Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14885 and RR42_RS14890 are separated by 0 nucleotides RR42_RS14890 and RR42_RS14895 are separated by 133 nucleotides RR42_RS14895 and RR42_RS14900 are separated by 7 nucleotides RR42_RS14900 and RR42_RS14905 are separated by 282 nucleotides
RR42_RS14885: RR42_RS14885 - DNA-directed RNA polymerase sigma-70 factor, at 3,220,088 to 3,220,612
_RS14885
RR42_RS14890: RR42_RS14890 - hypothetical protein, at 3,220,613 to 3,221,329
_RS14890
RR42_RS14895: RR42_RS14895 - peptidase, at 3,221,463 to 3,222,857
_RS14895
RR42_RS14900: RR42_RS14900 - hypothetical protein, at 3,222,865 to 3,223,284
_RS14900
RR42_RS14905: RR42_RS14905 - hypothetical protein, at 3,223,567 to 3,224,085
_RS14905
Position (kb)
3221
3222
3223 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3220.484 kb on - strand, within RR42_RS14885 at 3220.554 kb on + strand, within RR42_RS14885 at 3220.615 kb on - strand at 3220.845 kb on + strand, within RR42_RS14890 at 3220.846 kb on - strand, within RR42_RS14890 at 3220.861 kb on - strand, within RR42_RS14890 at 3220.930 kb on - strand, within RR42_RS14890 at 3221.157 kb on + strand, within RR42_RS14890 at 3221.158 kb on - strand, within RR42_RS14890 at 3221.283 kb on + strand at 3221.284 kb on - strand at 3221.284 kb on - strand at 3221.284 kb on - strand at 3221.407 kb on + strand at 3221.408 kb on - strand at 3222.178 kb on + strand, within RR42_RS14895 at 3222.178 kb on + strand, within RR42_RS14895 at 3222.178 kb on + strand, within RR42_RS14895 at 3222.280 kb on + strand, within RR42_RS14895 at 3222.562 kb on + strand, within RR42_RS14895 at 3222.562 kb on + strand, within RR42_RS14895 at 3222.562 kb on + strand, within RR42_RS14895 at 3223.387 kb on + strand at 3223.387 kb on + strand at 3223.388 kb on - strand at 3223.388 kb on - strand at 3223.393 kb on + strand at 3223.393 kb on + strand at 3223.393 kb on + strand at 3223.394 kb on - strand at 3223.394 kb on - strand at 3223.444 kb on + strand at 3223.445 kb on - strand at 3223.727 kb on + strand, within RR42_RS14905 at 3223.728 kb on - strand, within RR42_RS14905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 3,220,484 - RR42_RS14885 0.75 +0.6 3,220,554 + RR42_RS14885 0.89 +2.1 3,220,615 - -0.2 3,220,845 + RR42_RS14890 0.32 -0.7 3,220,846 - RR42_RS14890 0.32 -1.1 3,220,861 - RR42_RS14890 0.35 -0.2 3,220,930 - RR42_RS14890 0.44 -0.1 3,221,157 + RR42_RS14890 0.76 +0.9 3,221,158 - RR42_RS14890 0.76 -0.4 3,221,283 + -0.7 3,221,284 - -0.0 3,221,284 - +0.8 3,221,284 - +0.8 3,221,407 + +0.6 3,221,408 - -0.8 3,222,178 + RR42_RS14895 0.51 -0.7 3,222,178 + RR42_RS14895 0.51 -0.9 3,222,178 + RR42_RS14895 0.51 +1.4 3,222,280 + RR42_RS14895 0.59 +1.6 3,222,562 + RR42_RS14895 0.79 +0.8 3,222,562 + RR42_RS14895 0.79 +0.6 3,222,562 + RR42_RS14895 0.79 +1.2 3,223,387 + +1.6 3,223,387 + +0.9 3,223,388 - +1.6 3,223,388 - +0.6 3,223,393 + +1.3 3,223,393 + +0.6 3,223,393 + -0.0 3,223,394 - -0.5 3,223,394 - -2.0 3,223,444 + +0.1 3,223,445 - -0.3 3,223,727 + RR42_RS14905 0.31 -0.6 3,223,728 - RR42_RS14905 0.31 +0.4
Or see this region's nucleotide sequence