Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15080 and RR42_RS15085 are separated by 80 nucleotides RR42_RS15085 and RR42_RS15090 are separated by 16 nucleotides
RR42_RS15080: RR42_RS15080 - GntR family transcriptional regulator, at 3,261,806 to 3,262,573
_RS15080
RR42_RS15085: RR42_RS15085 - LacI family transcriptional regulator, at 3,262,654 to 3,263,631
_RS15085
RR42_RS15090: RR42_RS15090 - DNA helicase, at 3,263,648 to 3,266,011
_RS15090
Position (kb)
3262
3263
3264 Strain fitness (log2 ratio)
-1
0
1
2 at 3261.726 kb on + strand at 3261.981 kb on - strand, within RR42_RS15080 at 3262.358 kb on + strand, within RR42_RS15080 at 3262.359 kb on - strand, within RR42_RS15080 at 3262.634 kb on + strand at 3262.635 kb on - strand at 3262.764 kb on - strand, within RR42_RS15085 at 3262.772 kb on - strand, within RR42_RS15085 at 3262.923 kb on + strand, within RR42_RS15085 at 3262.923 kb on + strand, within RR42_RS15085 at 3262.924 kb on - strand, within RR42_RS15085 at 3262.924 kb on - strand, within RR42_RS15085 at 3262.969 kb on + strand, within RR42_RS15085 at 3262.969 kb on + strand, within RR42_RS15085 at 3262.969 kb on + strand, within RR42_RS15085 at 3262.970 kb on + strand, within RR42_RS15085 at 3263.029 kb on + strand, within RR42_RS15085 at 3263.030 kb on - strand, within RR42_RS15085 at 3263.122 kb on + strand, within RR42_RS15085 at 3263.123 kb on - strand, within RR42_RS15085 at 3263.123 kb on - strand, within RR42_RS15085 at 3263.209 kb on + strand, within RR42_RS15085 at 3263.209 kb on + strand, within RR42_RS15085 at 3263.209 kb on + strand, within RR42_RS15085 at 3263.209 kb on + strand, within RR42_RS15085 at 3263.210 kb on - strand, within RR42_RS15085 at 3263.395 kb on + strand, within RR42_RS15085 at 3263.396 kb on - strand, within RR42_RS15085 at 3263.396 kb on - strand, within RR42_RS15085 at 3263.396 kb on - strand, within RR42_RS15085 at 3263.404 kb on + strand, within RR42_RS15085 at 3263.839 kb on - strand at 3264.120 kb on + strand, within RR42_RS15090 at 3264.579 kb on + strand, within RR42_RS15090
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,261,726 + +0.6 3,261,981 - RR42_RS15080 0.23 +0.2 3,262,358 + RR42_RS15080 0.72 +0.2 3,262,359 - RR42_RS15080 0.72 -0.1 3,262,634 + +0.5 3,262,635 - +0.4 3,262,764 - RR42_RS15085 0.11 -0.6 3,262,772 - RR42_RS15085 0.12 +1.2 3,262,923 + RR42_RS15085 0.28 -0.4 3,262,923 + RR42_RS15085 0.28 +1.0 3,262,924 - RR42_RS15085 0.28 +0.4 3,262,924 - RR42_RS15085 0.28 -0.1 3,262,969 + RR42_RS15085 0.32 -0.9 3,262,969 + RR42_RS15085 0.32 -0.9 3,262,969 + RR42_RS15085 0.32 -0.6 3,262,970 + RR42_RS15085 0.32 -0.1 3,263,029 + RR42_RS15085 0.38 +1.2 3,263,030 - RR42_RS15085 0.38 +0.2 3,263,122 + RR42_RS15085 0.48 +0.8 3,263,123 - RR42_RS15085 0.48 +0.0 3,263,123 - RR42_RS15085 0.48 +0.4 3,263,209 + RR42_RS15085 0.57 +0.4 3,263,209 + RR42_RS15085 0.57 -0.9 3,263,209 + RR42_RS15085 0.57 +2.4 3,263,209 + RR42_RS15085 0.57 -1.2 3,263,210 - RR42_RS15085 0.57 +0.2 3,263,395 + RR42_RS15085 0.76 -0.2 3,263,396 - RR42_RS15085 0.76 -0.2 3,263,396 - RR42_RS15085 0.76 -0.9 3,263,396 - RR42_RS15085 0.76 +0.2 3,263,404 + RR42_RS15085 0.77 +0.3 3,263,839 - +1.2 3,264,120 + RR42_RS15090 0.20 -0.8 3,264,579 + RR42_RS15090 0.39 -1.1
Or see this region's nucleotide sequence