Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06475 and RR42_RS06480 are separated by 22 nucleotides RR42_RS06480 and RR42_RS06485 are separated by 87 nucleotides RR42_RS06485 and RR42_RS06490 are separated by 1 nucleotides
RR42_RS06475: RR42_RS06475 - glycerol acyltransferase, at 1,416,381 to 1,418,279
_RS06475
RR42_RS06480: RR42_RS06480 - glutathione S-transferase, at 1,418,302 to 1,419,021
_RS06480
RR42_RS06485: RR42_RS06485 - chorismate synthase, at 1,419,109 to 1,420,227
_RS06485
RR42_RS06490: RR42_RS06490 - MFS transporter, at 1,420,229 to 1,421,455
_RS06490
Position (kb)
1419
1420
1421 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1418.314 kb on + strand at 1418.314 kb on + strand at 1418.314 kb on + strand at 1418.452 kb on + strand, within RR42_RS06480 at 1418.519 kb on - strand, within RR42_RS06480 at 1418.596 kb on - strand, within RR42_RS06480 at 1418.596 kb on - strand, within RR42_RS06480 at 1418.602 kb on + strand, within RR42_RS06480 at 1418.602 kb on + strand, within RR42_RS06480 at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.962 kb on + strand at 1418.963 kb on - strand at 1418.963 kb on - strand at 1418.989 kb on + strand at 1419.060 kb on + strand at 1419.061 kb on - strand at 1419.061 kb on - strand at 1419.067 kb on + strand at 1419.138 kb on + strand at 1419.138 kb on + strand at 1419.392 kb on - strand, within RR42_RS06485 at 1419.433 kb on + strand at 1419.433 kb on + strand, within RR42_RS06485 at 1419.440 kb on - strand, within RR42_RS06485 at 1419.440 kb on - strand, within RR42_RS06485 at 1419.451 kb on + strand, within RR42_RS06485 at 1419.451 kb on + strand, within RR42_RS06485 at 1419.553 kb on + strand, within RR42_RS06485 at 1419.576 kb on + strand, within RR42_RS06485 at 1419.576 kb on + strand, within RR42_RS06485 at 1419.576 kb on + strand, within RR42_RS06485 at 1419.576 kb on + strand, within RR42_RS06485 at 1419.576 kb on + strand, within RR42_RS06485 at 1419.577 kb on - strand, within RR42_RS06485 at 1419.577 kb on - strand, within RR42_RS06485 at 1419.682 kb on + strand, within RR42_RS06485 at 1419.682 kb on + strand, within RR42_RS06485 at 1419.682 kb on + strand, within RR42_RS06485 at 1420.301 kb on + strand at 1420.311 kb on - strand at 1420.340 kb on + strand at 1420.340 kb on + strand at 1420.340 kb on + strand at 1420.340 kb on + strand at 1420.340 kb on + strand at 1420.340 kb on + strand at 1420.341 kb on - strand at 1420.434 kb on - strand, within RR42_RS06490 at 1420.434 kb on - strand, within RR42_RS06490 at 1420.465 kb on + strand, within RR42_RS06490 at 1420.542 kb on - strand, within RR42_RS06490 at 1420.542 kb on - strand, within RR42_RS06490 at 1420.928 kb on + strand, within RR42_RS06490 at 1420.928 kb on + strand, within RR42_RS06490 at 1420.929 kb on - strand, within RR42_RS06490 at 1420.929 kb on - strand, within RR42_RS06490 at 1420.929 kb on - strand, within RR42_RS06490 at 1420.929 kb on - strand, within RR42_RS06490 at 1420.937 kb on + strand, within RR42_RS06490 at 1420.937 kb on + strand, within RR42_RS06490 at 1420.938 kb on - strand, within RR42_RS06490 at 1420.938 kb on - strand, within RR42_RS06490 at 1420.938 kb on - strand, within RR42_RS06490 at 1420.946 kb on + strand, within RR42_RS06490 at 1420.947 kb on - strand, within RR42_RS06490 at 1420.965 kb on - strand, within RR42_RS06490 at 1421.120 kb on + strand, within RR42_RS06490 at 1421.192 kb on - strand, within RR42_RS06490
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,418,314 + +0.9 1,418,314 + -0.6 1,418,314 + -0.4 1,418,452 + RR42_RS06480 0.21 -0.2 1,418,519 - RR42_RS06480 0.30 -0.8 1,418,596 - RR42_RS06480 0.41 +1.9 1,418,596 - RR42_RS06480 0.41 +1.5 1,418,602 + RR42_RS06480 0.42 -0.3 1,418,602 + RR42_RS06480 0.42 +0.1 1,418,962 + -0.6 1,418,962 + -0.6 1,418,962 + +0.7 1,418,962 + +0.9 1,418,962 + -0.6 1,418,963 - +0.1 1,418,963 - -1.9 1,418,989 + -0.9 1,419,060 + +0.7 1,419,061 - -0.1 1,419,061 - +1.5 1,419,067 + +0.4 1,419,138 + -0.8 1,419,138 + -0.4 1,419,392 - RR42_RS06485 0.25 -1.6 1,419,433 + -0.5 1,419,433 + RR42_RS06485 0.29 +0.0 1,419,440 - RR42_RS06485 0.30 +0.1 1,419,440 - RR42_RS06485 0.30 -0.7 1,419,451 + RR42_RS06485 0.31 -0.5 1,419,451 + RR42_RS06485 0.31 -0.3 1,419,553 + RR42_RS06485 0.40 -1.4 1,419,576 + RR42_RS06485 0.42 -0.0 1,419,576 + RR42_RS06485 0.42 -0.9 1,419,576 + RR42_RS06485 0.42 -2.2 1,419,576 + RR42_RS06485 0.42 -0.5 1,419,576 + RR42_RS06485 0.42 -2.2 1,419,577 - RR42_RS06485 0.42 -1.2 1,419,577 - RR42_RS06485 0.42 -0.9 1,419,682 + RR42_RS06485 0.51 -0.4 1,419,682 + RR42_RS06485 0.51 -1.0 1,419,682 + RR42_RS06485 0.51 -0.2 1,420,301 + +0.3 1,420,311 - +0.3 1,420,340 + +0.5 1,420,340 + +0.3 1,420,340 + +0.6 1,420,340 + -0.9 1,420,340 + -0.7 1,420,340 + -0.9 1,420,341 - -0.1 1,420,434 - RR42_RS06490 0.17 +0.4 1,420,434 - RR42_RS06490 0.17 +0.3 1,420,465 + RR42_RS06490 0.19 +0.1 1,420,542 - RR42_RS06490 0.26 +0.1 1,420,542 - RR42_RS06490 0.26 -1.1 1,420,928 + RR42_RS06490 0.57 +0.7 1,420,928 + RR42_RS06490 0.57 +0.7 1,420,929 - RR42_RS06490 0.57 -0.6 1,420,929 - RR42_RS06490 0.57 -0.3 1,420,929 - RR42_RS06490 0.57 +0.2 1,420,929 - RR42_RS06490 0.57 -0.1 1,420,937 + RR42_RS06490 0.58 -0.3 1,420,937 + RR42_RS06490 0.58 -1.2 1,420,938 - RR42_RS06490 0.58 +0.8 1,420,938 - RR42_RS06490 0.58 +0.0 1,420,938 - RR42_RS06490 0.58 +1.1 1,420,946 + RR42_RS06490 0.58 +0.4 1,420,947 - RR42_RS06490 0.59 +0.5 1,420,965 - RR42_RS06490 0.60 +0.4 1,421,120 + RR42_RS06490 0.73 -1.4 1,421,192 - RR42_RS06490 0.78 +0.1
Or see this region's nucleotide sequence