Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15200

Experiment: R2A with Sisomicin 0.035 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Sisomicin 0.035 mg/ml
3,292,013	-		RR42_RS15195	0.57	+0.9
3,292,075	+		RR42_RS15195	0.61	+0.4
3,292,075	+		RR42_RS15195	0.61	-0.2
3,292,076	-		RR42_RS15195	0.61	+1.0
3,292,159	+		RR42_RS15195	0.66	-1.2
3,292,159	+		RR42_RS15195	0.66	+1.7
3,292,159	+		RR42_RS15195	0.66	-0.1
3,292,159	+		RR42_RS15195	0.66	+0.3
3,292,159	+		RR42_RS15195	0.66	-0.4
3,292,160	-		RR42_RS15195	0.66	+2.3
3,292,160	-		RR42_RS15195	0.66	-0.9
3,292,160	-		RR42_RS15195	0.66	+0.1
3,292,213	+		RR42_RS15195	0.69	-0.1
3,292,213	+		RR42_RS15195	0.69	-0.0
3,292,213	+		RR42_RS15195	0.69	-0.8
3,292,213	+		RR42_RS15195	0.69	+0.6
3,292,278	-		RR42_RS15195	0.73	+0.0
3,292,453	+		RR42_RS15195	0.84	+1.0
3,292,454	-		RR42_RS15195	0.84	-0.9
3,292,454	-		RR42_RS15195	0.84	-0.4
3,292,600	+				+0.6
3,292,600	+				+0.1
3,292,627	-				-1.8
3,292,660	+				-0.3
3,292,660	+				-2.4
3,292,661	-				-1.0
3,292,661	-				+0.9
3,292,722	+				-0.0
3,292,722	+				+0.9
3,292,723	-				+0.5
3,292,723	-				+0.6
3,292,725	+				-0.6
3,292,725	+				+0.2
3,292,936	-				-0.2
3,292,936	-				+1.0
3,292,936	-				-1.1
3,292,983	+				+0.3
3,292,984	-				+0.6
3,292,984	-				-0.8
3,293,124	-		RR42_RS15200	0.17	+0.9
3,293,550	+		RR42_RS15200	0.67	+0.4
3,293,550	+		RR42_RS15200	0.67	+0.3
3,293,551	-		RR42_RS15200	0.67	-0.2
3,293,551	-		RR42_RS15200	0.67	-0.6
3,293,551	-		RR42_RS15200	0.67	+2.4
3,293,874	-				-1.7
3,294,048	+		RR42_RS15205	0.14	+1.2
3,294,049	-		RR42_RS15205	0.15	+0.4
3,294,063	+		RR42_RS15205	0.17	-0.2
3,294,064	-		RR42_RS15205	0.17	+0.2
3,294,120	+		RR42_RS15205	0.26	+0.9
3,294,573	+				-0.4
3,294,573	+				+1.2
3,294,573	+				+0.9
3,294,574	-				+2.8
3,294,574	-				-1.9
3,294,574	-				+0.5
3,294,586	+				+0.5
3,294,586	+				-0.2
3,294,586	+				+0.0
3,294,586	+				+1.2
3,294,586	+				-0.4
3,294,586	+				-0.8
3,294,586	+				-0.1
3,294,586	+				+0.1
3,294,587	-				+0.7
3,294,587	-				+0.7
3,294,587	-				+0.8
3,294,587	-				-0.1
3,294,587	-				+0.1
3,294,753	+		RR42_RS15210	0.28	-0.7
3,294,754	-		RR42_RS15210	0.28	-0.5
3,294,832	+		RR42_RS15210	0.40	-0.5
3,294,832	+		RR42_RS15210	0.40	+0.3
3,294,832	+		RR42_RS15210	0.40	-0.5
3,294,833	-		RR42_RS15210	0.40	-1.1