Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20520 and RR42_RS20525 are separated by 17 nucleotides RR42_RS20525 and RR42_RS20530 are separated by 70 nucleotides RR42_RS20530 and RR42_RS20535 are separated by 568 nucleotides
RR42_RS20520: RR42_RS20520 - histidine kinase, at 4,446,404 to 4,447,810
_RS20520
RR42_RS20525: RR42_RS20525 - LuxR family transcriptional regulator, at 4,447,828 to 4,448,496
_RS20525
RR42_RS20530: RR42_RS20530 - phosphoenolpyruvate carboxykinase, at 4,448,567 to 4,450,423
_RS20530
RR42_RS20535: RR42_RS20535 - 3-phosphoglycerate dehydrogenase, at 4,450,992 to 4,452,005
_RS20535
Position (kb)
4448
4449
4450
4451 Strain fitness (log2 ratio)
-1
0
1
2 at 4447.706 kb on - strand at 4448.116 kb on + strand, within RR42_RS20525 at 4448.117 kb on - strand, within RR42_RS20525 at 4448.117 kb on - strand, within RR42_RS20525 at 4448.117 kb on - strand, within RR42_RS20525 at 4448.117 kb on - strand, within RR42_RS20525 at 4448.117 kb on - strand, within RR42_RS20525 at 4448.117 kb on - strand, within RR42_RS20525 at 4448.153 kb on - strand, within RR42_RS20525 at 4448.224 kb on + strand, within RR42_RS20525 at 4448.224 kb on + strand, within RR42_RS20525 at 4448.224 kb on + strand, within RR42_RS20525 at 4448.224 kb on + strand, within RR42_RS20525 at 4448.225 kb on - strand, within RR42_RS20525 at 4448.225 kb on - strand, within RR42_RS20525 at 4448.225 kb on - strand, within RR42_RS20525 at 4448.564 kb on + strand at 4448.564 kb on + strand at 4448.565 kb on - strand at 4448.565 kb on - strand at 4448.568 kb on + strand at 4448.569 kb on - strand at 4448.901 kb on + strand, within RR42_RS20530 at 4448.901 kb on + strand, within RR42_RS20530 at 4448.901 kb on + strand, within RR42_RS20530 at 4448.902 kb on - strand, within RR42_RS20530 at 4448.902 kb on - strand, within RR42_RS20530 at 4448.902 kb on - strand, within RR42_RS20530 at 4448.902 kb on - strand, within RR42_RS20530 at 4448.967 kb on + strand, within RR42_RS20530 at 4448.967 kb on + strand, within RR42_RS20530 at 4448.968 kb on - strand, within RR42_RS20530 at 4448.968 kb on - strand, within RR42_RS20530 at 4449.081 kb on + strand, within RR42_RS20530 at 4449.081 kb on + strand, within RR42_RS20530 at 4449.081 kb on + strand, within RR42_RS20530 at 4449.082 kb on - strand, within RR42_RS20530 at 4449.082 kb on - strand, within RR42_RS20530 at 4449.082 kb on - strand, within RR42_RS20530 at 4449.082 kb on - strand, within RR42_RS20530 at 4449.082 kb on - strand, within RR42_RS20530 at 4449.082 kb on - strand, within RR42_RS20530 at 4449.190 kb on + strand, within RR42_RS20530 at 4449.190 kb on + strand, within RR42_RS20530 at 4449.191 kb on - strand, within RR42_RS20530 at 4449.404 kb on - strand, within RR42_RS20530 at 4449.624 kb on + strand, within RR42_RS20530 at 4449.624 kb on + strand, within RR42_RS20530 at 4449.624 kb on + strand, within RR42_RS20530 at 4449.624 kb on + strand, within RR42_RS20530 at 4449.765 kb on + strand, within RR42_RS20530 at 4449.765 kb on + strand, within RR42_RS20530 at 4449.765 kb on + strand, within RR42_RS20530 at 4449.766 kb on - strand, within RR42_RS20530 at 4449.766 kb on - strand, within RR42_RS20530 at 4449.804 kb on + strand, within RR42_RS20530 at 4449.804 kb on + strand, within RR42_RS20530 at 4449.804 kb on + strand, within RR42_RS20530 at 4449.805 kb on - strand, within RR42_RS20530 at 4449.805 kb on - strand, within RR42_RS20530 at 4449.805 kb on - strand, within RR42_RS20530 at 4449.805 kb on - strand, within RR42_RS20530 at 4449.946 kb on + strand, within RR42_RS20530 at 4449.946 kb on + strand, within RR42_RS20530 at 4449.946 kb on + strand, within RR42_RS20530 at 4449.946 kb on + strand, within RR42_RS20530 at 4449.946 kb on + strand, within RR42_RS20530 at 4449.947 kb on - strand, within RR42_RS20530 at 4449.947 kb on - strand, within RR42_RS20530 at 4449.961 kb on - strand, within RR42_RS20530 at 4449.961 kb on - strand, within RR42_RS20530 at 4449.961 kb on - strand, within RR42_RS20530 at 4450.035 kb on + strand, within RR42_RS20530 at 4450.036 kb on - strand, within RR42_RS20530 at 4450.036 kb on - strand, within RR42_RS20530 at 4450.036 kb on - strand, within RR42_RS20530 at 4450.036 kb on - strand, within RR42_RS20530 at 4450.036 kb on - strand, within RR42_RS20530 at 4450.036 kb on - strand, within RR42_RS20530 at 4450.036 kb on - strand, within RR42_RS20530 at 4450.278 kb on + strand at 4450.278 kb on + strand at 4450.278 kb on + strand at 4450.278 kb on + strand at 4450.279 kb on - strand at 4450.279 kb on - strand at 4450.305 kb on + strand at 4450.305 kb on + strand at 4450.305 kb on + strand at 4450.305 kb on + strand at 4450.306 kb on - strand at 4450.306 kb on - strand at 4450.306 kb on - strand at 4450.306 kb on - strand at 4450.306 kb on - strand at 4450.306 kb on - strand at 4450.306 kb on - strand at 4450.306 kb on - strand at 4450.309 kb on + strand at 4450.309 kb on + strand at 4450.309 kb on + strand at 4450.309 kb on + strand at 4450.309 kb on + strand at 4450.309 kb on + strand at 4450.310 kb on - strand at 4450.310 kb on - strand at 4450.310 kb on - strand at 4450.310 kb on - strand at 4450.310 kb on - strand at 4450.383 kb on - strand at 4450.423 kb on + strand at 4450.423 kb on + strand at 4450.423 kb on + strand at 4450.423 kb on + strand at 4450.424 kb on - strand at 4450.424 kb on - strand at 4450.424 kb on - strand at 4450.424 kb on - strand at 4450.429 kb on + strand at 4450.429 kb on + strand at 4450.430 kb on - strand at 4450.430 kb on - strand at 4450.430 kb on - strand at 4450.442 kb on - strand at 4450.587 kb on + strand at 4450.587 kb on + strand at 4450.756 kb on + strand at 4450.757 kb on - strand at 4450.824 kb on - strand at 4450.824 kb on - strand at 4450.919 kb on + strand at 4450.919 kb on + strand at 4450.920 kb on - strand at 4450.920 kb on - strand at 4450.920 kb on - strand at 4450.925 kb on + strand at 4450.926 kb on - strand at 4450.926 kb on - strand at 4450.926 kb on - strand at 4450.927 kb on + strand at 4450.981 kb on + strand at 4450.981 kb on + strand at 4451.006 kb on - strand at 4451.016 kb on + strand at 4451.017 kb on - strand at 4451.148 kb on - strand, within RR42_RS20535 at 4451.255 kb on + strand, within RR42_RS20535 at 4451.256 kb on - strand, within RR42_RS20535 at 4451.256 kb on - strand, within RR42_RS20535 at 4451.358 kb on + strand, within RR42_RS20535 at 4451.358 kb on + strand, within RR42_RS20535 at 4451.359 kb on - strand, within RR42_RS20535 at 4451.359 kb on - strand, within RR42_RS20535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 4,447,706 - -0.3 4,448,116 + RR42_RS20525 0.43 -0.4 4,448,117 - RR42_RS20525 0.43 -0.5 4,448,117 - RR42_RS20525 0.43 +0.1 4,448,117 - RR42_RS20525 0.43 +0.4 4,448,117 - RR42_RS20525 0.43 -0.1 4,448,117 - RR42_RS20525 0.43 -0.9 4,448,117 - RR42_RS20525 0.43 -0.6 4,448,153 - RR42_RS20525 0.49 -1.2 4,448,224 + RR42_RS20525 0.59 +0.2 4,448,224 + RR42_RS20525 0.59 +0.1 4,448,224 + RR42_RS20525 0.59 -1.1 4,448,224 + RR42_RS20525 0.59 -0.7 4,448,225 - RR42_RS20525 0.59 -0.3 4,448,225 - RR42_RS20525 0.59 -1.6 4,448,225 - RR42_RS20525 0.59 -0.9 4,448,564 + -0.1 4,448,564 + +0.1 4,448,565 - -1.6 4,448,565 - +0.4 4,448,568 + -0.0 4,448,569 - -1.4 4,448,901 + RR42_RS20530 0.18 -0.8 4,448,901 + RR42_RS20530 0.18 +0.5 4,448,901 + RR42_RS20530 0.18 -0.1 4,448,902 - RR42_RS20530 0.18 +0.1 4,448,902 - RR42_RS20530 0.18 -0.8 4,448,902 - RR42_RS20530 0.18 +0.1 4,448,902 - RR42_RS20530 0.18 -0.6 4,448,967 + RR42_RS20530 0.22 -0.3 4,448,967 + RR42_RS20530 0.22 +1.1 4,448,968 - RR42_RS20530 0.22 +0.2 4,448,968 - RR42_RS20530 0.22 -0.1 4,449,081 + RR42_RS20530 0.28 -1.5 4,449,081 + RR42_RS20530 0.28 +1.4 4,449,081 + RR42_RS20530 0.28 -0.6 4,449,082 - RR42_RS20530 0.28 +0.9 4,449,082 - RR42_RS20530 0.28 -0.0 4,449,082 - RR42_RS20530 0.28 -0.0 4,449,082 - RR42_RS20530 0.28 -1.3 4,449,082 - RR42_RS20530 0.28 +0.7 4,449,082 - RR42_RS20530 0.28 -0.1 4,449,190 + RR42_RS20530 0.34 -0.1 4,449,190 + RR42_RS20530 0.34 +0.3 4,449,191 - RR42_RS20530 0.34 -0.4 4,449,404 - RR42_RS20530 0.45 -0.0 4,449,624 + RR42_RS20530 0.57 -1.3 4,449,624 + RR42_RS20530 0.57 +0.8 4,449,624 + RR42_RS20530 0.57 +0.3 4,449,624 + RR42_RS20530 0.57 -0.7 4,449,765 + RR42_RS20530 0.65 -0.5 4,449,765 + RR42_RS20530 0.65 +0.2 4,449,765 + RR42_RS20530 0.65 +0.1 4,449,766 - RR42_RS20530 0.65 -0.4 4,449,766 - RR42_RS20530 0.65 +0.5 4,449,804 + RR42_RS20530 0.67 +1.1 4,449,804 + RR42_RS20530 0.67 +1.4 4,449,804 + RR42_RS20530 0.67 -0.6 4,449,805 - RR42_RS20530 0.67 +0.2 4,449,805 - RR42_RS20530 0.67 -0.6 4,449,805 - RR42_RS20530 0.67 -0.4 4,449,805 - RR42_RS20530 0.67 +1.9 4,449,946 + RR42_RS20530 0.74 +0.2 4,449,946 + RR42_RS20530 0.74 -0.9 4,449,946 + RR42_RS20530 0.74 +0.8 4,449,946 + RR42_RS20530 0.74 -0.2 4,449,946 + RR42_RS20530 0.74 -1.7 4,449,947 - RR42_RS20530 0.74 -0.9 4,449,947 - RR42_RS20530 0.74 -0.2 4,449,961 - RR42_RS20530 0.75 +1.0 4,449,961 - RR42_RS20530 0.75 -0.5 4,449,961 - RR42_RS20530 0.75 -0.2 4,450,035 + RR42_RS20530 0.79 +0.7 4,450,036 - RR42_RS20530 0.79 +0.8 4,450,036 - RR42_RS20530 0.79 +0.1 4,450,036 - RR42_RS20530 0.79 -0.2 4,450,036 - RR42_RS20530 0.79 +0.1 4,450,036 - RR42_RS20530 0.79 -0.9 4,450,036 - RR42_RS20530 0.79 -0.4 4,450,036 - RR42_RS20530 0.79 -0.2 4,450,278 + -0.3 4,450,278 + -0.0 4,450,278 + -0.1 4,450,278 + +0.6 4,450,279 - +0.0 4,450,279 - -1.2 4,450,305 + -0.4 4,450,305 + -0.0 4,450,305 + +0.1 4,450,305 + -0.7 4,450,306 - +0.3 4,450,306 - +1.1 4,450,306 - -0.8 4,450,306 - -0.7 4,450,306 - -0.0 4,450,306 - -0.0 4,450,306 - -0.4 4,450,306 - +0.5 4,450,309 + -0.0 4,450,309 + -0.5 4,450,309 + -0.5 4,450,309 + -0.3 4,450,309 + -0.2 4,450,309 + -0.0 4,450,310 - -0.0 4,450,310 - +0.1 4,450,310 - +0.2 4,450,310 - +1.0 4,450,310 - -0.8 4,450,383 - -0.6 4,450,423 + -0.3 4,450,423 + -0.4 4,450,423 + +0.6 4,450,423 + +0.1 4,450,424 - -0.5 4,450,424 - +0.5 4,450,424 - +1.1 4,450,424 - +1.2 4,450,429 + -0.1 4,450,429 + -1.0 4,450,430 - +0.4 4,450,430 - -0.7 4,450,430 - +0.1 4,450,442 - -0.5 4,450,587 + -0.6 4,450,587 + -1.3 4,450,756 + +0.9 4,450,757 - -0.9 4,450,824 - -0.2 4,450,824 - +0.6 4,450,919 + +1.0 4,450,919 + +0.3 4,450,920 - +1.1 4,450,920 - -0.3 4,450,920 - +0.4 4,450,925 + -1.4 4,450,926 - +1.2 4,450,926 - -0.2 4,450,926 - -0.6 4,450,927 + +0.8 4,450,981 + +0.4 4,450,981 + +0.6 4,451,006 - +0.3 4,451,016 + +0.0 4,451,017 - +0.4 4,451,148 - RR42_RS20535 0.15 -0.3 4,451,255 + RR42_RS20535 0.26 +0.8 4,451,256 - RR42_RS20535 0.26 +0.2 4,451,256 - RR42_RS20535 0.26 +0.8 4,451,358 + RR42_RS20535 0.36 +2.4 4,451,358 + RR42_RS20535 0.36 -0.2 4,451,359 - RR42_RS20535 0.36 +0.1 4,451,359 - RR42_RS20535 0.36 +1.4
Or see this region's nucleotide sequence