Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21695 and RR42_RS21700 are separated by 529 nucleotides RR42_RS21700 and RR42_RS21705 are separated by 284 nucleotides
RR42_RS21695: RR42_RS21695 - DNA-binding protein, at 194,447 to 195,034
_RS21695
RR42_RS21700: RR42_RS21700 - membrane protein, at 195,564 to 197,135
_RS21700
RR42_RS21705: RR42_RS21705 - alcohol dehydrogenase, at 197,420 to 198,385
_RS21705
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 194.607 kb on + strand, within RR42_RS21695 at 194.754 kb on + strand, within RR42_RS21695 at 194.754 kb on + strand, within RR42_RS21695 at 194.755 kb on - strand, within RR42_RS21695 at 195.291 kb on + strand at 195.291 kb on + strand at 195.291 kb on + strand at 195.292 kb on - strand at 195.292 kb on - strand at 195.293 kb on + strand at 195.293 kb on + strand at 195.478 kb on - strand at 196.014 kb on - strand, within RR42_RS21700 at 196.631 kb on - strand, within RR42_RS21700 at 196.631 kb on - strand, within RR42_RS21700 at 196.631 kb on - strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.760 kb on - strand, within RR42_RS21700 at 196.867 kb on + strand, within RR42_RS21700 at 196.868 kb on - strand, within RR42_RS21700 at 196.868 kb on - strand, within RR42_RS21700 at 196.963 kb on + strand, within RR42_RS21700 at 196.963 kb on + strand, within RR42_RS21700 at 196.964 kb on - strand, within RR42_RS21700 at 197.300 kb on + strand at 197.301 kb on - strand at 197.301 kb on - strand at 197.311 kb on - strand at 197.356 kb on + strand at 197.356 kb on + strand at 197.356 kb on + strand at 197.357 kb on - strand at 197.589 kb on - strand, within RR42_RS21705 at 197.738 kb on + strand, within RR42_RS21705 at 197.738 kb on + strand, within RR42_RS21705 at 197.739 kb on - strand, within RR42_RS21705 at 197.741 kb on - strand, within RR42_RS21705 at 197.787 kb on - strand, within RR42_RS21705 at 197.798 kb on + strand, within RR42_RS21705 at 197.799 kb on - strand, within RR42_RS21705 at 197.907 kb on - strand, within RR42_RS21705 at 197.987 kb on + strand, within RR42_RS21705 at 197.987 kb on + strand, within RR42_RS21705 at 197.988 kb on - strand, within RR42_RS21705 at 197.996 kb on + strand, within RR42_RS21705 at 197.996 kb on + strand, within RR42_RS21705 at 197.996 kb on + strand, within RR42_RS21705 at 197.997 kb on - strand, within RR42_RS21705 at 198.065 kb on + strand, within RR42_RS21705 at 198.065 kb on + strand, within RR42_RS21705 at 198.065 kb on + strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.069 kb on - strand, within RR42_RS21705 at 198.101 kb on + strand, within RR42_RS21705 at 198.101 kb on + strand, within RR42_RS21705
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 194,607 + RR42_RS21695 0.27 +0.2 194,754 + RR42_RS21695 0.52 -0.1 194,754 + RR42_RS21695 0.52 -0.1 194,755 - RR42_RS21695 0.52 +0.3 195,291 + +0.9 195,291 + -0.2 195,291 + +1.5 195,292 - +0.3 195,292 - +0.5 195,293 + -0.9 195,293 + +0.4 195,478 - +0.7 196,014 - RR42_RS21700 0.29 +0.9 196,631 - RR42_RS21700 0.68 -0.3 196,631 - RR42_RS21700 0.68 +0.5 196,631 - RR42_RS21700 0.68 -0.6 196,639 + RR42_RS21700 0.68 +0.3 196,639 + RR42_RS21700 0.68 +0.3 196,639 + RR42_RS21700 0.68 -0.1 196,639 + RR42_RS21700 0.68 -0.7 196,639 + RR42_RS21700 0.68 -0.2 196,639 + RR42_RS21700 0.68 -0.7 196,640 - RR42_RS21700 0.68 -0.8 196,640 - RR42_RS21700 0.68 -0.5 196,640 - RR42_RS21700 0.68 -1.5 196,640 - RR42_RS21700 0.68 -0.7 196,760 - RR42_RS21700 0.76 -2.8 196,867 + RR42_RS21700 0.83 -0.4 196,868 - RR42_RS21700 0.83 -1.0 196,868 - RR42_RS21700 0.83 +0.0 196,963 + RR42_RS21700 0.89 -0.2 196,963 + RR42_RS21700 0.89 -1.1 196,964 - RR42_RS21700 0.89 +0.5 197,300 + -0.3 197,301 - +0.5 197,301 - -1.5 197,311 - +0.8 197,356 + +0.7 197,356 + +1.7 197,356 + -0.3 197,357 - +0.8 197,589 - RR42_RS21705 0.17 +1.0 197,738 + RR42_RS21705 0.33 -0.3 197,738 + RR42_RS21705 0.33 -0.3 197,739 - RR42_RS21705 0.33 -1.2 197,741 - RR42_RS21705 0.33 +0.2 197,787 - RR42_RS21705 0.38 -0.8 197,798 + RR42_RS21705 0.39 +0.3 197,799 - RR42_RS21705 0.39 +0.4 197,907 - RR42_RS21705 0.50 +0.6 197,987 + RR42_RS21705 0.59 -0.1 197,987 + RR42_RS21705 0.59 -0.3 197,988 - RR42_RS21705 0.59 +0.5 197,996 + RR42_RS21705 0.60 -1.2 197,996 + RR42_RS21705 0.60 -0.9 197,996 + RR42_RS21705 0.60 -1.3 197,997 - RR42_RS21705 0.60 +0.5 198,065 + RR42_RS21705 0.67 +0.5 198,065 + RR42_RS21705 0.67 +0.5 198,065 + RR42_RS21705 0.67 +0.0 198,066 - RR42_RS21705 0.67 -0.1 198,066 - RR42_RS21705 0.67 +0.3 198,066 - RR42_RS21705 0.67 +0.3 198,066 - RR42_RS21705 0.67 +1.1 198,069 - RR42_RS21705 0.67 -0.3 198,101 + RR42_RS21705 0.70 +0.5 198,101 + RR42_RS21705 0.70 +0.5
Or see this region's nucleotide sequence