Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15025 and RR42_RS15030 are separated by 99 nucleotides RR42_RS15030 and RR42_RS15035 are separated by 30 nucleotides RR42_RS15035 and RR42_RS15040 are separated by 231 nucleotides
RR42_RS15025: RR42_RS15025 - membrane protein, at 3,249,001 to 3,249,921
_RS15025
RR42_RS15030: RR42_RS15030 - 3-mercaptopyruvate sulfurtransferase, at 3,250,021 to 3,250,878
_RS15030
RR42_RS15035: RR42_RS15035 - ZIP zinc transporter, at 3,250,909 to 3,251,685
_RS15035
RR42_RS15040: RR42_RS15040 - carboxymethylenebutenolidase, at 3,251,917 to 3,252,789
_RS15040
Position (kb)
3250
3251
3252 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3249.919 kb on + strand at 3249.920 kb on - strand at 3249.920 kb on - strand at 3249.920 kb on - strand at 3249.920 kb on - strand at 3249.999 kb on + strand at 3250.171 kb on + strand, within RR42_RS15030 at 3250.171 kb on + strand, within RR42_RS15030 at 3250.171 kb on + strand, within RR42_RS15030 at 3250.172 kb on - strand, within RR42_RS15030 at 3250.172 kb on - strand, within RR42_RS15030 at 3250.277 kb on + strand, within RR42_RS15030 at 3250.277 kb on + strand, within RR42_RS15030 at 3250.304 kb on - strand, within RR42_RS15030 at 3250.325 kb on - strand, within RR42_RS15030 at 3250.910 kb on + strand at 3250.910 kb on + strand at 3250.910 kb on + strand at 3250.911 kb on - strand at 3251.082 kb on + strand, within RR42_RS15035 at 3251.082 kb on + strand, within RR42_RS15035 at 3251.084 kb on + strand, within RR42_RS15035 at 3251.085 kb on - strand, within RR42_RS15035 at 3251.085 kb on - strand, within RR42_RS15035 at 3251.085 kb on - strand, within RR42_RS15035 at 3251.118 kb on - strand, within RR42_RS15035 at 3251.150 kb on + strand, within RR42_RS15035 at 3251.151 kb on - strand, within RR42_RS15035 at 3251.311 kb on - strand, within RR42_RS15035 at 3251.311 kb on - strand, within RR42_RS15035 at 3251.370 kb on + strand, within RR42_RS15035 at 3251.676 kb on + strand at 3251.714 kb on + strand at 3251.715 kb on - strand at 3251.821 kb on + strand at 3251.821 kb on + strand at 3251.821 kb on + strand at 3251.822 kb on - strand at 3251.825 kb on + strand at 3251.825 kb on + strand at 3251.826 kb on - strand at 3251.826 kb on - strand at 3251.996 kb on + strand at 3252.134 kb on + strand, within RR42_RS15040 at 3252.135 kb on - strand, within RR42_RS15040 at 3252.135 kb on - strand, within RR42_RS15040 at 3252.142 kb on + strand, within RR42_RS15040 at 3252.142 kb on + strand, within RR42_RS15040 at 3252.143 kb on - strand, within RR42_RS15040 at 3252.143 kb on - strand, within RR42_RS15040 at 3252.143 kb on - strand, within RR42_RS15040 at 3252.225 kb on - strand, within RR42_RS15040 at 3252.256 kb on - strand, within RR42_RS15040 at 3252.266 kb on + strand, within RR42_RS15040 at 3252.276 kb on + strand, within RR42_RS15040 at 3252.276 kb on + strand, within RR42_RS15040 at 3252.276 kb on + strand, within RR42_RS15040 at 3252.322 kb on - strand, within RR42_RS15040 at 3252.482 kb on + strand, within RR42_RS15040 at 3252.483 kb on - strand, within RR42_RS15040 at 3252.483 kb on - strand, within RR42_RS15040 at 3252.483 kb on - strand, within RR42_RS15040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,249,919 + +0.2 3,249,920 - +1.1 3,249,920 - +1.2 3,249,920 - +0.3 3,249,920 - -2.9 3,249,999 + +0.7 3,250,171 + RR42_RS15030 0.17 +0.4 3,250,171 + RR42_RS15030 0.17 -0.2 3,250,171 + RR42_RS15030 0.17 +2.0 3,250,172 - RR42_RS15030 0.18 +0.2 3,250,172 - RR42_RS15030 0.18 +0.2 3,250,277 + RR42_RS15030 0.30 -1.2 3,250,277 + RR42_RS15030 0.30 -0.2 3,250,304 - RR42_RS15030 0.33 +0.4 3,250,325 - RR42_RS15030 0.35 -0.1 3,250,910 + +2.2 3,250,910 + +0.4 3,250,910 + +0.5 3,250,911 - +0.4 3,251,082 + RR42_RS15035 0.22 -0.2 3,251,082 + RR42_RS15035 0.22 +0.4 3,251,084 + RR42_RS15035 0.23 -1.2 3,251,085 - RR42_RS15035 0.23 -0.1 3,251,085 - RR42_RS15035 0.23 -0.1 3,251,085 - RR42_RS15035 0.23 -2.3 3,251,118 - RR42_RS15035 0.27 -2.8 3,251,150 + RR42_RS15035 0.31 -0.2 3,251,151 - RR42_RS15035 0.31 -0.0 3,251,311 - RR42_RS15035 0.52 -1.2 3,251,311 - RR42_RS15035 0.52 -0.3 3,251,370 + RR42_RS15035 0.59 -1.0 3,251,676 + -1.5 3,251,714 + -0.2 3,251,715 - -0.8 3,251,821 + -0.8 3,251,821 + -0.0 3,251,821 + +0.1 3,251,822 - +0.5 3,251,825 + -1.4 3,251,825 + +0.0 3,251,826 - -1.3 3,251,826 - -0.4 3,251,996 + -1.2 3,252,134 + RR42_RS15040 0.25 +2.7 3,252,135 - RR42_RS15040 0.25 +0.2 3,252,135 - RR42_RS15040 0.25 -2.0 3,252,142 + RR42_RS15040 0.26 +0.0 3,252,142 + RR42_RS15040 0.26 +0.7 3,252,143 - RR42_RS15040 0.26 +0.1 3,252,143 - RR42_RS15040 0.26 +0.6 3,252,143 - RR42_RS15040 0.26 -0.5 3,252,225 - RR42_RS15040 0.35 +0.0 3,252,256 - RR42_RS15040 0.39 -1.0 3,252,266 + RR42_RS15040 0.40 -3.0 3,252,276 + RR42_RS15040 0.41 -0.3 3,252,276 + RR42_RS15040 0.41 -0.6 3,252,276 + RR42_RS15040 0.41 -0.2 3,252,322 - RR42_RS15040 0.46 -0.3 3,252,482 + RR42_RS15040 0.65 -1.3 3,252,483 - RR42_RS15040 0.65 +0.1 3,252,483 - RR42_RS15040 0.65 +0.2 3,252,483 - RR42_RS15040 0.65 +1.2
Or see this region's nucleotide sequence