Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06530 and RR42_RS06535 are separated by 69 nucleotides RR42_RS06535 and RR42_RS06540 are separated by 236 nucleotides
RR42_RS06530: RR42_RS06530 - C4-dicarboxylate ABC transporter, at 1,429,046 to 1,430,350
_RS06530
RR42_RS06535: RR42_RS06535 - C4-dicarboxylate ABC transporter, at 1,430,420 to 1,431,448
_RS06535
RR42_RS06540: RR42_RS06540 - hypothetical protein, at 1,431,685 to 1,432,389
_RS06540
Position (kb)
1430
1431
1432 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.538 kb on + strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.539 kb on - strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.540 kb on + strand, within RR42_RS06530 at 1429.657 kb on + strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.658 kb on - strand, within RR42_RS06530 at 1429.808 kb on + strand, within RR42_RS06530 at 1429.809 kb on - strand, within RR42_RS06530 at 1429.836 kb on - strand, within RR42_RS06530 at 1430.336 kb on + strand at 1430.336 kb on + strand at 1430.337 kb on - strand at 1430.362 kb on + strand at 1430.383 kb on + strand at 1430.455 kb on + strand at 1430.455 kb on + strand at 1430.455 kb on + strand at 1430.456 kb on - strand at 1430.504 kb on + strand at 1430.505 kb on - strand at 1430.505 kb on - strand at 1430.505 kb on - strand at 1430.777 kb on + strand, within RR42_RS06535 at 1430.777 kb on + strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.778 kb on - strand, within RR42_RS06535 at 1430.966 kb on + strand, within RR42_RS06535 at 1430.967 kb on - strand, within RR42_RS06535 at 1430.968 kb on + strand, within RR42_RS06535 at 1431.098 kb on + strand, within RR42_RS06535 at 1431.098 kb on + strand, within RR42_RS06535 at 1431.099 kb on - strand, within RR42_RS06535 at 1431.116 kb on + strand, within RR42_RS06535 at 1431.116 kb on + strand, within RR42_RS06535 at 1431.117 kb on - strand, within RR42_RS06535 at 1431.117 kb on - strand, within RR42_RS06535 at 1431.134 kb on + strand, within RR42_RS06535 at 1431.134 kb on + strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.135 kb on - strand, within RR42_RS06535 at 1431.299 kb on - strand, within RR42_RS06535 at 1431.378 kb on - strand at 1431.378 kb on - strand at 1431.378 kb on - strand at 1431.542 kb on + strand at 1431.574 kb on - strand at 1431.574 kb on - strand at 1431.574 kb on - strand at 1431.656 kb on + strand at 1431.916 kb on + strand, within RR42_RS06540 at 1431.916 kb on + strand, within RR42_RS06540 at 1431.916 kb on + strand, within RR42_RS06540 at 1431.916 kb on + strand, within RR42_RS06540 at 1431.970 kb on + strand, within RR42_RS06540 at 1431.970 kb on + strand, within RR42_RS06540 at 1431.970 kb on + strand, within RR42_RS06540 at 1431.970 kb on + strand, within RR42_RS06540 at 1431.971 kb on - strand, within RR42_RS06540 at 1431.985 kb on + strand, within RR42_RS06540 at 1432.045 kb on + strand, within RR42_RS06540 at 1432.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,429,538 + RR42_RS06530 0.38 -0.2 1,429,538 + RR42_RS06530 0.38 -0.1 1,429,538 + RR42_RS06530 0.38 +0.7 1,429,539 - RR42_RS06530 0.38 -0.1 1,429,539 - RR42_RS06530 0.38 -1.7 1,429,539 - RR42_RS06530 0.38 -1.1 1,429,539 - RR42_RS06530 0.38 +0.2 1,429,540 + RR42_RS06530 0.38 -0.2 1,429,540 + RR42_RS06530 0.38 +0.1 1,429,540 + RR42_RS06530 0.38 +0.1 1,429,657 + RR42_RS06530 0.47 -0.1 1,429,658 - RR42_RS06530 0.47 -0.4 1,429,658 - RR42_RS06530 0.47 -0.6 1,429,658 - RR42_RS06530 0.47 -0.5 1,429,808 + RR42_RS06530 0.58 +0.4 1,429,809 - RR42_RS06530 0.58 -0.0 1,429,836 - RR42_RS06530 0.61 -0.1 1,430,336 + +0.1 1,430,336 + -2.1 1,430,337 - +0.3 1,430,362 + -1.2 1,430,383 + -0.9 1,430,455 + +0.5 1,430,455 + +2.9 1,430,455 + -0.5 1,430,456 - +0.3 1,430,504 + +1.2 1,430,505 - +1.7 1,430,505 - -0.1 1,430,505 - +0.6 1,430,777 + RR42_RS06535 0.35 -0.7 1,430,777 + RR42_RS06535 0.35 -0.4 1,430,778 - RR42_RS06535 0.35 -0.6 1,430,778 - RR42_RS06535 0.35 -1.1 1,430,778 - RR42_RS06535 0.35 -0.5 1,430,966 + RR42_RS06535 0.53 -1.4 1,430,967 - RR42_RS06535 0.53 +1.3 1,430,968 + RR42_RS06535 0.53 -0.6 1,431,098 + RR42_RS06535 0.66 -0.4 1,431,098 + RR42_RS06535 0.66 -0.3 1,431,099 - RR42_RS06535 0.66 +0.5 1,431,116 + RR42_RS06535 0.68 +1.1 1,431,116 + RR42_RS06535 0.68 +0.1 1,431,117 - RR42_RS06535 0.68 +0.6 1,431,117 - RR42_RS06535 0.68 -1.0 1,431,134 + RR42_RS06535 0.69 +0.6 1,431,134 + RR42_RS06535 0.69 +1.7 1,431,135 - RR42_RS06535 0.69 -0.1 1,431,135 - RR42_RS06535 0.69 +0.9 1,431,135 - RR42_RS06535 0.69 -0.4 1,431,299 - RR42_RS06535 0.85 -1.4 1,431,378 - -0.4 1,431,378 - +0.8 1,431,378 - +0.9 1,431,542 + +0.0 1,431,574 - -1.1 1,431,574 - -0.2 1,431,574 - -0.2 1,431,656 + -1.1 1,431,916 + RR42_RS06540 0.33 -0.3 1,431,916 + RR42_RS06540 0.33 -0.3 1,431,916 + RR42_RS06540 0.33 -0.3 1,431,916 + RR42_RS06540 0.33 +1.4 1,431,970 + RR42_RS06540 0.40 +0.1 1,431,970 + RR42_RS06540 0.40 +1.3 1,431,970 + RR42_RS06540 0.40 +1.1 1,431,970 + RR42_RS06540 0.40 -0.0 1,431,971 - RR42_RS06540 0.41 +1.2 1,431,985 + RR42_RS06540 0.43 +2.7 1,432,045 + RR42_RS06540 0.51 +0.4 1,432,387 + +0.0
Or see this region's nucleotide sequence