Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21005 and RR42_RS21010 are separated by 46 nucleotides RR42_RS21010 and RR42_RS21015 are separated by 94 nucleotides
RR42_RS21005: RR42_RS21005 - GDP-L-fucose synthase, at 40,805 to 41,797
_RS21005
RR42_RS21010: RR42_RS21010 - GDP-mannose 4,6-dehydratase, at 41,844 to 42,956
_RS21010
RR42_RS21015: RR42_RS21015 - mannose-1-phosphate guanyltransferase, at 43,051 to 44,475
_RS21015
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 41.097 kb on + strand, within RR42_RS21005 at 41.274 kb on + strand, within RR42_RS21005 at 41.274 kb on + strand, within RR42_RS21005 at 41.343 kb on + strand, within RR42_RS21005 at 41.386 kb on - strand, within RR42_RS21005 at 41.477 kb on + strand, within RR42_RS21005 at 41.478 kb on - strand, within RR42_RS21005 at 41.613 kb on + strand, within RR42_RS21005 at 41.613 kb on + strand, within RR42_RS21005 at 41.614 kb on - strand, within RR42_RS21005 at 42.226 kb on + strand, within RR42_RS21010 at 42.227 kb on - strand, within RR42_RS21010 at 42.227 kb on - strand, within RR42_RS21010 at 42.322 kb on + strand, within RR42_RS21010 at 42.322 kb on + strand, within RR42_RS21010 at 42.439 kb on + strand, within RR42_RS21010 at 42.439 kb on + strand, within RR42_RS21010 at 42.439 kb on + strand, within RR42_RS21010 at 42.439 kb on + strand, within RR42_RS21010 at 42.440 kb on - strand, within RR42_RS21010 at 42.440 kb on - strand, within RR42_RS21010 at 42.460 kb on + strand, within RR42_RS21010 at 42.485 kb on - strand, within RR42_RS21010 at 42.502 kb on + strand, within RR42_RS21010 at 42.502 kb on + strand at 42.509 kb on + strand, within RR42_RS21010 at 42.586 kb on + strand, within RR42_RS21010 at 42.586 kb on + strand, within RR42_RS21010 at 42.670 kb on + strand, within RR42_RS21010 at 42.671 kb on - strand, within RR42_RS21010 at 42.830 kb on - strand, within RR42_RS21010 at 42.922 kb on + strand at 42.923 kb on - strand at 42.941 kb on + strand at 43.064 kb on + strand at 43.064 kb on + strand at 43.068 kb on + strand at 43.069 kb on - strand at 43.104 kb on - strand at 43.104 kb on - strand at 43.104 kb on - strand at 43.184 kb on + strand at 43.184 kb on + strand at 43.184 kb on + strand at 43.184 kb on + strand at 43.184 kb on + strand at 43.185 kb on - strand at 43.185 kb on - strand at 43.185 kb on - strand at 43.185 kb on - strand at 43.211 kb on + strand, within RR42_RS21015 at 43.334 kb on + strand, within RR42_RS21015 at 43.382 kb on - strand, within RR42_RS21015 at 43.382 kb on - strand, within RR42_RS21015 at 43.382 kb on - strand, within RR42_RS21015 at 43.712 kb on + strand, within RR42_RS21015 at 43.850 kb on + strand, within RR42_RS21015 at 43.851 kb on - strand, within RR42_RS21015
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 41,097 + RR42_RS21005 0.29 +0.7 41,274 + RR42_RS21005 0.47 -0.5 41,274 + RR42_RS21005 0.47 +0.7 41,343 + RR42_RS21005 0.54 +0.2 41,386 - RR42_RS21005 0.59 +0.4 41,477 + RR42_RS21005 0.68 +0.2 41,478 - RR42_RS21005 0.68 +1.0 41,613 + RR42_RS21005 0.81 -0.6 41,613 + RR42_RS21005 0.81 -0.9 41,614 - RR42_RS21005 0.81 -0.2 42,226 + RR42_RS21010 0.34 -0.1 42,227 - RR42_RS21010 0.34 -1.5 42,227 - RR42_RS21010 0.34 -1.4 42,322 + RR42_RS21010 0.43 +0.2 42,322 + RR42_RS21010 0.43 -1.5 42,439 + RR42_RS21010 0.53 +0.7 42,439 + RR42_RS21010 0.53 -0.1 42,439 + RR42_RS21010 0.53 -0.3 42,439 + RR42_RS21010 0.53 +1.1 42,440 - RR42_RS21010 0.54 -0.3 42,440 - RR42_RS21010 0.54 -1.7 42,460 + RR42_RS21010 0.55 +0.1 42,485 - RR42_RS21010 0.58 +0.3 42,502 + RR42_RS21010 0.59 -1.7 42,502 + +1.1 42,509 + RR42_RS21010 0.60 -1.2 42,586 + RR42_RS21010 0.67 -0.4 42,586 + RR42_RS21010 0.67 +3.7 42,670 + RR42_RS21010 0.74 -0.1 42,671 - RR42_RS21010 0.74 -1.2 42,830 - RR42_RS21010 0.89 -0.9 42,922 + +0.0 42,923 - -0.1 42,941 + -0.0 43,064 + +0.9 43,064 + +0.1 43,068 + -1.2 43,069 - -1.0 43,104 - -0.5 43,104 - +0.1 43,104 - -1.2 43,184 + +0.5 43,184 + -0.2 43,184 + -0.1 43,184 + +0.1 43,184 + -0.2 43,185 - +0.5 43,185 - -0.3 43,185 - -0.2 43,185 - -0.1 43,211 + RR42_RS21015 0.11 -1.7 43,334 + RR42_RS21015 0.20 -0.1 43,382 - RR42_RS21015 0.23 -1.0 43,382 - RR42_RS21015 0.23 +0.1 43,382 - RR42_RS21015 0.23 -3.1 43,712 + RR42_RS21015 0.46 +0.9 43,850 + RR42_RS21015 0.56 +0.3 43,851 - RR42_RS21015 0.56 -0.3
Or see this region's nucleotide sequence