Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17365 and RR42_RS17370 are separated by 92 nucleotides RR42_RS17370 and RR42_RS17375 are separated by 77 nucleotides RR42_RS17375 and RR42_RS17380 are separated by 77 nucleotides RR42_RS17380 and RR42_RS17385 are separated by 411 nucleotides
RR42_RS17365: RR42_RS17365 - 4-hydroxybenzoate polyprenyltransferase, at 3,757,948 to 3,758,841
_RS17365
RR42_RS17370: RR42_RS17370 - ferritin, at 3,758,934 to 3,759,419
_RS17370
RR42_RS17375: RR42_RS17375 - hypothetical protein, at 3,759,497 to 3,759,691
_RS17375
RR42_RS17380: RR42_RS17380 - catalase, at 3,759,769 to 3,761,214
_RS17380
RR42_RS17385: RR42_RS17385 - LysR family transcriptional regulator, at 3,761,626 to 3,762,579
_RS17385
Position (kb)
3759
3760
3761
3762 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3759.422 kb on + strand at 3759.563 kb on + strand, within RR42_RS17375 at 3759.770 kb on + strand at 3759.771 kb on - strand at 3759.771 kb on - strand at 3759.893 kb on + strand at 3759.894 kb on - strand at 3759.950 kb on + strand, within RR42_RS17380 at 3759.951 kb on - strand, within RR42_RS17380 at 3759.951 kb on - strand, within RR42_RS17380 at 3760.061 kb on + strand, within RR42_RS17380 at 3760.061 kb on + strand, within RR42_RS17380 at 3760.062 kb on - strand, within RR42_RS17380 at 3760.119 kb on + strand, within RR42_RS17380 at 3760.119 kb on + strand, within RR42_RS17380 at 3760.119 kb on + strand, within RR42_RS17380 at 3760.119 kb on + strand, within RR42_RS17380 at 3760.120 kb on - strand, within RR42_RS17380 at 3760.120 kb on - strand, within RR42_RS17380 at 3760.120 kb on - strand, within RR42_RS17380 at 3760.199 kb on + strand, within RR42_RS17380 at 3760.200 kb on - strand, within RR42_RS17380 at 3760.298 kb on + strand, within RR42_RS17380 at 3760.298 kb on + strand, within RR42_RS17380 at 3760.299 kb on - strand, within RR42_RS17380 at 3760.349 kb on + strand, within RR42_RS17380 at 3760.398 kb on - strand, within RR42_RS17380 at 3760.398 kb on - strand, within RR42_RS17380 at 3760.398 kb on - strand, within RR42_RS17380 at 3760.617 kb on + strand, within RR42_RS17380 at 3760.618 kb on - strand, within RR42_RS17380 at 3760.618 kb on - strand, within RR42_RS17380 at 3760.618 kb on - strand, within RR42_RS17380 at 3760.618 kb on - strand, within RR42_RS17380 at 3760.646 kb on + strand, within RR42_RS17380 at 3760.646 kb on + strand, within RR42_RS17380 at 3760.678 kb on + strand, within RR42_RS17380 at 3760.678 kb on + strand, within RR42_RS17380 at 3760.678 kb on + strand, within RR42_RS17380 at 3760.678 kb on + strand, within RR42_RS17380 at 3760.863 kb on - strand, within RR42_RS17380 at 3760.863 kb on - strand, within RR42_RS17380 at 3760.963 kb on + strand, within RR42_RS17380 at 3760.964 kb on - strand, within RR42_RS17380 at 3761.293 kb on + strand at 3761.293 kb on + strand at 3761.294 kb on - strand at 3761.294 kb on - strand at 3761.294 kb on - strand at 3761.294 kb on - strand at 3761.306 kb on - strand at 3761.306 kb on - strand at 3761.306 kb on - strand at 3761.499 kb on + strand at 3761.499 kb on + strand at 3761.789 kb on + strand, within RR42_RS17385 at 3761.789 kb on + strand, within RR42_RS17385 at 3761.789 kb on + strand, within RR42_RS17385 at 3761.790 kb on - strand, within RR42_RS17385 at 3761.790 kb on - strand, within RR42_RS17385 at 3761.874 kb on + strand, within RR42_RS17385 at 3761.874 kb on + strand, within RR42_RS17385 at 3761.874 kb on + strand, within RR42_RS17385 at 3761.874 kb on + strand, within RR42_RS17385 at 3761.875 kb on - strand, within RR42_RS17385 at 3761.948 kb on - strand, within RR42_RS17385 at 3762.098 kb on + strand, within RR42_RS17385 at 3762.099 kb on - strand, within RR42_RS17385 at 3762.197 kb on + strand, within RR42_RS17385 at 3762.197 kb on + strand, within RR42_RS17385 at 3762.197 kb on + strand, within RR42_RS17385 at 3762.197 kb on + strand, within RR42_RS17385 at 3762.198 kb on - strand, within RR42_RS17385 at 3762.198 kb on - strand, within RR42_RS17385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,759,422 + -0.8 3,759,563 + RR42_RS17375 0.34 -0.3 3,759,770 + -0.6 3,759,771 - +0.3 3,759,771 - +1.0 3,759,893 + +0.6 3,759,894 - -0.3 3,759,950 + RR42_RS17380 0.13 -0.5 3,759,951 - RR42_RS17380 0.13 -1.1 3,759,951 - RR42_RS17380 0.13 -0.4 3,760,061 + RR42_RS17380 0.20 -0.4 3,760,061 + RR42_RS17380 0.20 -0.2 3,760,062 - RR42_RS17380 0.20 -0.4 3,760,119 + RR42_RS17380 0.24 +1.0 3,760,119 + RR42_RS17380 0.24 -0.7 3,760,119 + RR42_RS17380 0.24 -0.1 3,760,119 + RR42_RS17380 0.24 +0.6 3,760,120 - RR42_RS17380 0.24 -0.9 3,760,120 - RR42_RS17380 0.24 -0.3 3,760,120 - RR42_RS17380 0.24 -0.3 3,760,199 + RR42_RS17380 0.30 -1.3 3,760,200 - RR42_RS17380 0.30 +0.1 3,760,298 + RR42_RS17380 0.37 -0.6 3,760,298 + RR42_RS17380 0.37 -0.6 3,760,299 - RR42_RS17380 0.37 -0.1 3,760,349 + RR42_RS17380 0.40 +0.1 3,760,398 - RR42_RS17380 0.43 -0.8 3,760,398 - RR42_RS17380 0.43 -1.2 3,760,398 - RR42_RS17380 0.43 -0.2 3,760,617 + RR42_RS17380 0.59 +0.2 3,760,618 - RR42_RS17380 0.59 -0.4 3,760,618 - RR42_RS17380 0.59 -1.0 3,760,618 - RR42_RS17380 0.59 -1.9 3,760,618 - RR42_RS17380 0.59 +0.3 3,760,646 + RR42_RS17380 0.61 +0.5 3,760,646 + RR42_RS17380 0.61 +0.6 3,760,678 + RR42_RS17380 0.63 +0.2 3,760,678 + RR42_RS17380 0.63 -0.9 3,760,678 + RR42_RS17380 0.63 +0.1 3,760,678 + RR42_RS17380 0.63 +0.8 3,760,863 - RR42_RS17380 0.76 -0.6 3,760,863 - RR42_RS17380 0.76 +0.1 3,760,963 + RR42_RS17380 0.83 +0.1 3,760,964 - RR42_RS17380 0.83 +0.4 3,761,293 + -2.3 3,761,293 + +0.8 3,761,294 - +2.1 3,761,294 - -0.1 3,761,294 - -0.6 3,761,294 - +1.3 3,761,306 - +0.6 3,761,306 - -0.0 3,761,306 - -0.3 3,761,499 + -1.3 3,761,499 + -0.6 3,761,789 + RR42_RS17385 0.17 +0.1 3,761,789 + RR42_RS17385 0.17 -0.0 3,761,789 + RR42_RS17385 0.17 -0.0 3,761,790 - RR42_RS17385 0.17 +0.6 3,761,790 - RR42_RS17385 0.17 +2.3 3,761,874 + RR42_RS17385 0.26 +0.8 3,761,874 + RR42_RS17385 0.26 -0.2 3,761,874 + RR42_RS17385 0.26 +0.5 3,761,874 + RR42_RS17385 0.26 -0.9 3,761,875 - RR42_RS17385 0.26 +0.8 3,761,948 - RR42_RS17385 0.34 -1.5 3,762,098 + RR42_RS17385 0.49 -0.5 3,762,099 - RR42_RS17385 0.50 +1.7 3,762,197 + RR42_RS17385 0.60 -1.3 3,762,197 + RR42_RS17385 0.60 +0.0 3,762,197 + RR42_RS17385 0.60 +0.1 3,762,197 + RR42_RS17385 0.60 +0.4 3,762,198 - RR42_RS17385 0.60 -0.0 3,762,198 - RR42_RS17385 0.60 +0.4
Or see this region's nucleotide sequence