Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03015 and RR42_RS03020 are separated by 354 nucleotides RR42_RS03020 and RR42_RS03025 are separated by 4 nucleotides
RR42_RS03015: RR42_RS03015 - enoyl-CoA hydratase, at 667,753 to 668,550
_RS03015
RR42_RS03020: RR42_RS03020 - MarR family transcriptional regulator, at 668,905 to 669,366
_RS03020
RR42_RS03025: RR42_RS03025 - Inner membrane protein (FUSC-like) required for 4-hydroxybenzoate transport, together with NodT, MFP, and DUF1656 proteins (RR42_RS03040, RR42_RS03035, and RR42_RS03030) <small>(from data)</small>, at 669,371 to 671,365
_RS03025
Position (kb) 668
669
670 Strain fitness (log2 ratio) -1
0
1 at 667.969 kb on + strand, within RR42_RS03015 at 667.969 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.973 kb on - strand, within RR42_RS03015 at 668.198 kb on - strand, within RR42_RS03015 at 668.729 kb on - strand at 668.730 kb on + strand at 668.731 kb on - strand at 668.838 kb on - strand at 669.085 kb on + strand, within RR42_RS03020 at 669.086 kb on - strand, within RR42_RS03020 at 669.364 kb on + strand at 669.364 kb on + strand at 669.455 kb on + strand at 669.497 kb on + strand at 669.497 kb on + strand at 669.498 kb on - strand at 669.498 kb on - strand at 669.498 kb on - strand at 669.500 kb on - strand at 669.500 kb on - strand at 669.500 kb on - strand at 669.695 kb on + strand, within RR42_RS03025 at 669.695 kb on + strand, within RR42_RS03025 at 669.695 kb on + strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.716 kb on + strand, within RR42_RS03025 at 669.717 kb on - strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.719 kb on - strand, within RR42_RS03025 at 669.739 kb on - strand, within RR42_RS03025 at 669.918 kb on - strand, within RR42_RS03025 at 670.023 kb on - strand, within RR42_RS03025 at 670.111 kb on + strand, within RR42_RS03025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 667,969 + RR42_RS03015 0.27 +0.0 667,969 + RR42_RS03015 0.27 +0.2 667,972 + RR42_RS03015 0.27 +0.8 667,972 + RR42_RS03015 0.27 -0.2 667,972 + RR42_RS03015 0.27 -1.0 667,973 - RR42_RS03015 0.28 +0.6 668,198 - RR42_RS03015 0.56 -0.7 668,729 - +0.7 668,730 + -1.6 668,731 - -0.3 668,838 - +0.3 669,085 + RR42_RS03020 0.39 -0.8 669,086 - RR42_RS03020 0.39 -0.8 669,364 + +0.0 669,364 + +0.1 669,455 + +0.8 669,497 + +0.9 669,497 + -0.2 669,498 - +0.7 669,498 - +0.3 669,498 - -0.2 669,500 - +0.0 669,500 - +0.5 669,500 - +0.2 669,695 + RR42_RS03025 0.16 -0.7 669,695 + RR42_RS03025 0.16 -0.2 669,695 + RR42_RS03025 0.16 -0.1 669,696 - RR42_RS03025 0.16 -0.2 669,696 - RR42_RS03025 0.16 -0.2 669,696 - RR42_RS03025 0.16 +0.4 669,696 - RR42_RS03025 0.16 -0.2 669,696 - RR42_RS03025 0.16 +0.6 669,716 + RR42_RS03025 0.17 -0.8 669,717 - RR42_RS03025 0.17 -0.6 669,718 + RR42_RS03025 0.17 +0.5 669,718 + RR42_RS03025 0.17 +0.4 669,718 + RR42_RS03025 0.17 -0.5 669,718 + RR42_RS03025 0.17 +1.0 669,719 - RR42_RS03025 0.17 -0.8 669,739 - RR42_RS03025 0.18 -1.3 669,918 - RR42_RS03025 0.27 -0.1 670,023 - RR42_RS03025 0.33 -0.7 670,111 + RR42_RS03025 0.37 -0.5
Or see this region's nucleotide sequence