Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20230 and RR42_RS20235 are separated by 266 nucleotides RR42_RS20235 and RR42_RS20240 are separated by 10 nucleotides
RR42_RS20230: RR42_RS20230 - LysR family transcriptional regulator, at 4,375,341 to 4,376,285
_RS20230
RR42_RS20235: RR42_RS20235 - metal-dependent hydrolase, at 4,376,552 to 4,377,295
_RS20235
RR42_RS20240: RR42_RS20240 - hypothetical protein, at 4,377,306 to 4,379,093
_RS20240
Position (kb) 4376
4377
4378 Strain fitness (log2 ratio) -1
0
1
2 at 4375.660 kb on + strand, within RR42_RS20230 at 4375.661 kb on - strand, within RR42_RS20230 at 4375.661 kb on - strand, within RR42_RS20230 at 4375.661 kb on - strand, within RR42_RS20230 at 4375.818 kb on + strand, within RR42_RS20230 at 4375.819 kb on - strand, within RR42_RS20230 at 4375.929 kb on + strand, within RR42_RS20230 at 4375.930 kb on - strand, within RR42_RS20230 at 4375.930 kb on - strand, within RR42_RS20230 at 4375.966 kb on + strand, within RR42_RS20230 at 4375.966 kb on + strand, within RR42_RS20230 at 4375.968 kb on + strand, within RR42_RS20230 at 4375.969 kb on - strand, within RR42_RS20230 at 4375.969 kb on - strand, within RR42_RS20230 at 4375.969 kb on - strand, within RR42_RS20230 at 4376.055 kb on - strand, within RR42_RS20230 at 4376.289 kb on + strand at 4376.289 kb on + strand at 4376.296 kb on + strand at 4376.296 kb on + strand at 4376.554 kb on - strand at 4376.580 kb on + strand at 4376.580 kb on + strand at 4376.658 kb on + strand, within RR42_RS20235 at 4376.658 kb on + strand, within RR42_RS20235 at 4376.659 kb on - strand, within RR42_RS20235 at 4376.872 kb on + strand, within RR42_RS20235 at 4376.873 kb on - strand, within RR42_RS20235 at 4376.919 kb on + strand, within RR42_RS20235 at 4376.919 kb on + strand, within RR42_RS20235 at 4376.920 kb on - strand, within RR42_RS20235 at 4377.099 kb on + strand, within RR42_RS20235 at 4377.255 kb on + strand at 4377.287 kb on - strand at 4377.308 kb on - strand at 4377.325 kb on + strand at 4377.325 kb on + strand at 4377.386 kb on + strand at 4377.518 kb on - strand, within RR42_RS20240 at 4377.543 kb on + strand, within RR42_RS20240 at 4377.543 kb on + strand, within RR42_RS20240 at 4377.544 kb on - strand, within RR42_RS20240 at 4377.544 kb on - strand, within RR42_RS20240 at 4377.631 kb on + strand, within RR42_RS20240 at 4377.631 kb on + strand, within RR42_RS20240 at 4377.631 kb on + strand, within RR42_RS20240 at 4377.631 kb on + strand, within RR42_RS20240 at 4377.631 kb on + strand, within RR42_RS20240 at 4377.632 kb on - strand, within RR42_RS20240 at 4377.632 kb on - strand, within RR42_RS20240 at 4377.632 kb on - strand, within RR42_RS20240 at 4377.632 kb on - strand, within RR42_RS20240 at 4377.632 kb on - strand, within RR42_RS20240 at 4377.632 kb on - strand, within RR42_RS20240 at 4377.632 kb on - strand, within RR42_RS20240 at 4377.889 kb on + strand, within RR42_RS20240 at 4377.889 kb on + strand, within RR42_RS20240 at 4377.890 kb on - strand, within RR42_RS20240 at 4378.187 kb on - strand, within RR42_RS20240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 4,375,660 + RR42_RS20230 0.34 +0.4 4,375,661 - RR42_RS20230 0.34 +0.3 4,375,661 - RR42_RS20230 0.34 +0.9 4,375,661 - RR42_RS20230 0.34 -0.4 4,375,818 + RR42_RS20230 0.50 +0.6 4,375,819 - RR42_RS20230 0.51 +0.6 4,375,929 + RR42_RS20230 0.62 -0.2 4,375,930 - RR42_RS20230 0.62 +0.9 4,375,930 - RR42_RS20230 0.62 +0.8 4,375,966 + RR42_RS20230 0.66 -0.1 4,375,966 + RR42_RS20230 0.66 +1.1 4,375,968 + RR42_RS20230 0.66 +0.6 4,375,969 - RR42_RS20230 0.66 +0.8 4,375,969 - RR42_RS20230 0.66 +0.2 4,375,969 - RR42_RS20230 0.66 +0.3 4,376,055 - RR42_RS20230 0.76 +0.4 4,376,289 + +0.3 4,376,289 + -0.1 4,376,296 + -0.1 4,376,296 + +0.4 4,376,554 - +0.3 4,376,580 + +0.3 4,376,580 + -0.9 4,376,658 + RR42_RS20235 0.14 +0.8 4,376,658 + RR42_RS20235 0.14 +0.2 4,376,659 - RR42_RS20235 0.14 -1.7 4,376,872 + RR42_RS20235 0.43 -0.2 4,376,873 - RR42_RS20235 0.43 -0.5 4,376,919 + RR42_RS20235 0.49 -0.8 4,376,919 + RR42_RS20235 0.49 +0.2 4,376,920 - RR42_RS20235 0.49 -1.2 4,377,099 + RR42_RS20235 0.74 +0.3 4,377,255 + +1.1 4,377,287 - +0.9 4,377,308 - -1.2 4,377,325 + -0.1 4,377,325 + -0.0 4,377,386 + -0.4 4,377,518 - RR42_RS20240 0.12 -0.6 4,377,543 + RR42_RS20240 0.13 -0.1 4,377,543 + RR42_RS20240 0.13 +0.0 4,377,544 - RR42_RS20240 0.13 -0.2 4,377,544 - RR42_RS20240 0.13 -0.1 4,377,631 + RR42_RS20240 0.18 -1.1 4,377,631 + RR42_RS20240 0.18 +0.8 4,377,631 + RR42_RS20240 0.18 -0.4 4,377,631 + RR42_RS20240 0.18 -0.7 4,377,631 + RR42_RS20240 0.18 +0.2 4,377,632 - RR42_RS20240 0.18 +0.9 4,377,632 - RR42_RS20240 0.18 +0.3 4,377,632 - RR42_RS20240 0.18 -0.4 4,377,632 - RR42_RS20240 0.18 +1.9 4,377,632 - RR42_RS20240 0.18 +0.2 4,377,632 - RR42_RS20240 0.18 -0.2 4,377,632 - RR42_RS20240 0.18 +0.2 4,377,889 + RR42_RS20240 0.33 -0.5 4,377,889 + RR42_RS20240 0.33 +0.6 4,377,890 - RR42_RS20240 0.33 +0.6 4,378,187 - RR42_RS20240 0.49 +0.3
Or see this region's nucleotide sequence