Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17395 and RR42_RS17400 are separated by 40 nucleotides RR42_RS17400 and RR42_RS17405 are separated by 173 nucleotides RR42_RS17405 and RR42_RS17410 are separated by 240 nucleotides
RR42_RS17395: RR42_RS17395 - S-adenosylmethionine tRNA ribosyltransferase, at 3,765,088 to 3,766,140
_RS17395
RR42_RS17400: RR42_RS17400 - queuine tRNA-ribosyltransferase, at 3,766,181 to 3,767,332
_RS17400
RR42_RS17405: RR42_RS17405 - preprotein translocase subunit YajC, at 3,767,506 to 3,767,835
_RS17405
RR42_RS17410: RR42_RS17410 - preprotein translocase subunit SecD, at 3,768,076 to 3,769,947
_RS17410
Position (kb)
3766
3767
3768 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3765.247 kb on + strand, within RR42_RS17395 at 3765.248 kb on - strand, within RR42_RS17395 at 3765.248 kb on - strand, within RR42_RS17395 at 3765.386 kb on + strand, within RR42_RS17395 at 3765.386 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.778 kb on + strand, within RR42_RS17395 at 3766.093 kb on + strand at 3766.093 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.287 kb on - strand at 3766.287 kb on - strand at 3766.826 kb on + strand, within RR42_RS17400 at 3767.111 kb on + strand, within RR42_RS17400 at 3767.111 kb on + strand, within RR42_RS17400 at 3767.112 kb on - strand, within RR42_RS17400 at 3767.112 kb on - strand, within RR42_RS17400 at 3767.112 kb on - strand, within RR42_RS17400 at 3767.113 kb on + strand, within RR42_RS17400 at 3767.113 kb on + strand, within RR42_RS17400 at 3767.114 kb on - strand, within RR42_RS17400 at 3767.114 kb on - strand, within RR42_RS17400 at 3767.138 kb on + strand, within RR42_RS17400 at 3767.208 kb on - strand, within RR42_RS17400 at 3767.210 kb on + strand, within RR42_RS17400 at 3767.409 kb on + strand at 3767.409 kb on + strand at 3767.409 kb on + strand at 3767.410 kb on - strand at 3767.412 kb on + strand at 3767.412 kb on + strand at 3767.412 kb on + strand at 3767.412 kb on + strand at 3767.413 kb on - strand at 3767.419 kb on + strand at 3767.419 kb on + strand at 3767.419 kb on + strand at 3767.420 kb on - strand at 3767.462 kb on + strand at 3767.462 kb on + strand at 3767.475 kb on + strand at 3767.475 kb on + strand at 3767.517 kb on + strand at 3767.583 kb on + strand, within RR42_RS17405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,765,247 + RR42_RS17395 0.15 -1.4 3,765,248 - RR42_RS17395 0.15 -1.9 3,765,248 - RR42_RS17395 0.15 -0.5 3,765,386 + RR42_RS17395 0.28 -1.7 3,765,386 + RR42_RS17395 0.28 -1.9 3,765,544 + RR42_RS17395 0.43 -0.1 3,765,544 + RR42_RS17395 0.43 -0.3 3,765,544 + RR42_RS17395 0.43 +0.3 3,765,544 + RR42_RS17395 0.43 -3.1 3,765,544 + RR42_RS17395 0.43 -0.4 3,765,544 + RR42_RS17395 0.43 -1.1 3,765,545 - RR42_RS17395 0.43 -0.7 3,765,545 - RR42_RS17395 0.43 -1.1 3,765,545 - RR42_RS17395 0.43 -2.6 3,765,545 - RR42_RS17395 0.43 -1.1 3,765,778 + RR42_RS17395 0.66 -0.8 3,766,093 + -0.7 3,766,093 + -0.1 3,766,286 + -1.2 3,766,286 + -3.5 3,766,286 + -3.0 3,766,286 + -2.7 3,766,286 + -1.7 3,766,287 - -2.0 3,766,287 - +1.0 3,766,826 + RR42_RS17400 0.56 -0.1 3,767,111 + RR42_RS17400 0.81 -1.2 3,767,111 + RR42_RS17400 0.81 -0.7 3,767,112 - RR42_RS17400 0.81 -0.6 3,767,112 - RR42_RS17400 0.81 -0.7 3,767,112 - RR42_RS17400 0.81 +1.3 3,767,113 + RR42_RS17400 0.81 -1.5 3,767,113 + RR42_RS17400 0.81 -0.5 3,767,114 - RR42_RS17400 0.81 -1.1 3,767,114 - RR42_RS17400 0.81 -1.3 3,767,138 + RR42_RS17400 0.83 -1.7 3,767,208 - RR42_RS17400 0.89 -0.6 3,767,210 + RR42_RS17400 0.89 -0.5 3,767,409 + +0.9 3,767,409 + +3.0 3,767,409 + -2.0 3,767,410 - -0.2 3,767,412 + -0.3 3,767,412 + +1.8 3,767,412 + -1.1 3,767,412 + -0.9 3,767,413 - +0.0 3,767,419 + -0.3 3,767,419 + -1.8 3,767,419 + -0.2 3,767,420 - -0.7 3,767,462 + -0.9 3,767,462 + -1.4 3,767,475 + +0.5 3,767,475 + -0.6 3,767,517 + -2.4 3,767,583 + RR42_RS17405 0.23 -3.0
Or see this region's nucleotide sequence