Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08745 and RR42_RS08750 are separated by 272 nucleotides RR42_RS08750 and RR42_RS08755 are separated by 874 nucleotides
RR42_RS08745: RR42_RS08745 - hypothetical protein, at 1,831,999 to 1,832,361
_RS08745
RR42_RS08750: RR42_RS08750 - hemerythrin, at 1,832,634 to 1,833,209
_RS08750
RR42_RS08755: RR42_RS08755 - molybdenum cofactor biosynthesis protein MoeA, at 1,834,084 to 1,835,208
_RS08755
Position (kb)
1832
1833
1834 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1831.744 kb on + strand at 1831.834 kb on + strand at 1831.834 kb on + strand at 1831.834 kb on + strand at 1831.907 kb on + strand at 1831.908 kb on - strand at 1831.908 kb on - strand at 1832.110 kb on + strand, within RR42_RS08745 at 1832.110 kb on + strand, within RR42_RS08745 at 1832.139 kb on + strand, within RR42_RS08745 at 1832.139 kb on + strand, within RR42_RS08745 at 1832.146 kb on - strand, within RR42_RS08745 at 1832.212 kb on + strand, within RR42_RS08745 at 1832.212 kb on + strand, within RR42_RS08745 at 1832.212 kb on + strand, within RR42_RS08745 at 1832.213 kb on - strand, within RR42_RS08745 at 1832.213 kb on - strand, within RR42_RS08745 at 1832.213 kb on - strand, within RR42_RS08745 at 1832.660 kb on - strand at 1832.833 kb on + strand, within RR42_RS08750 at 1832.834 kb on - strand, within RR42_RS08750 at 1833.056 kb on - strand, within RR42_RS08750 at 1833.058 kb on + strand, within RR42_RS08750 at 1833.059 kb on - strand, within RR42_RS08750 at 1833.173 kb on + strand at 1833.232 kb on + strand at 1833.233 kb on - strand at 1833.449 kb on + strand at 1833.450 kb on - strand at 1833.450 kb on - strand at 1833.450 kb on - strand at 1833.541 kb on + strand at 1833.542 kb on - strand at 1833.542 kb on - strand at 1833.560 kb on + strand at 1833.708 kb on + strand at 1833.709 kb on - strand at 1833.896 kb on + strand at 1834.049 kb on + strand at 1834.098 kb on - strand at 1834.098 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,831,744 + -0.0 1,831,834 + -0.2 1,831,834 + +0.3 1,831,834 + -0.8 1,831,907 + +0.3 1,831,908 - +0.9 1,831,908 - +0.1 1,832,110 + RR42_RS08745 0.31 +2.2 1,832,110 + RR42_RS08745 0.31 +0.6 1,832,139 + RR42_RS08745 0.39 +0.7 1,832,139 + RR42_RS08745 0.39 +0.2 1,832,146 - RR42_RS08745 0.40 +0.2 1,832,212 + RR42_RS08745 0.59 -0.4 1,832,212 + RR42_RS08745 0.59 +0.1 1,832,212 + RR42_RS08745 0.59 -0.6 1,832,213 - RR42_RS08745 0.59 -0.4 1,832,213 - RR42_RS08745 0.59 -0.4 1,832,213 - RR42_RS08745 0.59 -0.4 1,832,660 - +1.2 1,832,833 + RR42_RS08750 0.35 -1.2 1,832,834 - RR42_RS08750 0.35 +0.0 1,833,056 - RR42_RS08750 0.73 -0.6 1,833,058 + RR42_RS08750 0.74 -0.1 1,833,059 - RR42_RS08750 0.74 -1.4 1,833,173 + +0.2 1,833,232 + -0.1 1,833,233 - +0.4 1,833,449 + +1.0 1,833,450 - +0.9 1,833,450 - +0.3 1,833,450 - -0.3 1,833,541 + -2.4 1,833,542 - -1.3 1,833,542 - +0.4 1,833,560 + -0.5 1,833,708 + +0.3 1,833,709 - +1.7 1,833,896 + +0.0 1,834,049 + -0.3 1,834,098 - +0.3 1,834,098 - +0.0
Or see this region's nucleotide sequence