Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS04985

Experiment: R2A with Sisomicin 0.035 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS04975 and RR42_RS04980 are separated by 206 nucleotidesRR42_RS04980 and RR42_RS04985 are separated by 172 nucleotidesRR42_RS04985 and RR42_RS04990 are separated by 174 nucleotidesRR42_RS04990 and RR42_RS04995 are separated by 31 nucleotides RR42_RS04975: RR42_RS04975 - histidine kinase, at 1,099,106 to 1,100,581 _RS04975 RR42_RS04980: RR42_RS04980 - hypothetical protein, at 1,100,788 to 1,101,189 _RS04980 RR42_RS04985: RR42_RS04985 - 2-aminoadipate aminotransferase, at 1,101,362 to 1,102,804 _RS04985 RR42_RS04990: RR42_RS04990 - hypothetical protein, at 1,102,979 to 1,103,344 _RS04990 RR42_RS04995: RR42_RS04995 - single-stranded DNA-binding protein, at 1,103,376 to 1,103,681 _RS04995 Position (kb) 1101 1102 1103Strain fitness (log2 ratio) -2 -1 0 1at 1100.544 kb on + strandat 1100.544 kb on + strandat 1100.544 kb on + strandat 1100.545 kb on - strandat 1100.716 kb on + strandat 1100.717 kb on - strandat 1100.717 kb on - strandat 1100.721 kb on + strandat 1100.740 kb on - strandat 1100.740 kb on - strandat 1100.745 kb on + strandat 1100.746 kb on - strandat 1101.000 kb on + strand, within RR42_RS04980at 1101.001 kb on - strand, within RR42_RS04980at 1101.768 kb on + strand, within RR42_RS04985at 1101.768 kb on + strand, within RR42_RS04985at 1101.769 kb on - strand, within RR42_RS04985at 1102.060 kb on + strand, within RR42_RS04985at 1102.068 kb on + strand, within RR42_RS04985at 1102.069 kb on - strand, within RR42_RS04985at 1102.069 kb on - strand, within RR42_RS04985at 1102.069 kb on - strand, within RR42_RS04985at 1102.176 kb on + strand, within RR42_RS04985at 1102.176 kb on + strand, within RR42_RS04985at 1102.177 kb on - strand, within RR42_RS04985at 1102.177 kb on - strand, within RR42_RS04985at 1102.177 kb on - strand, within RR42_RS04985at 1102.179 kb on + strand, within RR42_RS04985at 1102.179 kb on + strand, within RR42_RS04985at 1102.180 kb on - strand, within RR42_RS04985at 1102.180 kb on - strand, within RR42_RS04985at 1102.180 kb on - strand, within RR42_RS04985at 1102.273 kb on + strand, within RR42_RS04985at 1102.274 kb on - strand, within RR42_RS04985at 1102.527 kb on + strand, within RR42_RS04985at 1102.527 kb on + strand, within RR42_RS04985at 1102.527 kb on + strand, within RR42_RS04985at 1102.528 kb on - strand, within RR42_RS04985at 1102.764 kb on + strandat 1102.764 kb on + strandat 1102.841 kb on + strandat 1102.841 kb on + strandat 1102.881 kb on - strandat 1102.942 kb on + strandat 1102.942 kb on + strandat 1102.988 kb on + strandat 1102.989 kb on - strandat 1102.989 kb on - strandat 1103.125 kb on + strand, within RR42_RS04990at 1103.608 kb on + strand, within RR42_RS04995at 1103.608 kb on + strand, within RR42_RS04995at 1103.609 kb on - strand, within RR42_RS04995at 1103.609 kb on - strand, within RR42_RS04995at 1103.609 kb on - strand, within RR42_RS04995at 1103.609 kb on - strand, within RR42_RS04995

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml
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1,100,544 + +0.5
1,100,544 + +0.5
1,100,544 + +0.2
1,100,545 - -0.6
1,100,716 + -0.1
1,100,717 - +0.2
1,100,717 - +1.6
1,100,721 + -0.3
1,100,740 - +0.4
1,100,740 - -1.0
1,100,745 + +0.6
1,100,746 - +1.0
1,101,000 + RR42_RS04980 0.53 -0.4
1,101,001 - RR42_RS04980 0.53 -1.6
1,101,768 + RR42_RS04985 0.28 -2.0
1,101,768 + RR42_RS04985 0.28 -0.1
1,101,769 - RR42_RS04985 0.28 +0.6
1,102,060 + RR42_RS04985 0.48 +0.5
1,102,068 + RR42_RS04985 0.49 +0.2
1,102,069 - RR42_RS04985 0.49 -0.2
1,102,069 - RR42_RS04985 0.49 +1.1
1,102,069 - RR42_RS04985 0.49 +0.6
1,102,176 + RR42_RS04985 0.56 -1.0
1,102,176 + RR42_RS04985 0.56 +0.2
1,102,177 - RR42_RS04985 0.56 +1.0
1,102,177 - RR42_RS04985 0.56 +0.9
1,102,177 - RR42_RS04985 0.56 +0.7
1,102,179 + RR42_RS04985 0.57 -0.2
1,102,179 + RR42_RS04985 0.57 -0.5
1,102,180 - RR42_RS04985 0.57 +0.0
1,102,180 - RR42_RS04985 0.57 -0.8
1,102,180 - RR42_RS04985 0.57 -1.2
1,102,273 + RR42_RS04985 0.63 +0.9
1,102,274 - RR42_RS04985 0.63 +0.3
1,102,527 + RR42_RS04985 0.81 +0.4
1,102,527 + RR42_RS04985 0.81 -0.4
1,102,527 + RR42_RS04985 0.81 +0.2
1,102,528 - RR42_RS04985 0.81 +1.0
1,102,764 + +0.4
1,102,764 + +0.4
1,102,841 + +1.1
1,102,841 + -1.2
1,102,881 - +0.7
1,102,942 + +0.5
1,102,942 + -2.0
1,102,988 + +0.9
1,102,989 - +0.9
1,102,989 - +0.2
1,103,125 + RR42_RS04990 0.40 +0.2
1,103,608 + RR42_RS04995 0.76 +0.6
1,103,608 + RR42_RS04995 0.76 -1.8
1,103,609 - RR42_RS04995 0.76 -0.8
1,103,609 - RR42_RS04995 0.76 +0.7
1,103,609 - RR42_RS04995 0.76 -1.2
1,103,609 - RR42_RS04995 0.76 -0.4

Or see this region's nucleotide sequence