Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS18500

Experiment: R2A with Sisomicin 0.035 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Sisomicin 0.035 mg/ml
4,002,784	+				-0.7
4,002,784	+				+0.3
4,002,785	-				+0.3
4,002,785	-				+0.3
4,002,804	+				-0.2
4,002,858	+		RR42_RS18495	0.14	+0.2
4,002,858	+		RR42_RS18495	0.14	+0.3
4,002,859	-		RR42_RS18495	0.14	-0.0
4,002,859	-		RR42_RS18495	0.14	-0.4
4,002,861	+		RR42_RS18495	0.15	+0.2
4,002,874	-		RR42_RS18495	0.21	-0.1
4,002,879	+		RR42_RS18495	0.23	-2.7
4,002,879	+		RR42_RS18495	0.23	+0.4
4,002,879	+		RR42_RS18495	0.23	+0.3
4,002,879	+		RR42_RS18495	0.23	+0.1
4,002,879	+		RR42_RS18495	0.23	-0.2
4,002,879	+		RR42_RS18495	0.23	-0.1
4,002,880	-		RR42_RS18495	0.23	-0.4
4,002,880	-		RR42_RS18495	0.23	-0.7
4,002,880	-		RR42_RS18495	0.23	-0.1
4,002,880	-		RR42_RS18495	0.23	+0.1
4,002,882	+		RR42_RS18495	0.24	+1.0
4,002,882	+		RR42_RS18495	0.24	-0.4
4,002,882	+		RR42_RS18495	0.24	+1.3
4,002,882	+		RR42_RS18495	0.24	+0.6
4,002,882	+		RR42_RS18495	0.24	+0.5
4,002,883	-		RR42_RS18495	0.25	+1.4
4,002,883	-		RR42_RS18495	0.25	+0.1
4,002,891	+		RR42_RS18495	0.28	-0.6
4,002,892	-		RR42_RS18495	0.29	+1.7
4,002,927	+		RR42_RS18495	0.44	-0.5
4,002,966	+		RR42_RS18495	0.61	+0.0
4,002,972	+		RR42_RS18495	0.64	+0.8
4,003,077	+				-0.0
4,003,095	-				-1.0
4,003,103	+				-0.3
4,003,104	-				-0.0
4,003,104	-				+0.7
4,003,105	+				+0.3
4,003,105	+				-0.4
4,003,106	-				+1.0
4,003,106	-				-0.1
4,003,121	-				-0.1
4,003,121	-				-0.1
4,003,124	-				-1.1
4,003,141	+				+0.4
4,003,141	+				-0.3
4,003,141	+				+0.7
4,003,152	-				+0.9
4,003,177	+				+1.6
4,003,178	-				-0.8
4,003,178	-				-0.2
4,003,252	+				+0.4
4,003,253	-				+0.3
4,003,263	-				-1.7
4,003,280	+				+0.0
4,003,280	+				-0.1
4,003,281	-				+0.1
4,003,281	-				+0.8
4,003,282	+				+0.9
4,003,282	+				-0.9
4,003,282	+				-1.0
4,003,283	-				+0.5
4,003,283	-				+0.4
4,003,516	+		RR42_RS18500	0.21	-0.8
4,003,517	-		RR42_RS18500	0.21	+0.5
4,003,528	+		RR42_RS18500	0.22	+0.5
4,003,528	+		RR42_RS18500	0.22	-0.2
4,003,603	+		RR42_RS18500	0.27	-0.2
4,003,603	+		RR42_RS18500	0.27	-0.8
4,003,797	-		RR42_RS18500	0.40	-1.0
4,003,797	-		RR42_RS18500	0.40	-0.6
4,004,284	+		RR42_RS18500	0.73	-1.1
4,004,285	-		RR42_RS18500	0.73	-1.3
4,004,336	+		RR42_RS18500	0.76	-1.5
4,004,337	-		RR42_RS18500	0.76	-0.1
4,004,407	-		RR42_RS18500	0.81	+0.4
4,004,565	-				-1.0
4,004,565	-				-2.6
4,004,565	-				+1.4
4,004,583	+				+0.7
4,004,757	+				+0.5
4,004,757	+				+0.3
4,004,758	-				-0.7
4,004,758	-				+0.2
4,005,153	+		RR42_RS18505	0.36	+0.6
4,005,153	+		RR42_RS18505	0.36	+0.3
4,005,215	-		RR42_RS18505	0.41	+0.8
4,005,217	+		RR42_RS18505	0.41	+1.0
4,005,218	-		RR42_RS18505	0.41	+0.1
4,005,218	-		RR42_RS18505	0.41	-0.0
4,005,218	-		RR42_RS18505	0.41	-1.1
4,005,229	-		RR42_RS18505	0.42	+0.9
4,005,321	+		RR42_RS18505	0.50	+0.1
4,005,322	-		RR42_RS18505	0.50	+0.1
4,005,322	-		RR42_RS18505	0.50	+0.9
4,005,322	-		RR42_RS18505	0.50	-0.1
4,005,360	+		RR42_RS18505	0.53	+0.2
4,005,361	-		RR42_RS18505	0.53	+1.4
4,005,361	-		RR42_RS18505	0.53	-0.6
4,005,361	-		RR42_RS18505	0.53	+0.3
4,005,475	+		RR42_RS18505	0.62	+1.7
4,005,580	-		RR42_RS18505	0.71	-0.3
4,005,580	-		RR42_RS18505	0.71	-0.4
4,005,580	-		RR42_RS18505	0.71	+2.9