Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05335 and RR42_RS05340 are separated by 12 nucleotides RR42_RS05340 and RR42_RS05345 are separated by 221 nucleotides
RR42_RS05335: RR42_RS05335 - aldehyde oxidase, at 1,179,015 to 1,181,372
_RS05335
RR42_RS05340: RR42_RS05340 - FAD-binding molybdopterin dehydrogenase, at 1,181,385 to 1,182,899
_RS05340
RR42_RS05345: RR42_RS05345 - LysR family transcriptional regulator, at 1,183,121 to 1,184,065
_RS05345
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.682 kb on + strand, within RR42_RS05335 at 1180.787 kb on - strand, within RR42_RS05335 at 1180.894 kb on + strand, within RR42_RS05335 at 1180.894 kb on + strand, within RR42_RS05335 at 1180.895 kb on - strand, within RR42_RS05335 at 1180.895 kb on - strand, within RR42_RS05335 at 1180.895 kb on - strand, within RR42_RS05335 at 1181.242 kb on + strand at 1181.242 kb on + strand at 1181.764 kb on + strand, within RR42_RS05340 at 1181.764 kb on + strand, within RR42_RS05340 at 1181.764 kb on + strand, within RR42_RS05340 at 1181.764 kb on + strand, within RR42_RS05340 at 1181.765 kb on - strand, within RR42_RS05340 at 1181.765 kb on - strand, within RR42_RS05340 at 1181.765 kb on - strand, within RR42_RS05340 at 1181.768 kb on + strand, within RR42_RS05340 at 1181.845 kb on + strand, within RR42_RS05340 at 1181.845 kb on + strand, within RR42_RS05340 at 1181.854 kb on + strand, within RR42_RS05340 at 1181.854 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.148 kb on + strand, within RR42_RS05340 at 1182.149 kb on - strand, within RR42_RS05340 at 1182.149 kb on - strand, within RR42_RS05340 at 1182.277 kb on + strand, within RR42_RS05340 at 1182.278 kb on - strand, within RR42_RS05340 at 1182.520 kb on + strand, within RR42_RS05340 at 1182.815 kb on - strand at 1182.875 kb on - strand at 1182.933 kb on + strand at 1182.933 kb on + strand at 1182.972 kb on + strand at 1182.973 kb on - strand at 1183.056 kb on + strand at 1183.056 kb on + strand at 1183.056 kb on + strand at 1183.056 kb on + strand at 1183.057 kb on - strand at 1183.057 kb on - strand at 1183.061 kb on + strand at 1183.061 kb on + strand at 1183.061 kb on + strand at 1183.062 kb on - strand at 1183.148 kb on + strand at 1183.148 kb on + strand at 1183.149 kb on - strand at 1183.149 kb on - strand at 1183.149 kb on - strand at 1183.240 kb on + strand, within RR42_RS05345 at 1183.240 kb on + strand, within RR42_RS05345 at 1183.240 kb on + strand, within RR42_RS05345 at 1183.466 kb on - strand, within RR42_RS05345 at 1183.613 kb on - strand, within RR42_RS05345 at 1183.697 kb on + strand, within RR42_RS05345 at 1183.730 kb on + strand, within RR42_RS05345 at 1183.730 kb on + strand, within RR42_RS05345 at 1183.731 kb on - strand, within RR42_RS05345 at 1183.781 kb on + strand, within RR42_RS05345 at 1183.781 kb on + strand, within RR42_RS05345 at 1183.781 kb on + strand, within RR42_RS05345 at 1183.781 kb on + strand, within RR42_RS05345 at 1183.782 kb on - strand, within RR42_RS05345 at 1183.782 kb on - strand, within RR42_RS05345 at 1183.829 kb on + strand, within RR42_RS05345 at 1183.829 kb on + strand, within RR42_RS05345 at 1183.829 kb on + strand, within RR42_RS05345 at 1183.830 kb on - strand, within RR42_RS05345 at 1183.849 kb on + strand, within RR42_RS05345 at 1183.886 kb on + strand, within RR42_RS05345 at 1183.896 kb on - strand, within RR42_RS05345 at 1183.896 kb on - strand, within RR42_RS05345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,180,682 + RR42_RS05335 0.71 -0.4 1,180,682 + RR42_RS05335 0.71 +0.0 1,180,682 + RR42_RS05335 0.71 -2.0 1,180,682 + RR42_RS05335 0.71 -1.0 1,180,682 + RR42_RS05335 0.71 -0.2 1,180,787 - RR42_RS05335 0.75 -0.1 1,180,894 + RR42_RS05335 0.80 -0.0 1,180,894 + RR42_RS05335 0.80 +0.1 1,180,895 - RR42_RS05335 0.80 +0.6 1,180,895 - RR42_RS05335 0.80 -0.9 1,180,895 - RR42_RS05335 0.80 +0.6 1,181,242 + -1.1 1,181,242 + +1.3 1,181,764 + RR42_RS05340 0.25 -1.4 1,181,764 + RR42_RS05340 0.25 -0.4 1,181,764 + RR42_RS05340 0.25 -1.4 1,181,764 + RR42_RS05340 0.25 -0.6 1,181,765 - RR42_RS05340 0.25 +0.2 1,181,765 - RR42_RS05340 0.25 -0.5 1,181,765 - RR42_RS05340 0.25 -0.2 1,181,768 + RR42_RS05340 0.25 +0.5 1,181,845 + RR42_RS05340 0.30 +0.3 1,181,845 + RR42_RS05340 0.30 -1.2 1,181,854 + RR42_RS05340 0.31 +0.6 1,181,854 + RR42_RS05340 0.31 +0.7 1,182,148 + RR42_RS05340 0.50 -0.4 1,182,148 + RR42_RS05340 0.50 -0.6 1,182,148 + RR42_RS05340 0.50 +0.5 1,182,148 + RR42_RS05340 0.50 -0.4 1,182,149 - RR42_RS05340 0.50 +0.3 1,182,149 - RR42_RS05340 0.50 +0.0 1,182,277 + RR42_RS05340 0.59 -0.2 1,182,278 - RR42_RS05340 0.59 +0.1 1,182,520 + RR42_RS05340 0.75 +0.3 1,182,815 - -1.0 1,182,875 - +1.1 1,182,933 + +1.7 1,182,933 + +0.1 1,182,972 + -0.1 1,182,973 - -0.4 1,183,056 + +0.8 1,183,056 + +0.3 1,183,056 + -0.4 1,183,056 + +0.9 1,183,057 - +0.7 1,183,057 - +0.6 1,183,061 + +0.1 1,183,061 + -0.2 1,183,061 + +1.1 1,183,062 - +0.9 1,183,148 + -1.2 1,183,148 + +0.1 1,183,149 - -0.2 1,183,149 - +0.7 1,183,149 - +0.3 1,183,240 + RR42_RS05345 0.13 +0.3 1,183,240 + RR42_RS05345 0.13 -0.7 1,183,240 + RR42_RS05345 0.13 +0.6 1,183,466 - RR42_RS05345 0.37 -0.4 1,183,613 - RR42_RS05345 0.52 -1.0 1,183,697 + RR42_RS05345 0.61 -0.5 1,183,730 + RR42_RS05345 0.64 +0.6 1,183,730 + RR42_RS05345 0.64 -1.0 1,183,731 - RR42_RS05345 0.65 -0.7 1,183,781 + RR42_RS05345 0.70 -1.8 1,183,781 + RR42_RS05345 0.70 +0.1 1,183,781 + RR42_RS05345 0.70 -0.1 1,183,781 + RR42_RS05345 0.70 +1.1 1,183,782 - RR42_RS05345 0.70 -1.3 1,183,782 - RR42_RS05345 0.70 +2.1 1,183,829 + RR42_RS05345 0.75 -0.3 1,183,829 + RR42_RS05345 0.75 +0.2 1,183,829 + RR42_RS05345 0.75 -0.7 1,183,830 - RR42_RS05345 0.75 +0.1 1,183,849 + RR42_RS05345 0.77 +0.6 1,183,886 + RR42_RS05345 0.81 -1.2 1,183,896 - RR42_RS05345 0.82 -0.5 1,183,896 - RR42_RS05345 0.82 +0.0
Or see this region's nucleotide sequence