Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03030 and RR42_RS03035 overlap by 4 nucleotides RR42_RS03035 and RR42_RS03040 overlap by 4 nucleotides RR42_RS03040 and RR42_RS03045 are separated by 110 nucleotides
RR42_RS03030: RR42_RS03030 - required for 4-hydroxybenzoate transport, together with NodT, MFP, and FUSC proteins (RR42_RS03040, RR42_RS03035, and RR42_RS03025) <small>(from data)</small>, at 671,455 to 671,664
_RS03030
RR42_RS03035: RR42_RS03035 - membrane protein, at 671,661 to 672,629
_RS03035
RR42_RS03040: RR42_RS03040 - Outer membrane protein (NodT-like) required for 4-hydroxybenzoate transport, together with FUSC, MFP, and DUF1656 proteins (RR42_RS03025, RR42_RS03035, and RR42_RS03030) <small>(from data)</small>, at 672,626 to 674,089
_RS03040
RR42_RS03045: RR42_RS03045 - dioxygenase, at 674,200 to 675,021
_RS03045
Position (kb) 672
673
674
675 Strain fitness (log2 ratio) -2
-1
0
1
2 at 671.639 kb on - strand, within RR42_RS03030 at 671.639 kb on - strand, within RR42_RS03030 at 671.678 kb on + strand at 671.699 kb on + strand at 671.752 kb on - strand at 671.754 kb on + strand at 671.754 kb on + strand at 672.147 kb on + strand, within RR42_RS03035 at 672.704 kb on + strand at 672.704 kb on + strand at 672.818 kb on + strand, within RR42_RS03040 at 673.181 kb on + strand, within RR42_RS03040 at 673.182 kb on - strand, within RR42_RS03040 at 673.182 kb on - strand, within RR42_RS03040 at 673.182 kb on - strand, within RR42_RS03040 at 673.333 kb on - strand, within RR42_RS03040 at 673.436 kb on + strand, within RR42_RS03040 at 673.814 kb on + strand, within RR42_RS03040 at 673.815 kb on - strand, within RR42_RS03040 at 673.897 kb on - strand, within RR42_RS03040 at 673.897 kb on - strand, within RR42_RS03040 at 673.897 kb on - strand, within RR42_RS03040 at 673.898 kb on + strand, within RR42_RS03040 at 673.898 kb on + strand, within RR42_RS03040 at 673.898 kb on + strand, within RR42_RS03040 at 673.899 kb on - strand, within RR42_RS03040 at 673.919 kb on + strand, within RR42_RS03040 at 673.920 kb on - strand, within RR42_RS03040 at 674.127 kb on - strand at 674.216 kb on - strand at 674.407 kb on + strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.473 kb on + strand, within RR42_RS03045 at 674.474 kb on - strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.533 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.819 kb on - strand, within RR42_RS03045 at 674.969 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 671,639 - RR42_RS03030 0.88 -2.6 671,639 - RR42_RS03030 0.88 +0.9 671,678 + -1.9 671,699 + +0.7 671,752 - +0.2 671,754 + +0.9 671,754 + +0.2 672,147 + RR42_RS03035 0.50 +0.2 672,704 + +1.2 672,704 + +1.2 672,818 + RR42_RS03040 0.13 +0.0 673,181 + RR42_RS03040 0.38 +0.3 673,182 - RR42_RS03040 0.38 +1.2 673,182 - RR42_RS03040 0.38 +1.0 673,182 - RR42_RS03040 0.38 -0.8 673,333 - RR42_RS03040 0.48 +0.6 673,436 + RR42_RS03040 0.55 -0.2 673,814 + RR42_RS03040 0.81 +0.1 673,815 - RR42_RS03040 0.81 +0.8 673,897 - RR42_RS03040 0.87 +0.2 673,897 - RR42_RS03040 0.87 +1.0 673,897 - RR42_RS03040 0.87 -1.3 673,898 + RR42_RS03040 0.87 +0.1 673,898 + RR42_RS03040 0.87 +0.8 673,898 + RR42_RS03040 0.87 +0.7 673,899 - RR42_RS03040 0.87 -0.3 673,919 + RR42_RS03040 0.88 +0.2 673,920 - RR42_RS03040 0.88 -0.3 674,127 - +1.4 674,216 - -0.5 674,407 + RR42_RS03045 0.25 -0.3 674,408 - RR42_RS03045 0.25 -1.5 674,408 - RR42_RS03045 0.25 +0.4 674,408 - RR42_RS03045 0.25 -0.9 674,473 + RR42_RS03045 0.33 +0.7 674,474 - RR42_RS03045 0.33 +0.4 674,530 + RR42_RS03045 0.40 -0.1 674,530 + RR42_RS03045 0.40 +2.2 674,530 + RR42_RS03045 0.40 -0.5 674,530 + RR42_RS03045 0.40 -0.2 674,530 + RR42_RS03045 0.40 -1.5 674,531 - RR42_RS03045 0.40 +0.2 674,531 - RR42_RS03045 0.40 +0.2 674,531 - RR42_RS03045 0.40 -0.3 674,531 - RR42_RS03045 0.40 +0.4 674,531 - RR42_RS03045 0.40 +0.4 674,531 - RR42_RS03045 0.40 +0.0 674,531 - RR42_RS03045 0.40 -0.3 674,531 - RR42_RS03045 0.40 +0.2 674,532 + RR42_RS03045 0.40 -0.1 674,532 + RR42_RS03045 0.40 -0.6 674,532 + RR42_RS03045 0.40 -0.5 674,532 + RR42_RS03045 0.40 -0.2 674,532 + RR42_RS03045 0.40 +0.7 674,533 - RR42_RS03045 0.41 -1.2 674,552 - RR42_RS03045 0.43 -2.0 674,552 - RR42_RS03045 0.43 +0.6 674,819 - RR42_RS03045 0.75 -0.7 674,969 + +0.6
Or see this region's nucleotide sequence