Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04395 and RR42_RS04400 are separated by 30 nucleotides RR42_RS04400 and RR42_RS04405 are separated by 143 nucleotides RR42_RS04405 and RR42_RS04410 are separated by 20 nucleotides
RR42_RS04395: RR42_RS04395 - cysteine ABC transporter substrate-binding protein, at 978,133 to 978,930
_RS04395
RR42_RS04400: RR42_RS04400 - ABC transporter permease, at 978,961 to 979,620
_RS04400
RR42_RS04405: RR42_RS04405 - cyanophycin synthetase, at 979,764 to 982,334
_RS04405
RR42_RS04410: RR42_RS04410 - cyanophycin synthetase, at 982,355 to 984,991
_RS04410
Position (kb) 979
980
981
982
983 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 979.130 kb on - strand, within RR42_RS04400 at 979.180 kb on + strand, within RR42_RS04400 at 979.180 kb on + strand, within RR42_RS04400 at 979.181 kb on - strand, within RR42_RS04400 at 979.181 kb on - strand, within RR42_RS04400 at 979.184 kb on - strand, within RR42_RS04400 at 979.261 kb on + strand, within RR42_RS04400 at 979.262 kb on - strand, within RR42_RS04400 at 979.325 kb on - strand, within RR42_RS04400 at 979.325 kb on - strand, within RR42_RS04400 at 979.360 kb on + strand, within RR42_RS04400 at 979.361 kb on - strand, within RR42_RS04400 at 979.504 kb on + strand, within RR42_RS04400 at 979.522 kb on + strand, within RR42_RS04400 at 979.523 kb on - strand, within RR42_RS04400 at 979.539 kb on + strand, within RR42_RS04400 at 979.540 kb on - strand, within RR42_RS04400 at 979.540 kb on - strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.572 kb on + strand at 979.632 kb on + strand at 979.632 kb on + strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.644 kb on + strand at 979.644 kb on + strand at 979.790 kb on + strand at 979.866 kb on - strand at 979.991 kb on - strand at 979.991 kb on - strand at 980.180 kb on - strand, within RR42_RS04405 at 980.188 kb on - strand, within RR42_RS04405 at 980.188 kb on - strand, within RR42_RS04405 at 980.442 kb on - strand, within RR42_RS04405 at 980.812 kb on + strand, within RR42_RS04405 at 980.812 kb on + strand, within RR42_RS04405 at 981.483 kb on - strand, within RR42_RS04405 at 981.485 kb on - strand, within RR42_RS04405 at 981.530 kb on - strand, within RR42_RS04405 at 981.838 kb on + strand, within RR42_RS04405 at 982.024 kb on + strand, within RR42_RS04405 at 982.024 kb on + strand, within RR42_RS04405 at 982.032 kb on + strand, within RR42_RS04405 at 982.032 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.042 kb on + strand, within RR42_RS04405 at 982.043 kb on - strand, within RR42_RS04405 at 982.457 kb on + strand at 982.457 kb on + strand at 982.458 kb on - strand at 982.458 kb on - strand at 982.458 kb on - strand at 982.533 kb on + strand at 982.534 kb on - strand at 982.534 kb on - strand at 982.945 kb on - strand, within RR42_RS04410 at 983.025 kb on + strand, within RR42_RS04410 at 983.026 kb on - strand, within RR42_RS04410 at 983.026 kb on - strand, within RR42_RS04410 at 983.205 kb on + strand, within RR42_RS04410 at 983.205 kb on + strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.206 kb on - strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.229 kb on + strand, within RR42_RS04410 at 983.230 kb on - strand, within RR42_RS04410 at 983.230 kb on - strand, within RR42_RS04410 at 983.232 kb on + strand, within RR42_RS04410 at 983.292 kb on + strand, within RR42_RS04410
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 979,130 - RR42_RS04400 0.26 +1.2 979,180 + RR42_RS04400 0.33 -1.2 979,180 + RR42_RS04400 0.33 -0.8 979,181 - RR42_RS04400 0.33 -0.1 979,181 - RR42_RS04400 0.33 -0.6 979,184 - RR42_RS04400 0.34 +1.0 979,261 + RR42_RS04400 0.45 +0.0 979,262 - RR42_RS04400 0.46 -0.2 979,325 - RR42_RS04400 0.55 +1.3 979,325 - RR42_RS04400 0.55 -0.3 979,360 + RR42_RS04400 0.60 +0.4 979,361 - RR42_RS04400 0.61 -0.4 979,504 + RR42_RS04400 0.82 +0.3 979,522 + RR42_RS04400 0.85 -0.4 979,523 - RR42_RS04400 0.85 -0.8 979,539 + RR42_RS04400 0.88 -0.2 979,540 - RR42_RS04400 0.88 -0.7 979,540 - RR42_RS04400 0.88 -0.5 979,543 + RR42_RS04400 0.88 +0.9 979,543 + RR42_RS04400 0.88 +0.2 979,543 + RR42_RS04400 0.88 +1.7 979,572 + +0.1 979,632 + -0.6 979,632 + -2.6 979,633 - +0.3 979,633 - +0.3 979,633 - +0.2 979,633 - -2.1 979,633 - +0.9 979,644 + +0.4 979,644 + -0.3 979,790 + -2.3 979,866 - -0.4 979,991 - -2.4 979,991 - -1.0 980,180 - RR42_RS04405 0.16 -3.2 980,188 - RR42_RS04405 0.16 -2.6 980,188 - RR42_RS04405 0.16 -1.0 980,442 - RR42_RS04405 0.26 -3.2 980,812 + RR42_RS04405 0.41 -1.6 980,812 + RR42_RS04405 0.41 -2.5 981,483 - RR42_RS04405 0.67 -2.9 981,485 - RR42_RS04405 0.67 -2.3 981,530 - RR42_RS04405 0.69 -2.8 981,838 + RR42_RS04405 0.81 -2.7 982,024 + RR42_RS04405 0.88 -1.7 982,024 + RR42_RS04405 0.88 -2.2 982,032 + RR42_RS04405 0.88 -0.7 982,032 + RR42_RS04405 0.88 -3.4 982,042 + RR42_RS04405 0.89 -1.0 982,042 + RR42_RS04405 0.89 -2.1 982,042 + RR42_RS04405 0.89 +0.5 982,043 - RR42_RS04405 0.89 -2.0 982,457 + -1.9 982,457 + -1.2 982,458 - -2.5 982,458 - -1.0 982,458 - -0.6 982,533 + -2.0 982,534 - -3.1 982,534 - -0.8 982,945 - RR42_RS04410 0.22 -1.8 983,025 + RR42_RS04410 0.25 -1.2 983,026 - RR42_RS04410 0.25 -1.0 983,026 - RR42_RS04410 0.25 -3.4 983,205 + RR42_RS04410 0.32 -4.2 983,205 + RR42_RS04410 0.32 -2.4 983,206 - RR42_RS04410 0.32 -2.4 983,206 - RR42_RS04410 0.32 -2.2 983,206 - RR42_RS04410 0.32 -0.4 983,229 + RR42_RS04410 0.33 -2.0 983,229 + RR42_RS04410 0.33 -3.1 983,229 + RR42_RS04410 0.33 -3.3 983,229 + RR42_RS04410 0.33 +0.4 983,229 + RR42_RS04410 0.33 -1.6 983,229 + RR42_RS04410 0.33 -3.3 983,229 + RR42_RS04410 0.33 -1.7 983,230 - RR42_RS04410 0.33 -1.9 983,230 - RR42_RS04410 0.33 -3.1 983,232 + RR42_RS04410 0.33 +0.2 983,292 + RR42_RS04410 0.36 -2.6
Or see this region's nucleotide sequence