Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23135 and RR42_RS23140 are separated by 78 nucleotides RR42_RS23140 and RR42_RS23145 are separated by 143 nucleotides
RR42_RS23135: RR42_RS23135 - trans-2-enoyl-CoA reductase, at 522,969 to 524,165
_RS23135
RR42_RS23140: RR42_RS23140 - peptidylprolyl isomerase, at 524,244 to 524,585
_RS23140
RR42_RS23145: RR42_RS23145 - acyl-CoA transferase, at 524,729 to 525,826
_RS23145
Position (kb) 524
525 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 523.307 kb on - strand, within RR42_RS23135 at 523.307 kb on - strand, within RR42_RS23135 at 523.462 kb on + strand, within RR42_RS23135 at 523.462 kb on + strand, within RR42_RS23135 at 523.462 kb on + strand, within RR42_RS23135 at 523.463 kb on - strand, within RR42_RS23135 at 523.463 kb on - strand, within RR42_RS23135 at 523.463 kb on - strand, within RR42_RS23135 at 523.492 kb on + strand, within RR42_RS23135 at 523.497 kb on - strand, within RR42_RS23135 at 523.724 kb on + strand, within RR42_RS23135 at 523.724 kb on + strand, within RR42_RS23135 at 523.724 kb on + strand, within RR42_RS23135 at 523.855 kb on + strand at 523.900 kb on + strand, within RR42_RS23135 at 523.901 kb on - strand, within RR42_RS23135 at 524.077 kb on + strand at 524.135 kb on + strand at 524.135 kb on + strand at 524.135 kb on + strand at 524.135 kb on + strand at 524.136 kb on - strand at 524.136 kb on - strand at 524.136 kb on - strand at 524.136 kb on - strand at 524.136 kb on - strand at 524.195 kb on + strand at 524.196 kb on - strand at 524.198 kb on + strand at 524.504 kb on - strand, within RR42_RS23140 at 524.504 kb on - strand, within RR42_RS23140 at 524.571 kb on + strand at 524.571 kb on + strand at 524.630 kb on + strand at 524.630 kb on + strand at 524.631 kb on - strand at 524.730 kb on + strand at 524.730 kb on + strand at 524.731 kb on - strand at 524.731 kb on - strand at 525.139 kb on + strand, within RR42_RS23145 at 525.139 kb on + strand at 525.139 kb on + strand, within RR42_RS23145 at 525.140 kb on - strand, within RR42_RS23145 at 525.140 kb on - strand, within RR42_RS23145 at 525.187 kb on - strand, within RR42_RS23145 at 525.394 kb on - strand, within RR42_RS23145 at 525.412 kb on - strand, within RR42_RS23145 at 525.412 kb on - strand, within RR42_RS23145 at 525.412 kb on - strand, within RR42_RS23145 at 525.441 kb on + strand, within RR42_RS23145 at 525.442 kb on - strand, within RR42_RS23145
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 523,307 - RR42_RS23135 0.28 -0.4 523,307 - RR42_RS23135 0.28 -2.1 523,462 + RR42_RS23135 0.41 +1.0 523,462 + RR42_RS23135 0.41 +0.6 523,462 + RR42_RS23135 0.41 -0.2 523,463 - RR42_RS23135 0.41 -1.0 523,463 - RR42_RS23135 0.41 +0.8 523,463 - RR42_RS23135 0.41 -1.2 523,492 + RR42_RS23135 0.44 +0.4 523,497 - RR42_RS23135 0.44 -0.3 523,724 + RR42_RS23135 0.63 -0.1 523,724 + RR42_RS23135 0.63 -3.1 523,724 + RR42_RS23135 0.63 +0.7 523,855 + +0.1 523,900 + RR42_RS23135 0.78 +0.0 523,901 - RR42_RS23135 0.78 -1.2 524,077 + -1.6 524,135 + -0.1 524,135 + +0.4 524,135 + -0.7 524,135 + -0.7 524,136 - +0.4 524,136 - -0.5 524,136 - -0.3 524,136 - -0.4 524,136 - -0.9 524,195 + +0.2 524,196 - +0.2 524,198 + -0.9 524,504 - RR42_RS23140 0.76 -0.4 524,504 - RR42_RS23140 0.76 -1.7 524,571 + -0.4 524,571 + +0.1 524,630 + -0.5 524,630 + +0.9 524,631 - +0.4 524,730 + +0.1 524,730 + -0.9 524,731 - -0.9 524,731 - -0.5 525,139 + RR42_RS23145 0.37 -0.9 525,139 + +0.5 525,139 + RR42_RS23145 0.37 -1.1 525,140 - RR42_RS23145 0.37 +0.0 525,140 - RR42_RS23145 0.37 -1.5 525,187 - RR42_RS23145 0.42 +1.8 525,394 - RR42_RS23145 0.61 +0.1 525,412 - RR42_RS23145 0.62 +0.2 525,412 - RR42_RS23145 0.62 -0.8 525,412 - RR42_RS23145 0.62 +0.5 525,441 + RR42_RS23145 0.65 +0.8 525,442 - RR42_RS23145 0.65 +0.4
Or see this region's nucleotide sequence