Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02850 and RR42_RS02855 are separated by 40 nucleotides RR42_RS02855 and RR42_RS02860 are separated by 94 nucleotides RR42_RS02860 and RR42_RS02865 are separated by 123 nucleotides
RR42_RS02850: RR42_RS02850 - phoH-like protein, at 635,155 to 636,150
_RS02850
RR42_RS02855: RR42_RS02855 - dimethylallyladenosine tRNA methylthiotransferase, at 636,191 to 637,543
_RS02855
RR42_RS02860: RR42_RS02860 - hypothetical protein, at 637,638 to 638,018
_RS02860
RR42_RS02865: RR42_RS02865 - chloride channel protein, at 638,142 to 639,830
_RS02865
Position (kb) 636
637
638 Strain fitness (log2 ratio) -1
0
1
2 at 635.228 kb on - strand at 635.466 kb on - strand, within RR42_RS02850 at 635.466 kb on - strand, within RR42_RS02850 at 635.550 kb on - strand, within RR42_RS02850 at 635.564 kb on + strand, within RR42_RS02850 at 635.565 kb on - strand, within RR42_RS02850 at 635.565 kb on - strand, within RR42_RS02850 at 635.699 kb on + strand, within RR42_RS02850 at 635.700 kb on - strand, within RR42_RS02850 at 635.874 kb on - strand, within RR42_RS02850 at 635.958 kb on - strand, within RR42_RS02850 at 636.272 kb on - strand at 636.272 kb on - strand at 636.536 kb on - strand, within RR42_RS02855 at 636.688 kb on - strand, within RR42_RS02855 at 636.789 kb on + strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.838 kb on - strand, within RR42_RS02855 at 636.931 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855 at 637.475 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.519 kb on - strand at 637.634 kb on - strand at 637.634 kb on - strand at 637.639 kb on + strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.640 kb on - strand at 637.763 kb on + strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 637.960 kb on - strand, within RR42_RS02860 at 638.106 kb on + strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.107 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.110 kb on - strand at 638.143 kb on + strand at 638.144 kb on - strand at 638.144 kb on - strand at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.413 kb on + strand, within RR42_RS02865 at 638.414 kb on - strand, within RR42_RS02865 at 638.419 kb on + strand, within RR42_RS02865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 635,228 - +0.3 635,466 - RR42_RS02850 0.31 +1.0 635,466 - RR42_RS02850 0.31 -0.5 635,550 - RR42_RS02850 0.40 -0.3 635,564 + RR42_RS02850 0.41 +1.3 635,565 - RR42_RS02850 0.41 -1.5 635,565 - RR42_RS02850 0.41 +1.3 635,699 + RR42_RS02850 0.55 -0.8 635,700 - RR42_RS02850 0.55 -0.4 635,874 - RR42_RS02850 0.72 +0.5 635,958 - RR42_RS02850 0.81 +0.5 636,272 - +0.2 636,272 - -0.8 636,536 - RR42_RS02855 0.25 -0.3 636,688 - RR42_RS02855 0.37 -0.0 636,789 + RR42_RS02855 0.44 -0.3 636,790 - RR42_RS02855 0.44 +0.8 636,790 - RR42_RS02855 0.44 +0.3 636,790 - RR42_RS02855 0.44 -1.5 636,838 - RR42_RS02855 0.48 -0.2 636,931 - RR42_RS02855 0.55 -0.8 637,005 - RR42_RS02855 0.60 -0.7 637,005 - RR42_RS02855 0.60 -0.0 637,060 - RR42_RS02855 0.64 +0.2 637,060 - RR42_RS02855 0.64 -0.0 637,069 - RR42_RS02855 0.65 -1.3 637,069 - RR42_RS02855 0.65 -0.9 637,475 - -0.4 637,519 - -1.5 637,519 - +0.7 637,519 - +0.2 637,634 - -1.5 637,634 - -1.5 637,639 + -1.5 637,640 - +1.0 637,640 - +1.5 637,640 - +0.1 637,640 - +0.3 637,763 + RR42_RS02860 0.33 -0.3 637,960 - RR42_RS02860 0.85 -1.3 637,960 - RR42_RS02860 0.85 +0.7 638,106 + -0.2 638,107 - +1.3 638,107 - +0.6 638,107 - -0.7 638,107 - -1.5 638,110 - -0.6 638,110 - +0.3 638,110 - -0.1 638,110 - +0.1 638,110 - +0.1 638,143 + -1.0 638,144 - +1.9 638,144 - -0.1 638,413 + RR42_RS02865 0.16 +1.7 638,413 + RR42_RS02865 0.16 -1.1 638,413 + RR42_RS02865 0.16 -0.2 638,413 + RR42_RS02865 0.16 +0.3 638,414 - RR42_RS02865 0.16 +0.8 638,419 + RR42_RS02865 0.16 -1.0
Or see this region's nucleotide sequence