Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06410 and RR42_RS06415 are separated by 2 nucleotides RR42_RS06415 and RR42_RS06420 are separated by 341 nucleotides RR42_RS06420 and RR42_RS06425 are separated by 305 nucleotides RR42_RS06425 and RR42_RS06430 are separated by 85 nucleotides
RR42_RS06410: RR42_RS06410 - monooxygenase, at 1,404,659 to 1,405,882
_RS06410
RR42_RS06415: RR42_RS06415 - glyoxalase, at 1,405,885 to 1,406,373
_RS06415
RR42_RS06420: RR42_RS06420 - DSBA oxidoreductase, at 1,406,715 to 1,407,365
_RS06420
RR42_RS06425: RR42_RS06425 - anhydrase, at 1,407,671 to 1,408,213
_RS06425
RR42_RS06430: RR42_RS06430 - hypothetical protein, at 1,408,299 to 1,408,529
_RS06430
Position (kb)
1406
1407
1408 Strain fitness (log2 ratio)
-1
0
1
2 at 1405.778 kb on + strand at 1405.778 kb on + strand at 1405.779 kb on - strand at 1405.779 kb on - strand at 1406.240 kb on + strand, within RR42_RS06415 at 1406.240 kb on + strand, within RR42_RS06415 at 1406.240 kb on + strand, within RR42_RS06415 at 1406.386 kb on + strand at 1406.387 kb on - strand at 1406.395 kb on - strand at 1406.517 kb on + strand at 1406.518 kb on - strand at 1406.551 kb on + strand at 1406.559 kb on + strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.574 kb on - strand at 1406.682 kb on - strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.743 kb on - strand at 1406.835 kb on + strand, within RR42_RS06420 at 1406.836 kb on - strand, within RR42_RS06420 at 1406.985 kb on + strand, within RR42_RS06420 at 1406.986 kb on - strand, within RR42_RS06420 at 1406.986 kb on - strand, within RR42_RS06420 at 1407.103 kb on - strand, within RR42_RS06420 at 1407.139 kb on + strand, within RR42_RS06420 at 1407.185 kb on - strand, within RR42_RS06420 at 1407.185 kb on - strand, within RR42_RS06420 at 1407.185 kb on - strand, within RR42_RS06420 at 1407.367 kb on - strand at 1407.668 kb on - strand at 1407.756 kb on + strand, within RR42_RS06425 at 1407.963 kb on + strand, within RR42_RS06425 at 1407.964 kb on - strand, within RR42_RS06425 at 1408.088 kb on + strand, within RR42_RS06425 at 1408.088 kb on + strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.153 kb on + strand, within RR42_RS06425 at 1408.154 kb on - strand, within RR42_RS06425 at 1408.167 kb on - strand at 1408.239 kb on + strand at 1408.355 kb on + strand, within RR42_RS06430 at 1408.356 kb on - strand, within RR42_RS06430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,405,778 + +1.0 1,405,778 + +0.7 1,405,779 - -0.8 1,405,779 - +0.1 1,406,240 + RR42_RS06415 0.73 +1.7 1,406,240 + RR42_RS06415 0.73 -1.0 1,406,240 + RR42_RS06415 0.73 -0.2 1,406,386 + -0.2 1,406,387 - +0.2 1,406,395 - +0.5 1,406,517 + -0.1 1,406,518 - +0.2 1,406,551 + -0.5 1,406,559 + -0.5 1,406,560 - +0.1 1,406,560 - -0.8 1,406,560 - -0.8 1,406,560 - +0.1 1,406,560 - -0.5 1,406,574 - -0.9 1,406,682 - -1.3 1,406,742 + +0.1 1,406,742 + +0.1 1,406,742 + -0.2 1,406,742 + -0.7 1,406,742 + +2.1 1,406,743 - -0.2 1,406,835 + RR42_RS06420 0.18 +0.1 1,406,836 - RR42_RS06420 0.19 +1.3 1,406,985 + RR42_RS06420 0.41 +0.1 1,406,986 - RR42_RS06420 0.42 +0.7 1,406,986 - RR42_RS06420 0.42 +1.0 1,407,103 - RR42_RS06420 0.60 +1.2 1,407,139 + RR42_RS06420 0.65 +1.9 1,407,185 - RR42_RS06420 0.72 +0.9 1,407,185 - RR42_RS06420 0.72 +0.2 1,407,185 - RR42_RS06420 0.72 -0.7 1,407,367 - +0.5 1,407,668 - +0.9 1,407,756 + RR42_RS06425 0.16 -0.1 1,407,963 + RR42_RS06425 0.54 -0.6 1,407,964 - RR42_RS06425 0.54 +0.5 1,408,088 + RR42_RS06425 0.77 -0.7 1,408,088 + RR42_RS06425 0.77 +0.5 1,408,089 - RR42_RS06425 0.77 -0.0 1,408,089 - RR42_RS06425 0.77 -0.1 1,408,089 - RR42_RS06425 0.77 +0.4 1,408,089 - RR42_RS06425 0.77 -0.2 1,408,153 + RR42_RS06425 0.89 +0.3 1,408,154 - RR42_RS06425 0.89 -0.3 1,408,167 - +0.8 1,408,239 + -0.0 1,408,355 + RR42_RS06430 0.24 -1.2 1,408,356 - RR42_RS06430 0.25 -0.2
Or see this region's nucleotide sequence