Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12965 and RR42_RS12970 are separated by 124 nucleotides RR42_RS12970 and RR42_RS12975 are separated by 176 nucleotides
RR42_RS12965: RR42_RS12965 - glycerol-3-phosphate transporter permease, at 2,790,069 to 2,790,950
_RS12965
RR42_RS12970: RR42_RS12970 - glycerol-3-phosphate ABC transporter substrate-binding protein, at 2,791,075 to 2,792,391
_RS12970
RR42_RS12975: RR42_RS12975 - haloacid dehalogenase, at 2,792,568 to 2,793,359
_RS12975
Position (kb)
2791
2792
2793 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2790.194 kb on - strand, within RR42_RS12965 at 2790.202 kb on + strand, within RR42_RS12965 at 2790.202 kb on + strand, within RR42_RS12965 at 2790.202 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.212 kb on + strand, within RR42_RS12965 at 2790.213 kb on - strand, within RR42_RS12965 at 2790.280 kb on + strand, within RR42_RS12965 at 2790.280 kb on + strand, within RR42_RS12965 at 2790.281 kb on - strand, within RR42_RS12965 at 2790.281 kb on - strand, within RR42_RS12965 at 2790.281 kb on - strand, within RR42_RS12965 at 2790.333 kb on + strand, within RR42_RS12965 at 2790.398 kb on + strand, within RR42_RS12965 at 2790.399 kb on - strand, within RR42_RS12965 at 2790.439 kb on + strand, within RR42_RS12965 at 2790.440 kb on - strand, within RR42_RS12965 at 2790.538 kb on + strand, within RR42_RS12965 at 2790.611 kb on - strand, within RR42_RS12965 at 2790.670 kb on + strand, within RR42_RS12965 at 2790.670 kb on + strand, within RR42_RS12965 at 2790.751 kb on + strand, within RR42_RS12965 at 2790.842 kb on - strand, within RR42_RS12965 at 2790.842 kb on - strand, within RR42_RS12965 at 2790.910 kb on + strand at 2790.911 kb on - strand at 2790.993 kb on + strand at 2790.993 kb on + strand at 2790.993 kb on + strand at 2790.994 kb on - strand at 2790.994 kb on - strand at 2790.997 kb on + strand at 2790.997 kb on + strand at 2790.998 kb on - strand at 2790.998 kb on - strand at 2791.077 kb on - strand at 2791.149 kb on + strand at 2791.149 kb on + strand at 2791.150 kb on - strand at 2791.150 kb on - strand at 2791.150 kb on - strand at 2791.200 kb on + strand at 2791.302 kb on - strand, within RR42_RS12970 at 2791.302 kb on - strand, within RR42_RS12970 at 2791.328 kb on + strand, within RR42_RS12970 at 2791.328 kb on + strand, within RR42_RS12970 at 2791.400 kb on + strand at 2791.400 kb on + strand, within RR42_RS12970 at 2791.400 kb on + strand, within RR42_RS12970 at 2791.427 kb on + strand, within RR42_RS12970 at 2791.427 kb on + strand, within RR42_RS12970 at 2791.428 kb on - strand, within RR42_RS12970 at 2791.428 kb on - strand, within RR42_RS12970 at 2791.514 kb on + strand, within RR42_RS12970 at 2791.515 kb on - strand, within RR42_RS12970 at 2791.612 kb on - strand, within RR42_RS12970 at 2791.612 kb on - strand, within RR42_RS12970 at 2791.647 kb on - strand, within RR42_RS12970 at 2791.655 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.689 kb on + strand, within RR42_RS12970 at 2791.827 kb on - strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.940 kb on + strand, within RR42_RS12970 at 2791.941 kb on - strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.943 kb on + strand, within RR42_RS12970 at 2791.944 kb on - strand, within RR42_RS12970 at 2791.944 kb on - strand, within RR42_RS12970 at 2791.962 kb on + strand, within RR42_RS12970 at 2791.962 kb on + strand, within RR42_RS12970 at 2791.962 kb on + strand, within RR42_RS12970 at 2791.962 kb on + strand, within RR42_RS12970 at 2791.962 kb on + strand, within RR42_RS12970 at 2791.962 kb on + strand, within RR42_RS12970 at 2791.963 kb on - strand, within RR42_RS12970 at 2791.963 kb on - strand, within RR42_RS12970 at 2791.963 kb on - strand, within RR42_RS12970 at 2792.003 kb on + strand, within RR42_RS12970 at 2792.003 kb on + strand, within RR42_RS12970 at 2792.003 kb on + strand, within RR42_RS12970 at 2792.004 kb on - strand, within RR42_RS12970 at 2792.004 kb on - strand, within RR42_RS12970 at 2792.006 kb on + strand, within RR42_RS12970 at 2792.006 kb on + strand, within RR42_RS12970 at 2792.007 kb on - strand, within RR42_RS12970 at 2792.027 kb on + strand, within RR42_RS12970 at 2792.028 kb on - strand, within RR42_RS12970 at 2792.028 kb on - strand, within RR42_RS12970 at 2792.028 kb on - strand, within RR42_RS12970 at 2792.028 kb on - strand, within RR42_RS12970 at 2792.028 kb on - strand, within RR42_RS12970 at 2792.109 kb on + strand, within RR42_RS12970 at 2792.128 kb on + strand, within RR42_RS12970 at 2792.129 kb on - strand, within RR42_RS12970 at 2792.208 kb on - strand, within RR42_RS12970 at 2792.208 kb on - strand, within RR42_RS12970 at 2792.208 kb on - strand, within RR42_RS12970 at 2792.208 kb on - strand, within RR42_RS12970 at 2792.208 kb on - strand, within RR42_RS12970 at 2792.226 kb on - strand, within RR42_RS12970 at 2792.226 kb on - strand, within RR42_RS12970 at 2792.384 kb on - strand at 2792.409 kb on + strand at 2792.409 kb on + strand at 2792.409 kb on + strand at 2792.409 kb on + strand at 2792.410 kb on - strand at 2792.410 kb on - strand at 2792.410 kb on - strand at 2792.443 kb on + strand at 2792.444 kb on - strand at 2792.471 kb on - strand at 2792.471 kb on - strand at 2792.509 kb on + strand at 2792.510 kb on - strand at 2792.522 kb on + strand at 2792.522 kb on + strand at 2792.554 kb on + strand at 2792.554 kb on + strand at 2792.555 kb on - strand at 2792.555 kb on - strand at 2792.620 kb on + strand at 2792.621 kb on - strand at 2792.676 kb on + strand, within RR42_RS12975 at 2792.676 kb on + strand, within RR42_RS12975 at 2792.676 kb on + strand, within RR42_RS12975 at 2792.676 kb on + strand, within RR42_RS12975 at 2792.677 kb on - strand, within RR42_RS12975 at 2792.677 kb on - strand, within RR42_RS12975 at 2792.677 kb on - strand, within RR42_RS12975 at 2792.767 kb on + strand, within RR42_RS12975 at 2792.809 kb on - strand, within RR42_RS12975 at 2793.288 kb on + strand at 2793.288 kb on + strand at 2793.288 kb on + strand at 2793.289 kb on - strand at 2793.290 kb on + strand at 2793.291 kb on - strand at 2793.291 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,790,194 - RR42_RS12965 0.14 -0.1 2,790,202 + RR42_RS12965 0.15 -0.6 2,790,202 + RR42_RS12965 0.15 +0.3 2,790,202 + RR42_RS12965 0.15 -0.5 2,790,212 + RR42_RS12965 0.16 -0.5 2,790,212 + RR42_RS12965 0.16 +0.0 2,790,212 + RR42_RS12965 0.16 -0.1 2,790,212 + RR42_RS12965 0.16 -1.3 2,790,212 + RR42_RS12965 0.16 +0.7 2,790,213 - RR42_RS12965 0.16 -0.5 2,790,280 + RR42_RS12965 0.24 +0.1 2,790,280 + RR42_RS12965 0.24 -0.1 2,790,281 - RR42_RS12965 0.24 -1.3 2,790,281 - RR42_RS12965 0.24 -0.2 2,790,281 - RR42_RS12965 0.24 +0.3 2,790,333 + RR42_RS12965 0.30 -0.4 2,790,398 + RR42_RS12965 0.37 +0.2 2,790,399 - RR42_RS12965 0.37 +0.2 2,790,439 + RR42_RS12965 0.42 +0.7 2,790,440 - RR42_RS12965 0.42 -1.4 2,790,538 + RR42_RS12965 0.53 +1.2 2,790,611 - RR42_RS12965 0.61 -0.1 2,790,670 + RR42_RS12965 0.68 -0.0 2,790,670 + RR42_RS12965 0.68 +0.7 2,790,751 + RR42_RS12965 0.77 +0.4 2,790,842 - RR42_RS12965 0.88 +0.4 2,790,842 - RR42_RS12965 0.88 -0.1 2,790,910 + -1.6 2,790,911 - +0.1 2,790,993 + +1.0 2,790,993 + +0.9 2,790,993 + -1.3 2,790,994 - -0.9 2,790,994 - -0.6 2,790,997 + +0.0 2,790,997 + +1.0 2,790,998 - -0.0 2,790,998 - +0.3 2,791,077 - -1.5 2,791,149 + +0.6 2,791,149 + -0.5 2,791,150 - +1.1 2,791,150 - -0.4 2,791,150 - -0.1 2,791,200 + +1.2 2,791,302 - RR42_RS12970 0.17 +1.6 2,791,302 - RR42_RS12970 0.17 +0.2 2,791,328 + RR42_RS12970 0.19 +0.1 2,791,328 + RR42_RS12970 0.19 -0.2 2,791,400 + +1.3 2,791,400 + RR42_RS12970 0.25 +3.1 2,791,400 + RR42_RS12970 0.25 +1.1 2,791,427 + RR42_RS12970 0.27 +1.8 2,791,427 + RR42_RS12970 0.27 +1.4 2,791,428 - RR42_RS12970 0.27 +0.2 2,791,428 - RR42_RS12970 0.27 -0.1 2,791,514 + RR42_RS12970 0.33 +1.6 2,791,515 - RR42_RS12970 0.33 -2.7 2,791,612 - RR42_RS12970 0.41 +0.1 2,791,612 - RR42_RS12970 0.41 +0.1 2,791,647 - RR42_RS12970 0.43 +1.7 2,791,655 + RR42_RS12970 0.44 -0.4 2,791,689 + RR42_RS12970 0.47 +0.2 2,791,689 + RR42_RS12970 0.47 +2.7 2,791,689 + RR42_RS12970 0.47 -0.5 2,791,689 + RR42_RS12970 0.47 +2.3 2,791,827 - RR42_RS12970 0.57 +0.1 2,791,940 + RR42_RS12970 0.66 -0.5 2,791,940 + RR42_RS12970 0.66 -0.5 2,791,940 + RR42_RS12970 0.66 -1.6 2,791,940 + RR42_RS12970 0.66 +1.3 2,791,941 - RR42_RS12970 0.66 +2.1 2,791,943 + RR42_RS12970 0.66 -1.2 2,791,943 + RR42_RS12970 0.66 -0.7 2,791,943 + RR42_RS12970 0.66 +0.5 2,791,943 + RR42_RS12970 0.66 +0.7 2,791,944 - RR42_RS12970 0.66 -0.2 2,791,944 - RR42_RS12970 0.66 +0.2 2,791,962 + RR42_RS12970 0.67 -1.3 2,791,962 + RR42_RS12970 0.67 -0.4 2,791,962 + RR42_RS12970 0.67 +1.5 2,791,962 + RR42_RS12970 0.67 +0.9 2,791,962 + RR42_RS12970 0.67 +1.1 2,791,962 + RR42_RS12970 0.67 +0.1 2,791,963 - RR42_RS12970 0.67 -0.7 2,791,963 - RR42_RS12970 0.67 +0.2 2,791,963 - RR42_RS12970 0.67 -0.8 2,792,003 + RR42_RS12970 0.70 +1.0 2,792,003 + RR42_RS12970 0.70 +0.5 2,792,003 + RR42_RS12970 0.70 -0.5 2,792,004 - RR42_RS12970 0.71 +1.2 2,792,004 - RR42_RS12970 0.71 +0.1 2,792,006 + RR42_RS12970 0.71 -0.5 2,792,006 + RR42_RS12970 0.71 +0.8 2,792,007 - RR42_RS12970 0.71 -0.3 2,792,027 + RR42_RS12970 0.72 -0.1 2,792,028 - RR42_RS12970 0.72 -0.2 2,792,028 - RR42_RS12970 0.72 +0.8 2,792,028 - RR42_RS12970 0.72 +2.3 2,792,028 - RR42_RS12970 0.72 +0.4 2,792,028 - RR42_RS12970 0.72 -0.4 2,792,109 + RR42_RS12970 0.79 -0.4 2,792,128 + RR42_RS12970 0.80 -0.6 2,792,129 - RR42_RS12970 0.80 +0.1 2,792,208 - RR42_RS12970 0.86 +0.9 2,792,208 - RR42_RS12970 0.86 -0.4 2,792,208 - RR42_RS12970 0.86 +1.3 2,792,208 - RR42_RS12970 0.86 +0.3 2,792,208 - RR42_RS12970 0.86 +1.5 2,792,226 - RR42_RS12970 0.87 -0.9 2,792,226 - RR42_RS12970 0.87 -1.2 2,792,384 - -0.6 2,792,409 + +1.8 2,792,409 + -0.9 2,792,409 + +0.7 2,792,409 + -1.1 2,792,410 - +0.2 2,792,410 - +0.7 2,792,410 - +0.8 2,792,443 + +0.8 2,792,444 - -1.2 2,792,471 - -0.1 2,792,471 - +0.3 2,792,509 + -1.1 2,792,510 - -1.1 2,792,522 + -2.1 2,792,522 + -1.2 2,792,554 + +0.4 2,792,554 + +0.2 2,792,555 - -2.2 2,792,555 - -2.2 2,792,620 + -0.1 2,792,621 - -3.0 2,792,676 + RR42_RS12975 0.14 -0.1 2,792,676 + RR42_RS12975 0.14 -0.1 2,792,676 + RR42_RS12975 0.14 +1.1 2,792,676 + RR42_RS12975 0.14 +0.1 2,792,677 - RR42_RS12975 0.14 -4.1 2,792,677 - RR42_RS12975 0.14 +0.1 2,792,677 - RR42_RS12975 0.14 -0.2 2,792,767 + RR42_RS12975 0.25 +0.3 2,792,809 - RR42_RS12975 0.30 -1.8 2,793,288 + +0.1 2,793,288 + -0.0 2,793,288 + -1.1 2,793,289 - +2.1 2,793,290 + -0.2 2,793,291 - -1.9 2,793,291 - -1.1
Or see this region's nucleotide sequence