Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10105 and RR42_RS10110 overlap by 4 nucleotides RR42_RS10110 and RR42_RS10115 are separated by 84 nucleotides RR42_RS10115 and RR42_RS10120 overlap by 2 nucleotides
RR42_RS10105: RR42_RS10105 - phosphonate ABC transporter, at 2,162,633 to 2,163,460
_RS10105
RR42_RS10110: RR42_RS10110 - phosphonate ABC transporter substrate-binding protein, at 2,163,457 to 2,164,320
_RS10110
RR42_RS10115: RR42_RS10115 - glycosyl transferase family 1, at 2,164,405 to 2,165,412
_RS10115
RR42_RS10120: RR42_RS10120 - glycosyl transferase, at 2,165,411 to 2,166,610
_RS10120
Position (kb)
2164
2165
2166 Strain fitness (log2 ratio)
-2
-1
0
1 at 2163.426 kb on + strand at 2163.659 kb on + strand, within RR42_RS10110 at 2163.662 kb on + strand, within RR42_RS10110 at 2163.663 kb on - strand, within RR42_RS10110 at 2163.976 kb on - strand, within RR42_RS10110 at 2163.976 kb on - strand, within RR42_RS10110 at 2164.047 kb on - strand, within RR42_RS10110 at 2164.047 kb on - strand, within RR42_RS10110 at 2164.047 kb on - strand, within RR42_RS10110 at 2164.112 kb on + strand, within RR42_RS10110 at 2164.164 kb on - strand, within RR42_RS10110 at 2164.320 kb on + strand at 2164.320 kb on + strand at 2164.321 kb on - strand at 2164.321 kb on - strand at 2164.321 kb on - strand at 2164.321 kb on - strand at 2164.411 kb on + strand at 2164.411 kb on + strand at 2164.411 kb on + strand at 2164.412 kb on - strand at 2164.412 kb on - strand at 2164.444 kb on + strand at 2164.445 kb on - strand at 2164.450 kb on - strand at 2164.592 kb on + strand, within RR42_RS10115 at 2164.593 kb on - strand, within RR42_RS10115 at 2164.601 kb on + strand, within RR42_RS10115 at 2164.601 kb on + strand, within RR42_RS10115 at 2164.602 kb on - strand, within RR42_RS10115 at 2164.602 kb on - strand, within RR42_RS10115 at 2164.602 kb on - strand, within RR42_RS10115 at 2164.602 kb on - strand, within RR42_RS10115 at 2164.677 kb on + strand, within RR42_RS10115 at 2164.678 kb on - strand, within RR42_RS10115 at 2164.781 kb on + strand, within RR42_RS10115 at 2164.789 kb on + strand, within RR42_RS10115 at 2164.795 kb on - strand, within RR42_RS10115 at 2164.835 kb on + strand, within RR42_RS10115 at 2164.835 kb on + strand, within RR42_RS10115 at 2164.917 kb on + strand, within RR42_RS10115 at 2164.917 kb on + strand, within RR42_RS10115 at 2164.918 kb on - strand, within RR42_RS10115 at 2164.918 kb on - strand, within RR42_RS10115 at 2165.018 kb on - strand, within RR42_RS10115 at 2165.018 kb on - strand, within RR42_RS10115 at 2165.075 kb on - strand, within RR42_RS10115 at 2165.075 kb on - strand, within RR42_RS10115 at 2165.075 kb on - strand, within RR42_RS10115 at 2165.382 kb on + strand at 2165.383 kb on - strand at 2165.383 kb on - strand at 2165.448 kb on + strand at 2165.576 kb on + strand, within RR42_RS10120 at 2165.577 kb on - strand, within RR42_RS10120 at 2165.577 kb on - strand, within RR42_RS10120 at 2165.577 kb on - strand, within RR42_RS10120 at 2165.597 kb on - strand, within RR42_RS10120 at 2165.611 kb on - strand, within RR42_RS10120 at 2165.667 kb on + strand, within RR42_RS10120 at 2165.667 kb on + strand, within RR42_RS10120 at 2165.754 kb on + strand, within RR42_RS10120 at 2165.755 kb on - strand, within RR42_RS10120 at 2165.755 kb on - strand, within RR42_RS10120 at 2165.755 kb on - strand, within RR42_RS10120 at 2165.762 kb on + strand, within RR42_RS10120 at 2165.831 kb on + strand, within RR42_RS10120 at 2165.885 kb on + strand, within RR42_RS10120 at 2165.886 kb on - strand, within RR42_RS10120 at 2165.886 kb on - strand, within RR42_RS10120 at 2166.064 kb on + strand, within RR42_RS10120 at 2166.064 kb on + strand, within RR42_RS10120 at 2166.064 kb on + strand, within RR42_RS10120 at 2166.172 kb on + strand, within RR42_RS10120 at 2166.173 kb on - strand, within RR42_RS10120 at 2166.173 kb on - strand, within RR42_RS10120 at 2166.314 kb on + strand, within RR42_RS10120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 2,163,426 + +0.6 2,163,659 + RR42_RS10110 0.23 +0.2 2,163,662 + RR42_RS10110 0.24 -1.1 2,163,663 - RR42_RS10110 0.24 -0.7 2,163,976 - RR42_RS10110 0.60 -2.2 2,163,976 - RR42_RS10110 0.60 +0.1 2,164,047 - RR42_RS10110 0.68 -2.1 2,164,047 - RR42_RS10110 0.68 -0.5 2,164,047 - RR42_RS10110 0.68 +1.3 2,164,112 + RR42_RS10110 0.76 +0.5 2,164,164 - RR42_RS10110 0.82 -0.8 2,164,320 + -0.9 2,164,320 + -0.0 2,164,321 - +0.6 2,164,321 - +0.1 2,164,321 - -0.4 2,164,321 - -0.3 2,164,411 + -1.8 2,164,411 + -0.7 2,164,411 + -0.5 2,164,412 - +0.6 2,164,412 - -0.0 2,164,444 + -0.4 2,164,445 - -0.1 2,164,450 - -0.8 2,164,592 + RR42_RS10115 0.19 -0.3 2,164,593 - RR42_RS10115 0.19 +0.1 2,164,601 + RR42_RS10115 0.19 +0.9 2,164,601 + RR42_RS10115 0.19 -1.7 2,164,602 - RR42_RS10115 0.20 -1.9 2,164,602 - RR42_RS10115 0.20 +0.3 2,164,602 - RR42_RS10115 0.20 +0.6 2,164,602 - RR42_RS10115 0.20 -1.1 2,164,677 + RR42_RS10115 0.27 -2.2 2,164,678 - RR42_RS10115 0.27 -0.6 2,164,781 + RR42_RS10115 0.37 -0.7 2,164,789 + RR42_RS10115 0.38 -0.6 2,164,795 - RR42_RS10115 0.39 -1.3 2,164,835 + RR42_RS10115 0.43 -2.1 2,164,835 + RR42_RS10115 0.43 +0.3 2,164,917 + RR42_RS10115 0.51 -1.2 2,164,917 + RR42_RS10115 0.51 +0.6 2,164,918 - RR42_RS10115 0.51 -0.3 2,164,918 - RR42_RS10115 0.51 -0.5 2,165,018 - RR42_RS10115 0.61 +0.5 2,165,018 - RR42_RS10115 0.61 +0.1 2,165,075 - RR42_RS10115 0.66 +0.4 2,165,075 - RR42_RS10115 0.66 +0.2 2,165,075 - RR42_RS10115 0.66 +0.3 2,165,382 + +0.0 2,165,383 - -0.1 2,165,383 - -0.1 2,165,448 + +1.1 2,165,576 + RR42_RS10120 0.14 +0.7 2,165,577 - RR42_RS10120 0.14 -0.8 2,165,577 - RR42_RS10120 0.14 -0.7 2,165,577 - RR42_RS10120 0.14 +1.2 2,165,597 - RR42_RS10120 0.15 -0.6 2,165,611 - RR42_RS10120 0.17 +0.1 2,165,667 + RR42_RS10120 0.21 -0.2 2,165,667 + RR42_RS10120 0.21 -1.4 2,165,754 + RR42_RS10120 0.29 -0.7 2,165,755 - RR42_RS10120 0.29 -0.2 2,165,755 - RR42_RS10120 0.29 -0.2 2,165,755 - RR42_RS10120 0.29 +0.1 2,165,762 + RR42_RS10120 0.29 +0.8 2,165,831 + RR42_RS10120 0.35 +0.4 2,165,885 + RR42_RS10120 0.40 +0.1 2,165,886 - RR42_RS10120 0.40 +0.6 2,165,886 - RR42_RS10120 0.40 +0.2 2,166,064 + RR42_RS10120 0.54 -0.7 2,166,064 + RR42_RS10120 0.54 +0.7 2,166,064 + RR42_RS10120 0.54 -0.9 2,166,172 + RR42_RS10120 0.63 +0.0 2,166,173 - RR42_RS10120 0.64 +1.2 2,166,173 - RR42_RS10120 0.64 +0.6 2,166,314 + RR42_RS10120 0.75 +0.1
Or see this region's nucleotide sequence