Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04920 and RR42_RS04925 are separated by 44 nucleotides RR42_RS04925 and RR42_RS04930 are separated by 123 nucleotides RR42_RS04930 and RR42_RS04935 are separated by 274 nucleotides
RR42_RS04920: RR42_RS04920 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 1,090,027 to 1,092,444
_RS04920
RR42_RS04925: RR42_RS04925 - transcription elongation factor GreB, at 1,092,489 to 1,093,085
_RS04925
RR42_RS04930: RR42_RS04930 - tRNA-Arg, at 1,093,209 to 1,093,285
_RS04930
RR42_RS04935: RR42_RS04935 - hypothetical protein, at 1,093,560 to 1,094,417
_RS04935
Position (kb)
1092
1093
1094 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1092.119 kb on + strand, within RR42_RS04920 at 1092.331 kb on + strand at 1092.331 kb on + strand at 1092.332 kb on - strand at 1092.332 kb on - strand at 1092.557 kb on + strand, within RR42_RS04925 at 1092.600 kb on - strand, within RR42_RS04925 at 1092.727 kb on + strand, within RR42_RS04925 at 1092.727 kb on + strand, within RR42_RS04925 at 1092.728 kb on - strand, within RR42_RS04925 at 1092.728 kb on - strand, within RR42_RS04925 at 1092.859 kb on + strand, within RR42_RS04925 at 1092.860 kb on - strand, within RR42_RS04925 at 1092.865 kb on + strand, within RR42_RS04925 at 1092.866 kb on - strand, within RR42_RS04925 at 1092.976 kb on + strand, within RR42_RS04925 at 1092.976 kb on + strand, within RR42_RS04925 at 1092.994 kb on + strand, within RR42_RS04925 at 1092.994 kb on + strand, within RR42_RS04925 at 1092.994 kb on + strand, within RR42_RS04925 at 1092.995 kb on - strand, within RR42_RS04925 at 1092.995 kb on - strand, within RR42_RS04925 at 1093.114 kb on + strand at 1093.115 kb on - strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.116 kb on + strand at 1093.117 kb on - strand at 1093.117 kb on - strand at 1093.117 kb on - strand at 1093.117 kb on - strand at 1093.193 kb on - strand at 1093.193 kb on - strand at 1093.373 kb on - strand at 1093.449 kb on - strand at 1093.455 kb on + strand at 1093.456 kb on - strand at 1093.456 kb on - strand at 1093.759 kb on - strand, within RR42_RS04935 at 1093.877 kb on + strand, within RR42_RS04935 at 1093.877 kb on + strand, within RR42_RS04935 at 1093.877 kb on + strand, within RR42_RS04935 at 1093.878 kb on - strand, within RR42_RS04935 at 1093.878 kb on - strand, within RR42_RS04935 at 1093.878 kb on - strand, within RR42_RS04935 at 1093.884 kb on - strand, within RR42_RS04935 at 1093.945 kb on - strand, within RR42_RS04935
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 1,092,119 + RR42_RS04920 0.87 +1.2 1,092,331 + +1.2 1,092,331 + -0.5 1,092,332 - +0.6 1,092,332 - +0.4 1,092,557 + RR42_RS04925 0.11 +1.2 1,092,600 - RR42_RS04925 0.19 +1.1 1,092,727 + RR42_RS04925 0.40 +0.2 1,092,727 + RR42_RS04925 0.40 +0.4 1,092,728 - RR42_RS04925 0.40 +0.2 1,092,728 - RR42_RS04925 0.40 -0.2 1,092,859 + RR42_RS04925 0.62 +0.5 1,092,860 - RR42_RS04925 0.62 -0.7 1,092,865 + RR42_RS04925 0.63 -0.1 1,092,866 - RR42_RS04925 0.63 +0.5 1,092,976 + RR42_RS04925 0.82 +0.3 1,092,976 + RR42_RS04925 0.82 -0.6 1,092,994 + RR42_RS04925 0.85 +0.4 1,092,994 + RR42_RS04925 0.85 +0.4 1,092,994 + RR42_RS04925 0.85 -0.1 1,092,995 - RR42_RS04925 0.85 +0.4 1,092,995 - RR42_RS04925 0.85 +0.2 1,093,114 + -0.4 1,093,115 - -0.8 1,093,116 + +1.5 1,093,116 + +0.3 1,093,116 + -0.3 1,093,116 + +1.4 1,093,116 + -0.3 1,093,117 - -0.5 1,093,117 - -0.7 1,093,117 - -2.0 1,093,117 - -1.1 1,093,193 - +0.6 1,093,193 - +0.5 1,093,373 - -0.4 1,093,449 - -0.8 1,093,455 + +0.4 1,093,456 - +0.6 1,093,456 - -0.3 1,093,759 - RR42_RS04935 0.23 +0.1 1,093,877 + RR42_RS04935 0.37 +0.7 1,093,877 + RR42_RS04935 0.37 -0.1 1,093,877 + RR42_RS04935 0.37 +1.8 1,093,878 - RR42_RS04935 0.37 +0.2 1,093,878 - RR42_RS04935 0.37 -0.6 1,093,878 - RR42_RS04935 0.37 -2.3 1,093,884 - RR42_RS04935 0.38 +1.6 1,093,945 - RR42_RS04935 0.45 -0.1
Or see this region's nucleotide sequence