Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05150 and RR42_RS05155 are separated by 172 nucleotides RR42_RS05155 and RR42_RS05160 are separated by 42 nucleotides RR42_RS05160 and RR42_RS05165 are separated by 248 nucleotides
RR42_RS05150: RR42_RS05150 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, at 1,135,076 to 1,136,014
_RS05150
RR42_RS05155: RR42_RS05155 - hydroxyacid dehydrogenase, at 1,136,187 to 1,137,137
_RS05155
RR42_RS05160: RR42_RS05160 - glyoxalase, at 1,137,180 to 1,137,653
_RS05160
RR42_RS05165: RR42_RS05165 - GntR family transcriptional regulator, at 1,137,902 to 1,138,567
_RS05165
Position (kb) 1136
1137
1138 Strain fitness (log2 ratio) -1
0
1
2 at 1135.292 kb on - strand, within RR42_RS05150 at 1135.292 kb on - strand, within RR42_RS05150 at 1135.399 kb on + strand, within RR42_RS05150 at 1135.440 kb on + strand, within RR42_RS05150 at 1135.489 kb on + strand, within RR42_RS05150 at 1135.492 kb on - strand, within RR42_RS05150 at 1135.653 kb on + strand, within RR42_RS05150 at 1135.654 kb on - strand, within RR42_RS05150 at 1135.654 kb on - strand, within RR42_RS05150 at 1135.761 kb on + strand, within RR42_RS05150 at 1135.762 kb on - strand, within RR42_RS05150 at 1135.938 kb on + strand at 1135.938 kb on + strand at 1135.938 kb on + strand at 1135.945 kb on + strand at 1135.989 kb on + strand at 1135.989 kb on + strand at 1135.989 kb on + strand at 1135.990 kb on - strand at 1136.218 kb on + strand at 1136.224 kb on + strand at 1136.421 kb on + strand, within RR42_RS05155 at 1136.489 kb on + strand, within RR42_RS05155 at 1136.490 kb on - strand, within RR42_RS05155 at 1136.648 kb on + strand, within RR42_RS05155 at 1136.649 kb on - strand, within RR42_RS05155 at 1136.701 kb on - strand, within RR42_RS05155 at 1136.897 kb on + strand, within RR42_RS05155 at 1136.897 kb on + strand, within RR42_RS05155 at 1136.898 kb on - strand, within RR42_RS05155 at 1137.181 kb on + strand at 1137.326 kb on - strand, within RR42_RS05160 at 1137.326 kb on - strand at 1137.326 kb on - strand, within RR42_RS05160 at 1137.326 kb on - strand, within RR42_RS05160 at 1137.326 kb on - strand, within RR42_RS05160 at 1137.326 kb on - strand, within RR42_RS05160 at 1137.361 kb on + strand, within RR42_RS05160 at 1137.361 kb on + strand, within RR42_RS05160 at 1137.361 kb on + strand, within RR42_RS05160 at 1137.361 kb on + strand, within RR42_RS05160 at 1137.361 kb on + strand, within RR42_RS05160 at 1137.361 kb on + strand, within RR42_RS05160 at 1137.362 kb on - strand, within RR42_RS05160 at 1137.362 kb on - strand, within RR42_RS05160 at 1137.362 kb on - strand, within RR42_RS05160 at 1137.362 kb on - strand, within RR42_RS05160 at 1137.362 kb on - strand, within RR42_RS05160 at 1137.526 kb on + strand, within RR42_RS05160 at 1137.589 kb on + strand, within RR42_RS05160 at 1137.590 kb on - strand, within RR42_RS05160 at 1137.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,135,292 - RR42_RS05150 0.23 -0.1 1,135,292 - RR42_RS05150 0.23 -0.2 1,135,399 + RR42_RS05150 0.34 +0.1 1,135,440 + RR42_RS05150 0.39 -1.0 1,135,489 + RR42_RS05150 0.44 -0.6 1,135,492 - RR42_RS05150 0.44 +1.1 1,135,653 + RR42_RS05150 0.61 +0.1 1,135,654 - RR42_RS05150 0.62 -0.1 1,135,654 - RR42_RS05150 0.62 +0.2 1,135,761 + RR42_RS05150 0.73 +0.1 1,135,762 - RR42_RS05150 0.73 +0.5 1,135,938 + -0.9 1,135,938 + -0.2 1,135,938 + -1.2 1,135,945 + -0.4 1,135,989 + +1.3 1,135,989 + +0.4 1,135,989 + -0.3 1,135,990 - +0.1 1,136,218 + -1.1 1,136,224 + -1.3 1,136,421 + RR42_RS05155 0.25 -0.1 1,136,489 + RR42_RS05155 0.32 -1.7 1,136,490 - RR42_RS05155 0.32 -0.2 1,136,648 + RR42_RS05155 0.48 -0.4 1,136,649 - RR42_RS05155 0.49 -0.4 1,136,701 - RR42_RS05155 0.54 -0.2 1,136,897 + RR42_RS05155 0.75 -0.6 1,136,897 + RR42_RS05155 0.75 -0.9 1,136,898 - RR42_RS05155 0.75 -0.0 1,137,181 + -0.9 1,137,326 - RR42_RS05160 0.31 +0.8 1,137,326 - +2.1 1,137,326 - RR42_RS05160 0.31 +0.1 1,137,326 - RR42_RS05160 0.31 +0.5 1,137,326 - RR42_RS05160 0.31 -0.9 1,137,326 - RR42_RS05160 0.31 +1.0 1,137,361 + RR42_RS05160 0.38 -0.5 1,137,361 + RR42_RS05160 0.38 -0.1 1,137,361 + RR42_RS05160 0.38 +0.2 1,137,361 + RR42_RS05160 0.38 +0.3 1,137,361 + RR42_RS05160 0.38 +0.2 1,137,361 + RR42_RS05160 0.38 -1.5 1,137,362 - RR42_RS05160 0.38 -0.6 1,137,362 - RR42_RS05160 0.38 +0.4 1,137,362 - RR42_RS05160 0.38 -0.5 1,137,362 - RR42_RS05160 0.38 +0.1 1,137,362 - RR42_RS05160 0.38 -0.0 1,137,526 + RR42_RS05160 0.73 +0.4 1,137,589 + RR42_RS05160 0.86 +0.1 1,137,590 - RR42_RS05160 0.86 -1.3 1,137,801 - +0.4
Or see this region's nucleotide sequence