Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16735 and RR42_RS16740 are separated by 219 nucleotides RR42_RS16740 and RR42_RS16745 are separated by 79 nucleotides RR42_RS16745 and RR42_RS16750 are separated by 56 nucleotides RR42_RS16750 and RR42_RS16755 are separated by 5 nucleotides
RR42_RS16735: RR42_RS16735 - LytR family transcriptional regulator, at 3,621,380 to 3,622,198
_RS16735
RR42_RS16740: RR42_RS16740 - hypothetical protein, at 3,622,418 to 3,622,684
_RS16740
RR42_RS16745: RR42_RS16745 - copper resistance protein CopQ, at 3,622,764 to 3,623,000
_RS16745
RR42_RS16750: RR42_RS16750 - transcriptional regulator, at 3,623,057 to 3,623,641
_RS16750
RR42_RS16755: RR42_RS16755 - FAD-linked oxidase, at 3,623,647 to 3,625,200
_RS16755
Position (kb)
3622
3623 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3621.484 kb on - strand, within RR42_RS16735 at 3621.659 kb on + strand, within RR42_RS16735 at 3621.660 kb on - strand, within RR42_RS16735 at 3621.660 kb on - strand, within RR42_RS16735 at 3621.695 kb on + strand, within RR42_RS16735 at 3621.702 kb on + strand, within RR42_RS16735 at 3621.702 kb on + strand, within RR42_RS16735 at 3621.962 kb on + strand, within RR42_RS16735 at 3621.962 kb on + strand, within RR42_RS16735 at 3621.963 kb on - strand, within RR42_RS16735 at 3621.963 kb on - strand, within RR42_RS16735 at 3622.074 kb on + strand, within RR42_RS16735 at 3622.172 kb on + strand at 3622.197 kb on - strand at 3622.307 kb on - strand at 3622.428 kb on + strand at 3622.506 kb on - strand, within RR42_RS16740 at 3622.542 kb on - strand, within RR42_RS16740 at 3622.628 kb on + strand, within RR42_RS16740 at 3622.629 kb on - strand, within RR42_RS16740 at 3622.698 kb on - strand at 3622.980 kb on + strand at 3623.409 kb on + strand, within RR42_RS16750 at 3623.469 kb on + strand, within RR42_RS16750 at 3623.469 kb on + strand, within RR42_RS16750 at 3623.470 kb on - strand, within RR42_RS16750 at 3623.567 kb on - strand, within RR42_RS16750 at 3623.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,621,484 - RR42_RS16735 0.13 +1.8 3,621,659 + RR42_RS16735 0.34 +0.8 3,621,660 - RR42_RS16735 0.34 -0.5 3,621,660 - RR42_RS16735 0.34 -0.7 3,621,695 + RR42_RS16735 0.38 +0.3 3,621,702 + RR42_RS16735 0.39 +0.4 3,621,702 + RR42_RS16735 0.39 -0.6 3,621,962 + RR42_RS16735 0.71 +0.2 3,621,962 + RR42_RS16735 0.71 -1.6 3,621,963 - RR42_RS16735 0.71 +3.8 3,621,963 - RR42_RS16735 0.71 +0.5 3,622,074 + RR42_RS16735 0.85 +2.4 3,622,172 + +2.2 3,622,197 - +0.1 3,622,307 - +0.2 3,622,428 + -0.3 3,622,506 - RR42_RS16740 0.33 -0.5 3,622,542 - RR42_RS16740 0.46 -1.2 3,622,628 + RR42_RS16740 0.79 +2.2 3,622,629 - RR42_RS16740 0.79 -0.5 3,622,698 - +1.2 3,622,980 + +1.2 3,623,409 + RR42_RS16750 0.60 -0.4 3,623,469 + RR42_RS16750 0.70 +0.6 3,623,469 + RR42_RS16750 0.70 -1.1 3,623,470 - RR42_RS16750 0.71 -0.0 3,623,567 - RR42_RS16750 0.87 -1.4 3,623,648 + +2.2
Or see this region's nucleotide sequence