Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09005 and RR42_RS09010 are separated by 245 nucleotides RR42_RS09010 and RR42_RS09015 are separated by 226 nucleotides
RR42_RS09005: RR42_RS09005 - competence protein ComJ, at 1,890,826 to 1,891,683
_RS09005
RR42_RS09010: RR42_RS09010 - FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 1,891,929 to 1,893,953
_RS09010
RR42_RS09015: RR42_RS09015 - trigger factor, at 1,894,180 to 1,895,541
_RS09015
Position (kb)
1891
1892
1893
1894 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1891.094 kb on + strand, within RR42_RS09005 at 1891.094 kb on + strand, within RR42_RS09005 at 1891.094 kb on + strand, within RR42_RS09005 at 1891.094 kb on + strand, within RR42_RS09005 at 1891.095 kb on - strand, within RR42_RS09005 at 1891.095 kb on - strand, within RR42_RS09005 at 1891.141 kb on + strand, within RR42_RS09005 at 1891.151 kb on + strand, within RR42_RS09005 at 1891.152 kb on - strand, within RR42_RS09005 at 1891.152 kb on - strand, within RR42_RS09005 at 1891.169 kb on + strand, within RR42_RS09005 at 1891.169 kb on + strand, within RR42_RS09005 at 1891.170 kb on - strand, within RR42_RS09005 at 1891.170 kb on - strand, within RR42_RS09005 at 1891.170 kb on - strand, within RR42_RS09005 at 1891.251 kb on - strand, within RR42_RS09005 at 1891.251 kb on - strand, within RR42_RS09005 at 1891.251 kb on - strand, within RR42_RS09005 at 1891.430 kb on + strand, within RR42_RS09005 at 1891.542 kb on - strand, within RR42_RS09005 at 1891.542 kb on - strand, within RR42_RS09005 at 1891.572 kb on - strand, within RR42_RS09005 at 1891.572 kb on - strand, within RR42_RS09005 at 1891.757 kb on + strand at 1891.758 kb on - strand at 1891.758 kb on - strand at 1891.758 kb on - strand at 1891.942 kb on + strand at 1891.942 kb on + strand at 1891.942 kb on + strand at 1891.943 kb on - strand at 1891.984 kb on - strand at 1892.007 kb on + strand at 1892.045 kb on + strand at 1892.046 kb on - strand at 1892.046 kb on - strand at 1892.046 kb on - strand at 1892.046 kb on - strand at 1892.462 kb on + strand, within RR42_RS09010 at 1892.463 kb on - strand, within RR42_RS09010 at 1893.297 kb on + strand, within RR42_RS09010 at 1893.329 kb on + strand, within RR42_RS09010 at 1893.329 kb on + strand, within RR42_RS09010 at 1893.330 kb on - strand, within RR42_RS09010 at 1893.330 kb on - strand, within RR42_RS09010 at 1893.477 kb on + strand, within RR42_RS09010 at 1893.477 kb on + strand, within RR42_RS09010 at 1893.616 kb on - strand, within RR42_RS09010 at 1893.765 kb on + strand at 1893.765 kb on + strand at 1893.766 kb on - strand at 1893.766 kb on - strand at 1893.766 kb on - strand at 1894.633 kb on + strand, within RR42_RS09015 at 1894.633 kb on + strand, within RR42_RS09015
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,891,094 + RR42_RS09005 0.31 -0.0 1,891,094 + RR42_RS09005 0.31 +0.4 1,891,094 + RR42_RS09005 0.31 +0.2 1,891,094 + RR42_RS09005 0.31 -0.2 1,891,095 - RR42_RS09005 0.31 +0.4 1,891,095 - RR42_RS09005 0.31 -0.1 1,891,141 + RR42_RS09005 0.37 +1.4 1,891,151 + RR42_RS09005 0.38 +0.4 1,891,152 - RR42_RS09005 0.38 -0.3 1,891,152 - RR42_RS09005 0.38 +0.2 1,891,169 + RR42_RS09005 0.40 +0.0 1,891,169 + RR42_RS09005 0.40 +0.1 1,891,170 - RR42_RS09005 0.40 +0.5 1,891,170 - RR42_RS09005 0.40 +0.1 1,891,170 - RR42_RS09005 0.40 +0.4 1,891,251 - RR42_RS09005 0.50 -2.6 1,891,251 - RR42_RS09005 0.50 +1.2 1,891,251 - RR42_RS09005 0.50 -0.6 1,891,430 + RR42_RS09005 0.70 +0.7 1,891,542 - RR42_RS09005 0.83 +0.7 1,891,542 - RR42_RS09005 0.83 +0.3 1,891,572 - RR42_RS09005 0.87 +1.2 1,891,572 - RR42_RS09005 0.87 -0.7 1,891,757 + -0.1 1,891,758 - -1.6 1,891,758 - -0.2 1,891,758 - -0.0 1,891,942 + -0.2 1,891,942 + +0.7 1,891,942 + -0.6 1,891,943 - +0.3 1,891,984 - -0.5 1,892,007 + +0.3 1,892,045 + -1.9 1,892,046 - -1.8 1,892,046 - -0.3 1,892,046 - -0.2 1,892,046 - -0.1 1,892,462 + RR42_RS09010 0.26 -0.4 1,892,463 - RR42_RS09010 0.26 +0.2 1,893,297 + RR42_RS09010 0.68 +1.8 1,893,329 + RR42_RS09010 0.69 -0.3 1,893,329 + RR42_RS09010 0.69 +0.6 1,893,330 - RR42_RS09010 0.69 +1.9 1,893,330 - RR42_RS09010 0.69 -0.0 1,893,477 + RR42_RS09010 0.76 +1.1 1,893,477 + RR42_RS09010 0.76 +0.4 1,893,616 - RR42_RS09010 0.83 -0.0 1,893,765 + +0.7 1,893,765 + +1.6 1,893,766 - -1.3 1,893,766 - +0.0 1,893,766 - -2.3 1,894,633 + RR42_RS09015 0.33 -0.0 1,894,633 + RR42_RS09015 0.33 -2.0
Or see this region's nucleotide sequence