Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17285 and RR42_RS17290 are separated by 13 nucleotides RR42_RS17290 and RR42_RS17295 are separated by 44 nucleotides RR42_RS17295 and RR42_RS17300 are separated by 253 nucleotides
RR42_RS17285: RR42_RS17285 - 2-hydroxy-acid oxidase, at 3,742,813 to 3,744,231
_RS17285
RR42_RS17290: RR42_RS17290 - LysR family transcriptional regulator, at 3,744,245 to 3,745,135
_RS17290
RR42_RS17295: RR42_RS17295 - acetyltransferase, at 3,745,180 to 3,745,707
_RS17295
RR42_RS17300: RR42_RS17300 - D-lactate dehydrogenase, FAD-linked subunit GlcD (EC 1.1.99.6) (from data) , at 3,745,961 to 3,747,454
_RS17300
Position (kb)
3744
3745
3746 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3744.082 kb on - strand, within RR42_RS17285 at 3744.458 kb on + strand, within RR42_RS17290 at 3744.459 kb on - strand, within RR42_RS17290 at 3744.515 kb on + strand, within RR42_RS17290 at 3744.516 kb on - strand, within RR42_RS17290 at 3744.516 kb on - strand, within RR42_RS17290 at 3744.770 kb on + strand, within RR42_RS17290 at 3744.771 kb on - strand, within RR42_RS17290 at 3744.771 kb on - strand, within RR42_RS17290 at 3744.771 kb on - strand, within RR42_RS17290 at 3744.771 kb on - strand, within RR42_RS17290 at 3744.879 kb on + strand, within RR42_RS17290 at 3745.053 kb on - strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.055 kb on + strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.056 kb on - strand at 3745.100 kb on + strand at 3745.134 kb on - strand at 3745.134 kb on - strand at 3745.403 kb on + strand, within RR42_RS17295 at 3745.443 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.456 kb on + strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.457 kb on - strand, within RR42_RS17295 at 3745.594 kb on + strand, within RR42_RS17295 at 3745.595 kb on - strand, within RR42_RS17295 at 3745.642 kb on + strand, within RR42_RS17295 at 3745.642 kb on + strand, within RR42_RS17295 at 3745.643 kb on - strand, within RR42_RS17295 at 3745.643 kb on - strand, within RR42_RS17295 at 3745.643 kb on - strand, within RR42_RS17295 at 3745.658 kb on - strand at 3745.705 kb on + strand at 3745.705 kb on + strand at 3745.705 kb on + strand at 3745.706 kb on - strand at 3745.742 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.894 kb on + strand at 3745.895 kb on - strand at 3745.948 kb on - strand at 3746.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,744,082 - RR42_RS17285 0.89 -2.1 3,744,458 + RR42_RS17290 0.24 +0.4 3,744,459 - RR42_RS17290 0.24 +0.0 3,744,515 + RR42_RS17290 0.30 +1.0 3,744,516 - RR42_RS17290 0.30 +0.7 3,744,516 - RR42_RS17290 0.30 -0.1 3,744,770 + RR42_RS17290 0.59 -0.3 3,744,771 - RR42_RS17290 0.59 -0.8 3,744,771 - RR42_RS17290 0.59 +0.8 3,744,771 - RR42_RS17290 0.59 +1.1 3,744,771 - RR42_RS17290 0.59 +0.8 3,744,879 + RR42_RS17290 0.71 +0.6 3,745,053 - -0.6 3,745,055 + +2.1 3,745,055 + -0.8 3,745,055 + -2.5 3,745,055 + -2.1 3,745,055 + +0.3 3,745,056 - +1.2 3,745,056 - +0.4 3,745,056 - -1.2 3,745,056 - +0.0 3,745,056 - +1.1 3,745,056 - +0.6 3,745,056 - -0.8 3,745,056 - +0.5 3,745,056 - -0.8 3,745,100 + +0.2 3,745,134 - +0.5 3,745,134 - +0.5 3,745,403 + RR42_RS17295 0.42 +0.2 3,745,443 + RR42_RS17295 0.50 -1.2 3,745,456 + RR42_RS17295 0.52 +0.2 3,745,456 + RR42_RS17295 0.52 +0.1 3,745,456 + RR42_RS17295 0.52 +0.9 3,745,456 + RR42_RS17295 0.52 +0.8 3,745,456 + RR42_RS17295 0.52 +0.2 3,745,456 + RR42_RS17295 0.52 +0.2 3,745,456 + RR42_RS17295 0.52 -0.2 3,745,456 + RR42_RS17295 0.52 +0.2 3,745,457 - RR42_RS17295 0.52 +0.4 3,745,457 - RR42_RS17295 0.52 -0.8 3,745,457 - RR42_RS17295 0.52 -0.0 3,745,457 - RR42_RS17295 0.52 +0.3 3,745,457 - RR42_RS17295 0.52 +0.7 3,745,457 - RR42_RS17295 0.52 -1.0 3,745,457 - RR42_RS17295 0.52 -1.3 3,745,594 + RR42_RS17295 0.78 -0.1 3,745,595 - RR42_RS17295 0.79 +1.0 3,745,642 + RR42_RS17295 0.88 +0.5 3,745,642 + RR42_RS17295 0.88 +0.2 3,745,643 - RR42_RS17295 0.88 +0.1 3,745,643 - RR42_RS17295 0.88 +0.3 3,745,643 - RR42_RS17295 0.88 -0.8 3,745,658 - +0.1 3,745,705 + -0.6 3,745,705 + +0.0 3,745,705 + +0.7 3,745,706 - +0.8 3,745,742 + -1.7 3,745,894 + -0.7 3,745,894 + +0.2 3,745,894 + +0.4 3,745,894 + +0.6 3,745,894 + +0.8 3,745,895 - +0.4 3,745,948 - -0.3 3,746,085 - +1.0
Or see this region's nucleotide sequence