2 566,029 - RR42_RS23330 0.83 +1.8 566,174 - -1.3 566,178 + -0.5 566,179 - +0.1 566,179 - -2.1 566,179 - -0.2 566,179 - -0.5 566,179 - +0.4 566,224 - RR42_RS23335 0.14 -1.1 566,274 - RR42_RS23335 0.27 -0.8 566,347 + RR42_RS23335 0.46 +0.1 566,347 + RR42_RS23335 0.46 -0.2 566,347 + RR42_RS23335 0.46 -3.0 566,347 + RR42_RS23335 0.46 +0.2 566,348 - RR42_RS23335 0.46 -0.2 566,348 - RR42_RS23335 0.46 -0.5 566,849 + +0.2 566,850 - -0.3 566,850 - +1.1 566,919 - -0.1 566,921 + -0.8 566,921 + -1.6 566,921 + +0.7 566,921 + -0.4 566,921 + +1.3 566,922 - -1.6 566,922 - -0.1 566,922 - +0.1 566,922 - +1.2 566,945 + -0.5 566,945 + -1.6 566,945 + +0.9 566,946 - +0.1 566,981 + +0.6 566,982 - -0.2 566,982 - +0.8 566,982 - -1.1 566,982 - -0.7 567,011 + -0.2 567,060 - +0.9 567,067 - -1.3 567,067 - +1.1 567,067 - +0.4 567,067 - +0.5 567,202 - RR42_RS23340 0.13 -1.5 567,202 - RR42_RS23340 0.13 +0.1 567,497 + RR42_RS23340 0.37 -0.3 567,498 - RR42_RS23340 0.37 -0.4
Or see this region's nucleotide sequence