Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14750

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14740 and RR42_RS14745 overlap by 4 nucleotidesRR42_RS14745 and RR42_RS14750 are separated by 195 nucleotidesRR42_RS14750 and RR42_RS14755 are separated by 48 nucleotidesRR42_RS14755 and RR42_RS14760 are separated by 110 nucleotides RR42_RS14740: RR42_RS14740 - thymidylate synthase, at 3,177,476 to 3,178,270 _RS14740 RR42_RS14745: RR42_RS14745 - diacylglycerol kinase, at 3,178,267 to 3,178,767 _RS14745 RR42_RS14750: RR42_RS14750 - peptidase PMbA, at 3,178,963 to 3,180,333 _RS14750 RR42_RS14755: RR42_RS14755 - hypothetical protein, at 3,180,382 to 3,181,008 _RS14755 RR42_RS14760: RR42_RS14760 - molybdenum cofactor biosynthesis protein MogA, at 3,181,119 to 3,181,709 _RS14760 Position (kb) 3178 3179 3180 3181Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3178.784 kb on + strandat 3178.784 kb on + strandat 3178.964 kb on + strandat 3178.964 kb on + strandat 3178.964 kb on + strandat 3179.136 kb on - strand, within RR42_RS14750at 3179.172 kb on - strand, within RR42_RS14750at 3179.172 kb on - strand, within RR42_RS14750at 3179.172 kb on - strand, within RR42_RS14750at 3179.172 kb on - strand, within RR42_RS14750at 3179.172 kb on - strand, within RR42_RS14750at 3179.336 kb on + strand, within RR42_RS14750at 3179.336 kb on + strand, within RR42_RS14750at 3179.337 kb on - strand, within RR42_RS14750at 3179.351 kb on + strand, within RR42_RS14750at 3179.351 kb on + strand, within RR42_RS14750at 3179.351 kb on + strand, within RR42_RS14750at 3179.359 kb on + strand, within RR42_RS14750at 3179.360 kb on - strand, within RR42_RS14750at 3179.360 kb on - strand, within RR42_RS14750at 3179.360 kb on - strand, within RR42_RS14750at 3179.467 kb on - strand, within RR42_RS14750at 3179.467 kb on - strand, within RR42_RS14750at 3179.467 kb on - strand, within RR42_RS14750at 3179.467 kb on - strand, within RR42_RS14750at 3179.502 kb on - strand, within RR42_RS14750at 3179.657 kb on + strand, within RR42_RS14750at 3179.787 kb on - strand, within RR42_RS14750at 3179.848 kb on - strand, within RR42_RS14750at 3180.042 kb on - strand, within RR42_RS14750at 3180.042 kb on - strand, within RR42_RS14750at 3180.137 kb on - strand, within RR42_RS14750at 3180.137 kb on - strand, within RR42_RS14750at 3180.295 kb on - strandat 3180.296 kb on + strandat 3180.296 kb on + strandat 3180.296 kb on + strandat 3180.296 kb on + strandat 3180.297 kb on - strandat 3180.297 kb on - strandat 3180.297 kb on - strandat 3180.370 kb on + strandat 3180.370 kb on + strandat 3180.371 kb on - strandat 3180.371 kb on - strandat 3180.378 kb on + strandat 3180.508 kb on - strand, within RR42_RS14755at 3180.655 kb on + strand, within RR42_RS14755at 3180.655 kb on + strand, within RR42_RS14755at 3180.655 kb on + strand, within RR42_RS14755at 3180.655 kb on + strand, within RR42_RS14755at 3180.656 kb on - strand, within RR42_RS14755at 3180.934 kb on - strand, within RR42_RS14755at 3181.123 kb on + strandat 3181.124 kb on - strandat 3181.164 kb on + strandat 3181.165 kb on - strandat 3181.165 kb on - strandat 3181.165 kb on - strandat 3181.214 kb on + strand, within RR42_RS14760at 3181.215 kb on - strand, within RR42_RS14760

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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3,178,784 + -0.4
3,178,784 + +1.3
3,178,964 + +0.4
3,178,964 + -0.9
3,178,964 + -1.3
3,179,136 - RR42_RS14750 0.13 +0.6
3,179,172 - RR42_RS14750 0.15 -0.1
3,179,172 - RR42_RS14750 0.15 +0.1
3,179,172 - RR42_RS14750 0.15 +0.5
3,179,172 - RR42_RS14750 0.15 +0.3
3,179,172 - RR42_RS14750 0.15 -1.2
3,179,336 + RR42_RS14750 0.27 -1.2
3,179,336 + RR42_RS14750 0.27 +0.3
3,179,337 - RR42_RS14750 0.27 -0.2
3,179,351 + RR42_RS14750 0.28 -1.3
3,179,351 + RR42_RS14750 0.28 -2.0
3,179,351 + RR42_RS14750 0.28 -0.1
3,179,359 + RR42_RS14750 0.29 -0.8
3,179,360 - RR42_RS14750 0.29 -0.4
3,179,360 - RR42_RS14750 0.29 +0.5
3,179,360 - RR42_RS14750 0.29 +1.3
3,179,467 - RR42_RS14750 0.37 -0.1
3,179,467 - RR42_RS14750 0.37 +0.8
3,179,467 - RR42_RS14750 0.37 +0.2
3,179,467 - RR42_RS14750 0.37 +0.4
3,179,502 - RR42_RS14750 0.39 +1.0
3,179,657 + RR42_RS14750 0.51 -0.7
3,179,787 - RR42_RS14750 0.60 +0.5
3,179,848 - RR42_RS14750 0.65 +0.5
3,180,042 - RR42_RS14750 0.79 +0.8
3,180,042 - RR42_RS14750 0.79 +0.5
3,180,137 - RR42_RS14750 0.86 +1.4
3,180,137 - RR42_RS14750 0.86 +1.3
3,180,295 - +1.1
3,180,296 + -0.2
3,180,296 + +1.1
3,180,296 + +0.9
3,180,296 + +0.3
3,180,297 - -0.5
3,180,297 - +0.5
3,180,297 - +1.4
3,180,370 + -0.3
3,180,370 + +0.2
3,180,371 - -0.2
3,180,371 - +0.2
3,180,378 + -1.2
3,180,508 - RR42_RS14755 0.20 +0.5
3,180,655 + RR42_RS14755 0.44 +3.5
3,180,655 + RR42_RS14755 0.44 +1.4
3,180,655 + RR42_RS14755 0.44 +1.9
3,180,655 + RR42_RS14755 0.44 +0.3
3,180,656 - RR42_RS14755 0.44 +2.7
3,180,934 - RR42_RS14755 0.88 +0.1
3,181,123 + +2.3
3,181,124 - +0.2
3,181,164 + +1.1
3,181,165 - -0.7
3,181,165 - +0.7
3,181,165 - +0.3
3,181,214 + RR42_RS14760 0.16 +0.3
3,181,215 - RR42_RS14760 0.16 -0.5

Or see this region's nucleotide sequence