Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10310 and RR42_RS10315 are separated by 2 nucleotides RR42_RS10315 and RR42_RS10320 are separated by 73 nucleotides RR42_RS10320 and RR42_RS10325 are separated by 306 nucleotides
RR42_RS10310: RR42_RS10310 - NADPH:quinone reductase, at 2,205,513 to 2,206,406
_RS10310
RR42_RS10315: RR42_RS10315 - short-chain dehydrogenase, at 2,206,409 to 2,207,170
_RS10315
RR42_RS10320: RR42_RS10320 - short-chain dehydrogenase, at 2,207,244 to 2,208,011
_RS10320
RR42_RS10325: RR42_RS10325 - 5-oxoprolinase, at 2,208,318 to 2,210,390
_RS10325
Position (kb)
2207
2208
2209 Strain fitness (log2 ratio)
-1
0
1 at 2206.539 kb on + strand, within RR42_RS10315 at 2206.539 kb on + strand, within RR42_RS10315 at 2206.548 kb on + strand, within RR42_RS10315 at 2206.696 kb on + strand, within RR42_RS10315 at 2206.696 kb on + strand, within RR42_RS10315 at 2206.696 kb on + strand, within RR42_RS10315 at 2206.729 kb on - strand, within RR42_RS10315 at 2206.935 kb on - strand, within RR42_RS10315 at 2207.081 kb on - strand, within RR42_RS10315 at 2207.185 kb on + strand at 2207.186 kb on - strand at 2207.186 kb on - strand at 2207.218 kb on - strand at 2207.450 kb on + strand, within RR42_RS10320 at 2207.451 kb on - strand, within RR42_RS10320 at 2207.451 kb on - strand, within RR42_RS10320 at 2207.484 kb on + strand, within RR42_RS10320 at 2207.548 kb on + strand, within RR42_RS10320 at 2207.549 kb on - strand, within RR42_RS10320 at 2207.557 kb on + strand, within RR42_RS10320 at 2207.558 kb on - strand, within RR42_RS10320 at 2207.558 kb on - strand, within RR42_RS10320 at 2207.558 kb on - strand, within RR42_RS10320 at 2207.675 kb on + strand, within RR42_RS10320 at 2207.676 kb on - strand, within RR42_RS10320 at 2207.895 kb on - strand, within RR42_RS10320 at 2207.895 kb on - strand, within RR42_RS10320 at 2207.989 kb on - strand at 2208.157 kb on + strand at 2208.180 kb on + strand at 2208.180 kb on + strand at 2208.317 kb on + strand at 2208.318 kb on - strand at 2208.335 kb on + strand at 2208.344 kb on + strand at 2208.345 kb on - strand at 2208.389 kb on + strand at 2208.389 kb on + strand at 2208.390 kb on - strand at 2208.390 kb on - strand at 2208.509 kb on + strand at 2208.510 kb on - strand at 2208.510 kb on - strand at 2208.513 kb on + strand at 2208.514 kb on - strand at 2208.514 kb on - strand at 2208.978 kb on + strand, within RR42_RS10325 at 2208.978 kb on + strand, within RR42_RS10325 at 2208.978 kb on + strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.979 kb on - strand, within RR42_RS10325 at 2208.980 kb on + strand, within RR42_RS10325 at 2208.980 kb on + strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325 at 2208.981 kb on - strand, within RR42_RS10325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,206,539 + RR42_RS10315 0.17 -0.1 2,206,539 + RR42_RS10315 0.17 -1.0 2,206,548 + RR42_RS10315 0.18 +0.3 2,206,696 + RR42_RS10315 0.38 +0.2 2,206,696 + RR42_RS10315 0.38 +0.4 2,206,696 + RR42_RS10315 0.38 -0.2 2,206,729 - RR42_RS10315 0.42 +0.1 2,206,935 - RR42_RS10315 0.69 +0.1 2,207,081 - RR42_RS10315 0.88 +0.7 2,207,185 + -0.9 2,207,186 - +0.5 2,207,186 - +0.9 2,207,218 - -0.1 2,207,450 + RR42_RS10320 0.27 -0.7 2,207,451 - RR42_RS10320 0.27 +0.7 2,207,451 - RR42_RS10320 0.27 +0.1 2,207,484 + RR42_RS10320 0.31 -1.7 2,207,548 + RR42_RS10320 0.40 -0.8 2,207,549 - RR42_RS10320 0.40 -1.1 2,207,557 + RR42_RS10320 0.41 -0.3 2,207,558 - RR42_RS10320 0.41 -1.5 2,207,558 - RR42_RS10320 0.41 -0.6 2,207,558 - RR42_RS10320 0.41 -0.6 2,207,675 + RR42_RS10320 0.56 -0.1 2,207,676 - RR42_RS10320 0.56 +1.3 2,207,895 - RR42_RS10320 0.85 -1.4 2,207,895 - RR42_RS10320 0.85 -0.6 2,207,989 - -0.5 2,208,157 + +0.4 2,208,180 + +1.3 2,208,180 + +0.5 2,208,317 + +0.9 2,208,318 - -0.9 2,208,335 + -0.6 2,208,344 + +0.2 2,208,345 - +0.4 2,208,389 + +0.5 2,208,389 + -0.3 2,208,390 - -0.1 2,208,390 - +0.3 2,208,509 + +0.4 2,208,510 - +0.3 2,208,510 - -1.5 2,208,513 + -1.2 2,208,514 - -1.0 2,208,514 - -0.5 2,208,978 + RR42_RS10325 0.32 +0.6 2,208,978 + RR42_RS10325 0.32 +0.1 2,208,978 + RR42_RS10325 0.32 -0.1 2,208,979 - RR42_RS10325 0.32 +0.2 2,208,979 - RR42_RS10325 0.32 +1.6 2,208,979 - RR42_RS10325 0.32 +1.6 2,208,979 - RR42_RS10325 0.32 -1.1 2,208,980 + RR42_RS10325 0.32 +0.8 2,208,980 + RR42_RS10325 0.32 -0.5 2,208,981 - RR42_RS10325 0.32 +0.1 2,208,981 - RR42_RS10325 0.32 +0.7 2,208,981 - RR42_RS10325 0.32 -0.1 2,208,981 - RR42_RS10325 0.32 -0.5 2,208,981 - RR42_RS10325 0.32 -0.5 2,208,981 - RR42_RS10325 0.32 -0.2
Or see this region's nucleotide sequence