Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03080 and RR42_RS03085 are separated by 179 nucleotides RR42_RS03085 and RR42_RS03090 are separated by 327 nucleotides
RR42_RS03080: RR42_RS03080 - recombinase RecA, at 681,382 to 682,455
_RS03080
RR42_RS03085: RR42_RS03085 - RecX family transcriptional regulator, at 682,635 to 683,102
_RS03085
RR42_RS03090: RR42_RS03090 - hypothetical protein, at 683,430 to 684,104
_RS03090
Position (kb) 682
683
684 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 681.719 kb on - strand, within RR42_RS03080 at 682.164 kb on - strand, within RR42_RS03080 at 682.289 kb on - strand, within RR42_RS03080 at 682.333 kb on + strand, within RR42_RS03080 at 682.555 kb on - strand at 682.564 kb on + strand at 682.564 kb on + strand at 682.567 kb on - strand at 682.567 kb on - strand at 682.568 kb on + strand at 682.569 kb on - strand at 682.569 kb on - strand at 682.569 kb on - strand at 682.569 kb on - strand at 682.577 kb on + strand at 682.577 kb on + strand at 682.580 kb on - strand at 682.591 kb on - strand at 682.591 kb on - strand at 682.592 kb on + strand at 682.592 kb on + strand at 682.592 kb on + strand at 682.593 kb on - strand at 682.593 kb on - strand at 682.593 kb on - strand at 682.594 kb on + strand at 682.594 kb on + strand at 682.595 kb on - strand at 682.595 kb on - strand at 682.595 kb on - strand at 682.680 kb on + strand at 682.680 kb on + strand at 682.681 kb on - strand at 682.845 kb on + strand, within RR42_RS03085 at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.163 kb on - strand at 683.163 kb on - strand at 683.194 kb on + strand at 683.215 kb on + strand at 683.215 kb on + strand at 683.374 kb on - strand at 683.429 kb on + strand at 683.429 kb on + strand at 683.430 kb on - strand at 683.493 kb on + strand at 683.493 kb on + strand at 683.494 kb on - strand at 683.498 kb on + strand, within RR42_RS03090 at 683.498 kb on + strand, within RR42_RS03090 at 683.499 kb on - strand, within RR42_RS03090 at 683.512 kb on + strand, within RR42_RS03090 at 683.578 kb on + strand, within RR42_RS03090 at 683.818 kb on + strand, within RR42_RS03090 at 683.894 kb on + strand, within RR42_RS03090 at 683.894 kb on + strand, within RR42_RS03090 at 683.907 kb on + strand, within RR42_RS03090 at 683.907 kb on + strand, within RR42_RS03090 at 683.907 kb on + strand, within RR42_RS03090 at 683.953 kb on + strand, within RR42_RS03090 at 683.953 kb on + strand, within RR42_RS03090 at 683.976 kb on + strand, within RR42_RS03090
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 681,719 - RR42_RS03080 0.31 -0.8 682,164 - RR42_RS03080 0.73 +0.6 682,289 - RR42_RS03080 0.84 -1.2 682,333 + RR42_RS03080 0.89 -0.4 682,555 - -0.2 682,564 + -0.1 682,564 + +1.2 682,567 - -1.2 682,567 - +0.1 682,568 + -0.0 682,569 - +0.8 682,569 - -2.0 682,569 - +0.6 682,569 - +1.4 682,577 + -0.4 682,577 + -0.0 682,580 - +1.8 682,591 - +0.1 682,591 - +2.6 682,592 + -1.2 682,592 + +0.2 682,592 + +0.2 682,593 - +0.6 682,593 - +0.2 682,593 - +0.2 682,594 + +1.2 682,594 + -0.2 682,595 - +0.2 682,595 - +0.3 682,595 - +0.7 682,680 + +0.6 682,680 + -0.1 682,681 - -1.1 682,845 + RR42_RS03085 0.45 -0.9 683,162 + +0.2 683,162 + -2.2 683,162 + -0.9 683,162 + -1.3 683,162 + -0.2 683,162 + +0.1 683,163 - +0.4 683,163 - -1.0 683,194 + +0.5 683,215 + +0.7 683,215 + -0.8 683,374 - +0.9 683,429 + +1.1 683,429 + +0.1 683,430 - +1.2 683,493 + -2.2 683,493 + +0.2 683,494 - -1.3 683,498 + RR42_RS03090 0.10 +0.9 683,498 + RR42_RS03090 0.10 -1.5 683,499 - RR42_RS03090 0.10 -1.3 683,512 + RR42_RS03090 0.12 +0.8 683,578 + RR42_RS03090 0.22 +0.5 683,818 + RR42_RS03090 0.57 -0.0 683,894 + RR42_RS03090 0.69 -1.1 683,894 + RR42_RS03090 0.69 -1.3 683,907 + RR42_RS03090 0.71 +0.2 683,907 + RR42_RS03090 0.71 +0.1 683,907 + RR42_RS03090 0.71 -2.8 683,953 + RR42_RS03090 0.77 -0.3 683,953 + RR42_RS03090 0.77 +0.9 683,976 + RR42_RS03090 0.81 +0.2
Or see this region's nucleotide sequence