Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16255 and RR42_RS16260 are separated by 11 nucleotides RR42_RS16260 and RR42_RS16265 overlap by 4 nucleotides RR42_RS16265 and RR42_RS16270 are separated by 17 nucleotides
RR42_RS16255: RR42_RS16255 - sulfate adenylyltransferase, at 3,518,915 to 3,519,877
_RS16255
RR42_RS16260: RR42_RS16260 - phosphoadenosine phosphosulfate reductase, at 3,519,889 to 3,520,677
_RS16260
RR42_RS16265: RR42_RS16265 - required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) , at 3,520,674 to 3,521,255
_RS16265
RR42_RS16270: RR42_RS16270 - sulfite reductase, at 3,521,273 to 3,522,952
_RS16270
Position (kb)
3520
3521
3522 Strain fitness (log2 ratio)
-1
0
1
2 at 3519.780 kb on + strand, within RR42_RS16255 at 3519.780 kb on + strand, within RR42_RS16255 at 3519.781 kb on - strand at 3519.781 kb on - strand at 3519.993 kb on + strand, within RR42_RS16260 at 3520.068 kb on - strand, within RR42_RS16260 at 3520.751 kb on - strand, within RR42_RS16265 at 3520.918 kb on + strand, within RR42_RS16265 at 3520.919 kb on - strand, within RR42_RS16265 at 3521.244 kb on - strand at 3521.261 kb on + strand at 3521.388 kb on + strand at 3521.388 kb on + strand at 3521.388 kb on + strand at 3521.389 kb on - strand at 3521.389 kb on - strand at 3521.389 kb on - strand at 3521.522 kb on - strand, within RR42_RS16270 at 3521.588 kb on - strand, within RR42_RS16270 at 3521.588 kb on - strand, within RR42_RS16270 at 3521.619 kb on + strand, within RR42_RS16270 at 3521.620 kb on - strand, within RR42_RS16270 at 3521.781 kb on + strand, within RR42_RS16270 at 3521.781 kb on + strand, within RR42_RS16270 at 3522.078 kb on + strand, within RR42_RS16270 at 3522.213 kb on + strand, within RR42_RS16270 at 3522.214 kb on - strand, within RR42_RS16270 at 3522.243 kb on + strand, within RR42_RS16270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,519,780 + RR42_RS16255 0.90 -0.7 3,519,780 + RR42_RS16255 0.90 -0.7 3,519,781 - +2.2 3,519,781 - +0.3 3,519,993 + RR42_RS16260 0.13 +0.5 3,520,068 - RR42_RS16260 0.23 +0.3 3,520,751 - RR42_RS16265 0.13 +0.9 3,520,918 + RR42_RS16265 0.42 -0.4 3,520,919 - RR42_RS16265 0.42 +0.6 3,521,244 - +0.5 3,521,261 + +0.7 3,521,388 + +1.0 3,521,388 + -0.2 3,521,388 + +2.0 3,521,389 - -0.5 3,521,389 - -0.1 3,521,389 - +0.7 3,521,522 - RR42_RS16270 0.15 -0.4 3,521,588 - RR42_RS16270 0.19 -0.4 3,521,588 - RR42_RS16270 0.19 +0.5 3,521,619 + RR42_RS16270 0.21 -1.0 3,521,620 - RR42_RS16270 0.21 +0.1 3,521,781 + RR42_RS16270 0.30 +0.2 3,521,781 + RR42_RS16270 0.30 +1.1 3,522,078 + RR42_RS16270 0.48 +0.6 3,522,213 + RR42_RS16270 0.56 +1.4 3,522,214 - RR42_RS16270 0.56 +0.3 3,522,243 + RR42_RS16270 0.58 +1.2
Or see this region's nucleotide sequence