Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03000 and RR42_RS03005 are separated by 162 nucleotides RR42_RS03005 and RR42_RS03010 are separated by 44 nucleotides RR42_RS03010 and RR42_RS03015 are separated by 45 nucleotides
RR42_RS03000: RR42_RS03000 - ABC transporter substrate-binding protein, at 664,589 to 665,557
_RS03000
RR42_RS03005: RR42_RS03005 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 665,720 to 666,484
_RS03005
RR42_RS03010: RR42_RS03010 - acetyl-CoA acetyltransferase, at 666,529 to 667,707
_RS03010
RR42_RS03015: RR42_RS03015 - enoyl-CoA hydratase, at 667,753 to 668,550
_RS03015
Position (kb) 666
667
668 Strain fitness (log2 ratio) -2
-1
0
1
2 at 665.539 kb on + strand at 665.540 kb on - strand at 665.791 kb on + strand at 665.792 kb on - strand at 665.902 kb on + strand, within RR42_RS03005 at 665.903 kb on - strand, within RR42_RS03005 at 666.006 kb on + strand, within RR42_RS03005 at 666.033 kb on + strand, within RR42_RS03005 at 666.033 kb on + strand, within RR42_RS03005 at 666.489 kb on + strand at 666.770 kb on + strand, within RR42_RS03010 at 667.379 kb on + strand, within RR42_RS03010 at 667.379 kb on + strand, within RR42_RS03010 at 667.867 kb on - strand, within RR42_RS03015 at 667.969 kb on + strand, within RR42_RS03015 at 667.969 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.973 kb on - strand, within RR42_RS03015 at 668.198 kb on - strand, within RR42_RS03015
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 665,539 + -0.3 665,540 - -0.4 665,791 + +0.2 665,792 - +0.5 665,902 + RR42_RS03005 0.24 -1.8 665,903 - RR42_RS03005 0.24 -0.5 666,006 + RR42_RS03005 0.37 +2.6 666,033 + RR42_RS03005 0.41 -0.0 666,033 + RR42_RS03005 0.41 +0.1 666,489 + -0.8 666,770 + RR42_RS03010 0.20 -0.2 667,379 + RR42_RS03010 0.72 +0.0 667,379 + RR42_RS03010 0.72 +1.9 667,867 - RR42_RS03015 0.14 +0.2 667,969 + RR42_RS03015 0.27 +0.0 667,969 + RR42_RS03015 0.27 +0.2 667,972 + RR42_RS03015 0.27 +0.8 667,972 + RR42_RS03015 0.27 -0.2 667,972 + RR42_RS03015 0.27 -1.0 667,973 - RR42_RS03015 0.28 +0.6 668,198 - RR42_RS03015 0.56 -0.7
Or see this region's nucleotide sequence