Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06985 and RR42_RS06990 are separated by 71 nucleotides RR42_RS06990 and RR42_RS06995 are separated by 13 nucleotides RR42_RS06995 and RR42_RS07000 are separated by 277 nucleotides RR42_RS07000 and RR42_RS07005 are separated by 291 nucleotides
RR42_RS06985: RR42_RS06985 - hypothetical protein, at 1,502,537 to 1,503,238
_RS06985
RR42_RS06990: RR42_RS06990 - hypothetical protein, at 1,503,310 to 1,503,570
_RS06990
RR42_RS06995: RR42_RS06995 - hypothetical protein, at 1,503,584 to 1,504,300
_RS06995
RR42_RS07000: RR42_RS07000 - hypothetical protein, at 1,504,578 to 1,504,781
_RS07000
RR42_RS07005: RR42_RS07005 - ATP synthase I, at 1,505,073 to 1,505,732
_RS07005
Position (kb)
1503
1504
1505 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1502.622 kb on + strand, within RR42_RS06985 at 1502.735 kb on + strand, within RR42_RS06985 at 1502.736 kb on - strand, within RR42_RS06985 at 1502.736 kb on - strand, within RR42_RS06985 at 1502.736 kb on - strand, within RR42_RS06985 at 1502.736 kb on - strand, within RR42_RS06985 at 1502.740 kb on + strand, within RR42_RS06985 at 1502.837 kb on + strand, within RR42_RS06985 at 1502.837 kb on + strand, within RR42_RS06985 at 1502.837 kb on + strand, within RR42_RS06985 at 1502.838 kb on - strand, within RR42_RS06985 at 1502.870 kb on + strand, within RR42_RS06985 at 1502.963 kb on + strand, within RR42_RS06985 at 1502.964 kb on - strand, within RR42_RS06985 at 1502.964 kb on - strand, within RR42_RS06985 at 1502.964 kb on - strand, within RR42_RS06985 at 1502.964 kb on - strand, within RR42_RS06985 at 1502.964 kb on - strand, within RR42_RS06985 at 1502.964 kb on - strand, within RR42_RS06985 at 1502.966 kb on + strand, within RR42_RS06985 at 1502.967 kb on - strand, within RR42_RS06985 at 1502.975 kb on + strand, within RR42_RS06985 at 1502.975 kb on + strand, within RR42_RS06985 at 1502.976 kb on - strand, within RR42_RS06985 at 1502.976 kb on - strand, within RR42_RS06985 at 1502.976 kb on - strand, within RR42_RS06985 at 1502.976 kb on - strand, within RR42_RS06985 at 1502.984 kb on + strand, within RR42_RS06985 at 1503.012 kb on - strand, within RR42_RS06985 at 1503.098 kb on + strand, within RR42_RS06985 at 1503.124 kb on + strand, within RR42_RS06985 at 1503.150 kb on + strand, within RR42_RS06985 at 1503.188 kb on + strand at 1503.191 kb on + strand at 1503.191 kb on + strand at 1503.191 kb on + strand at 1503.191 kb on + strand at 1503.192 kb on - strand at 1503.192 kb on - strand at 1503.192 kb on - strand at 1503.197 kb on + strand at 1503.197 kb on + strand at 1503.198 kb on - strand at 1503.250 kb on + strand at 1503.251 kb on - strand at 1503.305 kb on + strand at 1503.364 kb on + strand, within RR42_RS06990 at 1503.364 kb on + strand, within RR42_RS06990 at 1503.364 kb on + strand, within RR42_RS06990 at 1503.365 kb on - strand, within RR42_RS06990 at 1503.365 kb on - strand, within RR42_RS06990 at 1503.440 kb on + strand, within RR42_RS06990 at 1503.537 kb on - strand, within RR42_RS06990 at 1503.546 kb on - strand at 1503.551 kb on + strand at 1503.551 kb on + strand at 1503.551 kb on + strand at 1503.552 kb on - strand at 1503.552 kb on - strand at 1503.552 kb on - strand at 1503.585 kb on + strand at 1503.647 kb on + strand at 1503.648 kb on - strand at 1503.665 kb on + strand, within RR42_RS06995 at 1503.666 kb on - strand, within RR42_RS06995 at 1503.666 kb on - strand, within RR42_RS06995 at 1503.678 kb on - strand, within RR42_RS06995 at 1503.681 kb on - strand, within RR42_RS06995 at 1503.718 kb on + strand, within RR42_RS06995 at 1503.718 kb on + strand, within RR42_RS06995 at 1503.719 kb on - strand, within RR42_RS06995 at 1503.750 kb on + strand, within RR42_RS06995 at 1503.751 kb on - strand, within RR42_RS06995 at 1503.796 kb on - strand, within RR42_RS06995 at 1503.818 kb on + strand, within RR42_RS06995 at 1503.875 kb on + strand, within RR42_RS06995 at 1503.875 kb on + strand, within RR42_RS06995 at 1503.876 kb on - strand, within RR42_RS06995 at 1503.943 kb on + strand, within RR42_RS06995 at 1503.944 kb on - strand, within RR42_RS06995 at 1503.999 kb on - strand, within RR42_RS06995 at 1504.025 kb on + strand, within RR42_RS06995 at 1504.025 kb on + strand, within RR42_RS06995 at 1504.025 kb on + strand, within RR42_RS06995 at 1504.026 kb on - strand, within RR42_RS06995 at 1504.026 kb on - strand, within RR42_RS06995 at 1504.096 kb on + strand, within RR42_RS06995 at 1504.097 kb on - strand, within RR42_RS06995 at 1504.097 kb on - strand, within RR42_RS06995 at 1504.097 kb on - strand, within RR42_RS06995 at 1504.097 kb on - strand, within RR42_RS06995 at 1504.126 kb on + strand, within RR42_RS06995 at 1504.128 kb on + strand, within RR42_RS06995 at 1504.128 kb on + strand, within RR42_RS06995 at 1504.128 kb on + strand, within RR42_RS06995 at 1504.129 kb on - strand, within RR42_RS06995 at 1504.134 kb on + strand, within RR42_RS06995 at 1504.148 kb on + strand, within RR42_RS06995 at 1504.148 kb on + strand, within RR42_RS06995 at 1504.148 kb on + strand, within RR42_RS06995 at 1504.149 kb on - strand, within RR42_RS06995 at 1504.245 kb on - strand at 1504.278 kb on - strand at 1504.278 kb on - strand at 1504.307 kb on + strand at 1504.307 kb on + strand at 1504.308 kb on - strand at 1504.308 kb on - strand at 1504.310 kb on + strand at 1504.311 kb on - strand at 1504.317 kb on + strand at 1504.317 kb on + strand at 1504.443 kb on - strand at 1504.443 kb on - strand at 1504.475 kb on + strand at 1504.476 kb on - strand at 1504.476 kb on - strand at 1504.478 kb on - strand at 1504.490 kb on + strand at 1504.551 kb on + strand at 1504.552 kb on - strand at 1504.552 kb on - strand at 1504.552 kb on - strand at 1504.592 kb on + strand at 1504.592 kb on + strand at 1504.592 kb on + strand at 1504.593 kb on - strand at 1504.593 kb on - strand at 1504.664 kb on + strand, within RR42_RS07000 at 1504.681 kb on + strand, within RR42_RS07000 at 1504.681 kb on + strand, within RR42_RS07000 at 1504.681 kb on + strand, within RR42_RS07000 at 1504.681 kb on + strand, within RR42_RS07000 at 1504.682 kb on - strand, within RR42_RS07000 at 1504.682 kb on - strand, within RR42_RS07000 at 1504.682 kb on - strand, within RR42_RS07000 at 1504.771 kb on + strand at 1504.771 kb on + strand at 1504.771 kb on + strand at 1504.771 kb on + strand at 1504.772 kb on - strand at 1504.772 kb on - strand at 1504.772 kb on - strand at 1504.782 kb on - strand at 1504.820 kb on + strand at 1504.820 kb on + strand at 1504.820 kb on + strand at 1504.821 kb on - strand at 1504.940 kb on - strand at 1505.074 kb on + strand at 1505.074 kb on + strand at 1505.074 kb on + strand at 1505.074 kb on + strand at 1505.075 kb on - strand at 1505.138 kb on - strand at 1505.208 kb on + strand, within RR42_RS07005 at 1505.208 kb on + strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005 at 1505.209 kb on - strand, within RR42_RS07005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,502,622 + RR42_RS06985 0.12 -0.3 1,502,735 + RR42_RS06985 0.28 -0.2 1,502,736 - RR42_RS06985 0.28 -0.4 1,502,736 - RR42_RS06985 0.28 +0.1 1,502,736 - RR42_RS06985 0.28 +0.1 1,502,736 - RR42_RS06985 0.28 +0.3 1,502,740 + RR42_RS06985 0.29 -0.6 1,502,837 + RR42_RS06985 0.43 -1.1 1,502,837 + RR42_RS06985 0.43 +0.5 1,502,837 + RR42_RS06985 0.43 +0.0 1,502,838 - RR42_RS06985 0.43 -1.7 1,502,870 + RR42_RS06985 0.47 -0.3 1,502,963 + RR42_RS06985 0.61 +0.8 1,502,964 - RR42_RS06985 0.61 +0.1 1,502,964 - RR42_RS06985 0.61 -0.4 1,502,964 - RR42_RS06985 0.61 +0.7 1,502,964 - RR42_RS06985 0.61 +0.2 1,502,964 - RR42_RS06985 0.61 -0.1 1,502,964 - RR42_RS06985 0.61 -2.2 1,502,966 + RR42_RS06985 0.61 +0.6 1,502,967 - RR42_RS06985 0.61 +0.2 1,502,975 + RR42_RS06985 0.62 -0.4 1,502,975 + RR42_RS06985 0.62 +0.3 1,502,976 - RR42_RS06985 0.63 +0.1 1,502,976 - RR42_RS06985 0.63 +0.1 1,502,976 - RR42_RS06985 0.63 -0.8 1,502,976 - RR42_RS06985 0.63 -0.1 1,502,984 + RR42_RS06985 0.64 -1.9 1,503,012 - RR42_RS06985 0.68 -0.2 1,503,098 + RR42_RS06985 0.80 +0.2 1,503,124 + RR42_RS06985 0.84 -0.9 1,503,150 + RR42_RS06985 0.87 +0.0 1,503,188 + +0.2 1,503,191 + -0.3 1,503,191 + -0.8 1,503,191 + +0.3 1,503,191 + -0.3 1,503,192 - -2.8 1,503,192 - +0.3 1,503,192 - +1.0 1,503,197 + -0.3 1,503,197 + +0.1 1,503,198 - -0.1 1,503,250 + -0.1 1,503,251 - +2.0 1,503,305 + -0.9 1,503,364 + RR42_RS06990 0.21 -0.9 1,503,364 + RR42_RS06990 0.21 +0.3 1,503,364 + RR42_RS06990 0.21 +0.4 1,503,365 - RR42_RS06990 0.21 -0.9 1,503,365 - RR42_RS06990 0.21 +2.9 1,503,440 + RR42_RS06990 0.50 -0.3 1,503,537 - RR42_RS06990 0.87 +0.2 1,503,546 - -0.1 1,503,551 + +0.3 1,503,551 + -0.2 1,503,551 + -0.7 1,503,552 - -0.9 1,503,552 - -0.2 1,503,552 - -0.2 1,503,585 + -0.2 1,503,647 + +1.6 1,503,648 - +1.9 1,503,665 + RR42_RS06995 0.11 +0.3 1,503,666 - RR42_RS06995 0.11 +0.0 1,503,666 - RR42_RS06995 0.11 -0.3 1,503,678 - RR42_RS06995 0.13 +1.7 1,503,681 - RR42_RS06995 0.14 +0.7 1,503,718 + RR42_RS06995 0.19 +0.5 1,503,718 + RR42_RS06995 0.19 +0.2 1,503,719 - RR42_RS06995 0.19 -0.0 1,503,750 + RR42_RS06995 0.23 +0.5 1,503,751 - RR42_RS06995 0.23 -0.3 1,503,796 - RR42_RS06995 0.30 -0.3 1,503,818 + RR42_RS06995 0.33 +0.6 1,503,875 + RR42_RS06995 0.41 +0.4 1,503,875 + RR42_RS06995 0.41 -0.8 1,503,876 - RR42_RS06995 0.41 +0.6 1,503,943 + RR42_RS06995 0.50 +0.6 1,503,944 - RR42_RS06995 0.50 -0.3 1,503,999 - RR42_RS06995 0.58 -0.0 1,504,025 + RR42_RS06995 0.62 +0.1 1,504,025 + RR42_RS06995 0.62 -0.9 1,504,025 + RR42_RS06995 0.62 +0.7 1,504,026 - RR42_RS06995 0.62 -0.7 1,504,026 - RR42_RS06995 0.62 +0.3 1,504,096 + RR42_RS06995 0.71 -0.2 1,504,097 - RR42_RS06995 0.72 +0.6 1,504,097 - RR42_RS06995 0.72 +0.3 1,504,097 - RR42_RS06995 0.72 +1.5 1,504,097 - RR42_RS06995 0.72 +1.8 1,504,126 + RR42_RS06995 0.76 +1.7 1,504,128 + RR42_RS06995 0.76 +0.1 1,504,128 + RR42_RS06995 0.76 +0.4 1,504,128 + RR42_RS06995 0.76 +0.5 1,504,129 - RR42_RS06995 0.76 +1.7 1,504,134 + RR42_RS06995 0.77 -0.7 1,504,148 + RR42_RS06995 0.79 -0.9 1,504,148 + RR42_RS06995 0.79 +1.4 1,504,148 + RR42_RS06995 0.79 -0.5 1,504,149 - RR42_RS06995 0.79 +0.1 1,504,245 - +0.2 1,504,278 - +0.7 1,504,278 - +0.2 1,504,307 + +0.8 1,504,307 + +0.1 1,504,308 - -0.7 1,504,308 - +0.6 1,504,310 + +0.4 1,504,311 - -1.5 1,504,317 + +1.5 1,504,317 + -0.1 1,504,443 - -0.2 1,504,443 - -0.3 1,504,475 + +0.6 1,504,476 - +0.7 1,504,476 - +0.1 1,504,478 - +0.6 1,504,490 + +1.1 1,504,551 + +0.2 1,504,552 - -0.7 1,504,552 - -2.2 1,504,552 - +0.5 1,504,592 + +0.2 1,504,592 + -0.2 1,504,592 + +0.8 1,504,593 - -0.1 1,504,593 - +0.7 1,504,664 + RR42_RS07000 0.42 -0.2 1,504,681 + RR42_RS07000 0.50 -0.1 1,504,681 + RR42_RS07000 0.50 -0.7 1,504,681 + RR42_RS07000 0.50 -1.7 1,504,681 + RR42_RS07000 0.50 -0.3 1,504,682 - RR42_RS07000 0.51 +1.1 1,504,682 - RR42_RS07000 0.51 -0.3 1,504,682 - RR42_RS07000 0.51 -0.0 1,504,771 + -1.1 1,504,771 + -0.3 1,504,771 + +1.3 1,504,771 + -0.7 1,504,772 - -0.4 1,504,772 - -2.4 1,504,772 - -0.7 1,504,782 - -1.2 1,504,820 + +0.7 1,504,820 + +0.9 1,504,820 + -0.5 1,504,821 - +0.7 1,504,940 - -0.3 1,505,074 + -1.5 1,505,074 + +0.4 1,505,074 + -0.2 1,505,074 + +0.3 1,505,075 - -0.4 1,505,138 - +0.4 1,505,208 + RR42_RS07005 0.20 +0.0 1,505,208 + RR42_RS07005 0.20 +0.1 1,505,209 - RR42_RS07005 0.21 +0.3 1,505,209 - RR42_RS07005 0.21 -0.6 1,505,209 - RR42_RS07005 0.21 +1.1 1,505,209 - RR42_RS07005 0.21 +0.1
Or see this region's nucleotide sequence