Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15770 and RR42_RS15775 are separated by 37 nucleotides RR42_RS15775 and RR42_RS15780 are separated by 2 nucleotides RR42_RS15780 and RR42_RS15785 overlap by 1 nucleotides
RR42_RS15770: RR42_RS15770 - hypothetical protein, at 3,408,389 to 3,409,951
_RS15770
RR42_RS15775: RR42_RS15775 - N-acetylneuraminate synthase, at 3,409,989 to 3,411,038
_RS15775
RR42_RS15780: RR42_RS15780 - hypothetical protein, at 3,411,041 to 3,411,601
_RS15780
RR42_RS15785: RR42_RS15785 - glycosyl transferase family 28, at 3,411,601 to 3,412,686
_RS15785
Position (kb)
3409
3410
3411
3412 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3408.990 kb on + strand, within RR42_RS15770 at 3408.990 kb on + strand, within RR42_RS15770 at 3409.239 kb on + strand, within RR42_RS15770 at 3409.335 kb on + strand, within RR42_RS15770 at 3409.335 kb on + strand, within RR42_RS15770 at 3409.417 kb on + strand, within RR42_RS15770 at 3409.798 kb on + strand at 3409.818 kb on + strand at 3409.818 kb on + strand at 3409.818 kb on + strand at 3409.819 kb on - strand at 3409.848 kb on - strand at 3409.896 kb on + strand at 3409.897 kb on - strand at 3409.927 kb on - strand at 3409.927 kb on - strand at 3409.927 kb on - strand at 3409.954 kb on - strand at 3409.954 kb on - strand at 3409.954 kb on - strand at 3409.957 kb on - strand at 3409.957 kb on - strand at 3409.957 kb on - strand at 3409.959 kb on + strand at 3409.959 kb on + strand at 3409.959 kb on + strand at 3409.959 kb on + strand at 3409.959 kb on + strand at 3409.960 kb on - strand at 3409.960 kb on - strand at 3409.960 kb on - strand at 3409.960 kb on - strand at 3410.207 kb on - strand, within RR42_RS15775 at 3410.207 kb on - strand, within RR42_RS15775 at 3410.387 kb on + strand, within RR42_RS15775 at 3410.387 kb on + strand, within RR42_RS15775 at 3410.388 kb on - strand, within RR42_RS15775 at 3410.475 kb on + strand, within RR42_RS15775 at 3410.478 kb on - strand, within RR42_RS15775 at 3410.621 kb on + strand, within RR42_RS15775 at 3410.708 kb on + strand, within RR42_RS15775 at 3410.709 kb on - strand, within RR42_RS15775 at 3410.785 kb on + strand, within RR42_RS15775 at 3410.881 kb on + strand, within RR42_RS15775 at 3410.882 kb on - strand, within RR42_RS15775 at 3411.042 kb on + strand at 3411.042 kb on + strand at 3411.043 kb on - strand at 3411.045 kb on - strand at 3411.618 kb on - strand at 3411.618 kb on - strand at 3411.772 kb on - strand, within RR42_RS15785 at 3411.772 kb on - strand, within RR42_RS15785 at 3411.774 kb on - strand, within RR42_RS15785 at 3411.949 kb on - strand, within RR42_RS15785 at 3411.949 kb on - strand, within RR42_RS15785 at 3411.949 kb on - strand, within RR42_RS15785 at 3411.976 kb on + strand, within RR42_RS15785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,408,990 + RR42_RS15770 0.38 -1.7 3,408,990 + RR42_RS15770 0.38 -1.0 3,409,239 + RR42_RS15770 0.54 -2.1 3,409,335 + RR42_RS15770 0.61 -1.3 3,409,335 + RR42_RS15770 0.61 -2.0 3,409,417 + RR42_RS15770 0.66 -1.0 3,409,798 + -2.3 3,409,818 + -2.9 3,409,818 + -3.2 3,409,818 + -1.2 3,409,819 - -3.8 3,409,848 - -0.8 3,409,896 + -1.3 3,409,897 - -1.3 3,409,927 - -2.2 3,409,927 - -1.7 3,409,927 - -3.3 3,409,954 - -0.3 3,409,954 - +0.1 3,409,954 - -1.0 3,409,957 - +0.5 3,409,957 - -0.4 3,409,957 - +0.6 3,409,959 + -1.2 3,409,959 + -1.9 3,409,959 + -2.7 3,409,959 + -0.7 3,409,959 + -2.7 3,409,960 - +0.3 3,409,960 - +0.3 3,409,960 - -1.1 3,409,960 - -0.1 3,410,207 - RR42_RS15775 0.21 -2.3 3,410,207 - RR42_RS15775 0.21 -1.4 3,410,387 + RR42_RS15775 0.38 -2.8 3,410,387 + RR42_RS15775 0.38 -1.6 3,410,388 - RR42_RS15775 0.38 -3.4 3,410,475 + RR42_RS15775 0.46 -1.6 3,410,478 - RR42_RS15775 0.47 -1.9 3,410,621 + RR42_RS15775 0.60 -2.3 3,410,708 + RR42_RS15775 0.68 -2.4 3,410,709 - RR42_RS15775 0.69 -2.0 3,410,785 + RR42_RS15775 0.76 -1.2 3,410,881 + RR42_RS15775 0.85 +0.6 3,410,882 - RR42_RS15775 0.85 -2.7 3,411,042 + +0.0 3,411,042 + -0.1 3,411,043 - -1.3 3,411,045 - -0.4 3,411,618 - +1.2 3,411,618 - +0.2 3,411,772 - RR42_RS15785 0.16 -2.4 3,411,772 - RR42_RS15785 0.16 -3.0 3,411,774 - RR42_RS15785 0.16 -1.1 3,411,949 - RR42_RS15785 0.32 -2.2 3,411,949 - RR42_RS15785 0.32 -0.4 3,411,949 - RR42_RS15785 0.32 -3.3 3,411,976 + RR42_RS15785 0.35 -1.0
Or see this region's nucleotide sequence