Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12870 and RR42_RS12875 are separated by 298 nucleotides RR42_RS12875 and RR42_RS12880 are separated by 86 nucleotides RR42_RS12880 and RR42_RS12885 are separated by 2 nucleotides RR42_RS12885 and RR42_RS12890 are separated by 67 nucleotides
RR42_RS12870: RR42_RS12870 - membrane protein, at 2,767,858 to 2,768,622
_RS12870
RR42_RS12875: RR42_RS12875 - transcriptional regulator, at 2,768,921 to 2,769,646
_RS12875
RR42_RS12880: RR42_RS12880 - membrane protein, at 2,769,733 to 2,769,978
_RS12880
RR42_RS12885: RR42_RS12885 - membrane protein, at 2,769,981 to 2,770,199
_RS12885
RR42_RS12890: RR42_RS12890 - cytochrome C oxidase, at 2,770,267 to 2,771,766
_RS12890
Position (kb)
2768
2769
2770 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2768.283 kb on + strand, within RR42_RS12870 at 2768.429 kb on + strand, within RR42_RS12870 at 2768.430 kb on - strand, within RR42_RS12870 at 2768.430 kb on - strand, within RR42_RS12870 at 2768.430 kb on - strand, within RR42_RS12870 at 2768.430 kb on - strand, within RR42_RS12870 at 2768.605 kb on - strand at 2768.784 kb on + strand at 2768.784 kb on + strand at 2768.826 kb on - strand at 2769.031 kb on + strand, within RR42_RS12875 at 2769.032 kb on - strand, within RR42_RS12875 at 2769.509 kb on + strand, within RR42_RS12875 at 2769.776 kb on + strand, within RR42_RS12880 at 2769.776 kb on + strand, within RR42_RS12880 at 2769.777 kb on - strand, within RR42_RS12880 at 2769.777 kb on - strand, within RR42_RS12880 at 2769.836 kb on + strand, within RR42_RS12880 at 2769.971 kb on - strand at 2770.211 kb on + strand at 2770.212 kb on - strand at 2770.218 kb on - strand at 2770.226 kb on + strand at 2770.226 kb on + strand at 2770.227 kb on - strand at 2770.464 kb on - strand, within RR42_RS12890
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,768,283 + RR42_RS12870 0.56 +0.1 2,768,429 + RR42_RS12870 0.75 -0.1 2,768,430 - RR42_RS12870 0.75 -0.5 2,768,430 - RR42_RS12870 0.75 +0.6 2,768,430 - RR42_RS12870 0.75 +0.7 2,768,430 - RR42_RS12870 0.75 -0.7 2,768,605 - +0.9 2,768,784 + +1.1 2,768,784 + +1.7 2,768,826 - -0.3 2,769,031 + RR42_RS12875 0.15 -0.3 2,769,032 - RR42_RS12875 0.15 -2.3 2,769,509 + RR42_RS12875 0.81 -0.4 2,769,776 + RR42_RS12880 0.17 +0.1 2,769,776 + RR42_RS12880 0.17 -1.3 2,769,777 - RR42_RS12880 0.18 +1.1 2,769,777 - RR42_RS12880 0.18 +1.1 2,769,836 + RR42_RS12880 0.42 -0.3 2,769,971 - +0.5 2,770,211 + +2.1 2,770,212 - -0.8 2,770,218 - +0.3 2,770,226 + -0.5 2,770,226 + +0.9 2,770,227 - -0.5 2,770,464 - RR42_RS12890 0.13 +0.3
Or see this region's nucleotide sequence