Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15615 and RR42_RS15620 are separated by 236 nucleotides RR42_RS15620 and RR42_RS15625 are separated by 75 nucleotides RR42_RS15625 and RR42_RS15630 are separated by 107 nucleotides
RR42_RS15615: RR42_RS15615 - aldose epimerase, at 3,373,828 to 3,374,763
_RS15615
RR42_RS15620: RR42_RS15620 - tRNA-Gly, at 3,375,000 to 3,375,074
_RS15620
RR42_RS15625: RR42_RS15625 - tRNA (guanine-N7)-methyltransferase, at 3,375,150 to 3,375,917
_RS15625
RR42_RS15630: RR42_RS15630 - UDP pyrophosphate phosphatase, at 3,376,025 to 3,376,906
_RS15630
Position (kb)
3375
3376 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3374.308 kb on + strand, within RR42_RS15615 at 3374.309 kb on - strand, within RR42_RS15615 at 3374.309 kb on - strand, within RR42_RS15615 at 3374.311 kb on + strand, within RR42_RS15615 at 3374.312 kb on - strand, within RR42_RS15615 at 3374.312 kb on - strand, within RR42_RS15615 at 3374.329 kb on + strand, within RR42_RS15615 at 3374.329 kb on + strand, within RR42_RS15615 at 3374.330 kb on - strand, within RR42_RS15615 at 3374.330 kb on - strand, within RR42_RS15615 at 3374.330 kb on - strand, within RR42_RS15615 at 3374.447 kb on - strand, within RR42_RS15615 at 3374.648 kb on - strand, within RR42_RS15615 at 3374.762 kb on - strand at 3374.762 kb on - strand at 3374.771 kb on + strand at 3374.811 kb on - strand at 3374.924 kb on + strand at 3374.924 kb on + strand at 3374.925 kb on - strand at 3375.010 kb on + strand, within RR42_RS15620 at 3375.010 kb on + strand, within RR42_RS15620 at 3375.012 kb on + strand, within RR42_RS15620 at 3375.012 kb on + strand, within RR42_RS15620 at 3375.012 kb on + strand, within RR42_RS15620 at 3375.022 kb on + strand, within RR42_RS15620 at 3375.040 kb on + strand, within RR42_RS15620 at 3375.041 kb on - strand, within RR42_RS15620 at 3375.339 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.745 kb on - strand, within RR42_RS15625 at 3375.745 kb on - strand, within RR42_RS15625 at 3375.745 kb on - strand, within RR42_RS15625 at 3376.005 kb on - strand at 3376.007 kb on + strand at 3376.007 kb on + strand at 3376.007 kb on + strand at 3376.053 kb on + strand at 3376.053 kb on + strand at 3376.054 kb on - strand at 3376.054 kb on - strand at 3376.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,374,308 + RR42_RS15615 0.51 +1.4 3,374,309 - RR42_RS15615 0.51 -0.5 3,374,309 - RR42_RS15615 0.51 +0.0 3,374,311 + RR42_RS15615 0.52 -0.3 3,374,312 - RR42_RS15615 0.52 +0.6 3,374,312 - RR42_RS15615 0.52 +0.1 3,374,329 + RR42_RS15615 0.54 +0.1 3,374,329 + RR42_RS15615 0.54 +0.8 3,374,330 - RR42_RS15615 0.54 +0.2 3,374,330 - RR42_RS15615 0.54 -0.2 3,374,330 - RR42_RS15615 0.54 +1.4 3,374,447 - RR42_RS15615 0.66 +0.7 3,374,648 - RR42_RS15615 0.88 -0.3 3,374,762 - +0.3 3,374,762 - +0.6 3,374,771 + +3.2 3,374,811 - +0.3 3,374,924 + +0.4 3,374,924 + -0.0 3,374,925 - -0.8 3,375,010 + RR42_RS15620 0.13 +0.8 3,375,010 + RR42_RS15620 0.13 +0.6 3,375,012 + RR42_RS15620 0.16 +0.2 3,375,012 + RR42_RS15620 0.16 +0.3 3,375,012 + RR42_RS15620 0.16 +0.1 3,375,022 + RR42_RS15620 0.29 +0.2 3,375,040 + RR42_RS15620 0.53 +1.9 3,375,041 - RR42_RS15620 0.55 -0.8 3,375,339 + RR42_RS15625 0.25 +1.0 3,375,744 + RR42_RS15625 0.77 +0.7 3,375,744 + RR42_RS15625 0.77 +0.7 3,375,744 + RR42_RS15625 0.77 +1.4 3,375,744 + RR42_RS15625 0.77 +0.8 3,375,745 - RR42_RS15625 0.77 +0.7 3,375,745 - RR42_RS15625 0.77 -0.1 3,375,745 - RR42_RS15625 0.77 +0.2 3,376,005 - -1.3 3,376,007 + -2.1 3,376,007 + +1.4 3,376,007 + -0.5 3,376,053 + -0.4 3,376,053 + +0.5 3,376,054 - -0.5 3,376,054 - +0.8 3,376,054 - -0.8
Or see this region's nucleotide sequence