Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22340 and RR42_RS22345 are separated by 233 nucleotides RR42_RS22345 and RR42_RS22350 are separated by 150 nucleotides RR42_RS22350 and RR42_RS22355 are separated by 211 nucleotides RR42_RS22355 and RR42_RS22360 are separated by 263 nucleotides
RR42_RS22340: RR42_RS22340 - peptidase, at 352,525 to 353,808
_RS22340
RR42_RS22345: RR42_RS22345 - UDP-N-acetylmuramate--alanine ligase, at 354,042 to 354,395
_RS22345
RR42_RS22350: RR42_RS22350 - transporter, at 354,546 to 354,974
_RS22350
RR42_RS22355: RR42_RS22355 - ModE family transcriptional regulator, at 355,186 to 355,512
_RS22355
RR42_RS22360: RR42_RS22360 - hypothetical protein, at 355,776 to 355,985
_RS22360
Position (kb) 354
355 Strain fitness (log2 ratio) -1
0
1 at 353.599 kb on + strand, within RR42_RS22340 at 353.638 kb on + strand, within RR42_RS22340 at 353.692 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.959 kb on + strand at 353.960 kb on - strand at 353.960 kb on - strand at 353.960 kb on - strand at 354.068 kb on - strand at 354.206 kb on + strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.207 kb on - strand, within RR42_RS22345 at 354.318 kb on + strand, within RR42_RS22345 at 354.319 kb on - strand, within RR42_RS22345 at 354.381 kb on + strand at 354.382 kb on - strand at 354.458 kb on + strand at 354.459 kb on - strand at 354.578 kb on + strand at 355.025 kb on - strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.041 kb on + strand at 355.042 kb on - strand at 355.042 kb on - strand at 355.042 kb on - strand at 355.071 kb on - strand at 355.112 kb on - strand at 355.432 kb on + strand, within RR42_RS22355 at 355.449 kb on - strand, within RR42_RS22355 at 355.524 kb on + strand at 355.525 kb on - strand at 355.643 kb on - strand at 355.643 kb on - strand at 355.807 kb on + strand, within RR42_RS22360 at 355.807 kb on + strand, within RR42_RS22360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 353,599 + RR42_RS22340 0.84 -0.3 353,638 + RR42_RS22340 0.87 +0.6 353,692 + +0.1 353,959 + -0.8 353,959 + -1.5 353,959 + +0.3 353,959 + -0.3 353,960 - -0.2 353,960 - +0.5 353,960 - +0.5 354,068 - -0.6 354,206 + RR42_RS22345 0.46 -0.3 354,207 - RR42_RS22345 0.47 +1.5 354,207 - RR42_RS22345 0.47 +0.6 354,318 + RR42_RS22345 0.78 +1.0 354,319 - RR42_RS22345 0.78 +0.5 354,381 + -0.9 354,382 - +0.4 354,458 + -0.3 354,459 - +1.5 354,578 + -0.6 355,025 - -1.4 355,041 + +0.1 355,041 + -0.6 355,041 + +0.3 355,041 + -0.7 355,042 - -0.0 355,042 - -0.6 355,042 - -0.4 355,071 - -0.3 355,112 - +1.1 355,432 + RR42_RS22355 0.75 -1.4 355,449 - RR42_RS22355 0.80 +0.7 355,524 + -1.0 355,525 - -1.5 355,643 - +0.3 355,643 - +0.4 355,807 + RR42_RS22360 0.15 -1.1 355,807 + RR42_RS22360 0.15 +1.0
Or see this region's nucleotide sequence