Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04070 and RR42_RS04075 are separated by 526 nucleotides RR42_RS04075 and RR42_RS04080 are separated by 40 nucleotides RR42_RS04080 and RR42_RS04085 are separated by 138 nucleotides
RR42_RS04070: RR42_RS04070 - quinone oxidoreductase, at 900,563 to 901,567
_RS04070
RR42_RS04075: RR42_RS04075 - molecular chaperone GroES, at 902,094 to 902,384
_RS04075
RR42_RS04080: RR42_RS04080 - molecular chaperone GroEL, at 902,425 to 904,068
_RS04080
RR42_RS04085: RR42_RS04085 - metallophosphoesterase, at 904,207 to 904,995
_RS04085
Position (kb)
902
903
904
905 Strain fitness (log2 ratio)
-2
-1
0
1 at 901.470 kb on - strand at 901.579 kb on + strand at 901.581 kb on + strand at 901.582 kb on - strand at 901.582 kb on - strand at 901.597 kb on + strand at 901.597 kb on + strand at 901.598 kb on - strand at 901.598 kb on - strand at 901.598 kb on - strand at 901.643 kb on + strand at 901.659 kb on + strand at 901.872 kb on + strand at 901.872 kb on + strand at 901.932 kb on + strand at 901.932 kb on + strand at 901.970 kb on + strand at 901.970 kb on + strand at 904.067 kb on - strand at 904.067 kb on - strand at 904.100 kb on + strand at 904.116 kb on - strand at 904.116 kb on - strand at 904.161 kb on + strand at 904.175 kb on - strand at 904.208 kb on + strand at 904.208 kb on + strand at 904.209 kb on - strand at 904.223 kb on + strand at 904.274 kb on + strand at 904.274 kb on + strand at 904.274 kb on + strand at 904.329 kb on + strand, within RR42_RS04085 at 904.335 kb on + strand, within RR42_RS04085 at 904.336 kb on - strand, within RR42_RS04085 at 904.665 kb on - strand, within RR42_RS04085 at 904.815 kb on - strand, within RR42_RS04085 at 904.815 kb on - strand, within RR42_RS04085 at 904.839 kb on - strand, within RR42_RS04085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 901,470 - -0.7 901,579 + -0.4 901,581 + -0.7 901,582 - +0.6 901,582 - -0.8 901,597 + -1.4 901,597 + +0.2 901,598 - -1.4 901,598 - -0.5 901,598 - +0.7 901,643 + -2.4 901,659 + +0.0 901,872 + +1.2 901,872 + +0.3 901,932 + -0.8 901,932 + -0.3 901,970 + -1.1 901,970 + -0.3 904,067 - -0.2 904,067 - -0.2 904,100 + +0.7 904,116 - -0.1 904,116 - -0.1 904,161 + +0.1 904,175 - -1.6 904,208 + +0.1 904,208 + -0.9 904,209 - +0.5 904,223 + +0.3 904,274 + -1.5 904,274 + +0.5 904,274 + +0.1 904,329 + RR42_RS04085 0.15 +0.2 904,335 + RR42_RS04085 0.16 -0.1 904,336 - RR42_RS04085 0.16 +0.8 904,665 - RR42_RS04085 0.58 +0.4 904,815 - RR42_RS04085 0.77 -0.1 904,815 - RR42_RS04085 0.77 -0.1 904,839 - RR42_RS04085 0.80 +1.0
Or see this region's nucleotide sequence