Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02780 and RR42_RS02785 are separated by 20 nucleotides RR42_RS02785 and RR42_RS02790 are separated by 158 nucleotides
RR42_RS02780: RR42_RS02780 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 619,423 to 620,472
_RS02780
RR42_RS02785: RR42_RS02785 - dimethyladenosine transferase, at 620,493 to 621,314
_RS02785
RR42_RS02790: RR42_RS02790 - tryptophanyl-tRNA synthetase, at 621,473 to 622,495
_RS02790
Position (kb) 620
621
622 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 620.459 kb on + strand at 620.488 kb on + strand at 620.488 kb on + strand at 620.489 kb on - strand at 620.489 kb on - strand at 620.492 kb on + strand at 620.492 kb on + strand at 620.506 kb on + strand at 620.985 kb on + strand, within RR42_RS02785 at 620.985 kb on + strand, within RR42_RS02785 at 621.185 kb on + strand, within RR42_RS02785 at 621.185 kb on + strand, within RR42_RS02785 at 621.186 kb on - strand, within RR42_RS02785 at 621.274 kb on - strand at 621.308 kb on + strand at 621.309 kb on - strand at 621.458 kb on - strand at 621.554 kb on + strand at 621.555 kb on - strand at 621.555 kb on - strand at 621.605 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 622.160 kb on + strand, within RR42_RS02790 at 622.160 kb on + strand, within RR42_RS02790 at 622.222 kb on + strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.227 kb on - strand, within RR42_RS02790
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 620,459 + -0.6 620,488 + -0.4 620,488 + -0.6 620,489 - +0.7 620,489 - -1.1 620,492 + -2.4 620,492 + -0.6 620,506 + -1.4 620,985 + RR42_RS02785 0.60 -3.4 620,985 + RR42_RS02785 0.60 -1.8 621,185 + RR42_RS02785 0.84 -0.3 621,185 + RR42_RS02785 0.84 -0.7 621,186 - RR42_RS02785 0.84 +1.0 621,274 - +0.6 621,308 + +0.2 621,309 - -1.1 621,458 - -2.5 621,554 + -1.3 621,555 - -0.6 621,555 - -1.5 621,605 + RR42_RS02790 0.13 +0.3 621,779 + RR42_RS02790 0.30 +0.3 621,779 + RR42_RS02790 0.30 +2.9 621,779 + RR42_RS02790 0.30 +0.2 621,779 + RR42_RS02790 0.30 -0.7 622,160 + RR42_RS02790 0.67 -0.4 622,160 + RR42_RS02790 0.67 -0.8 622,222 + RR42_RS02790 0.73 -0.1 622,223 - RR42_RS02790 0.73 -1.1 622,223 - RR42_RS02790 0.73 +0.4 622,223 - RR42_RS02790 0.73 +1.3 622,227 - RR42_RS02790 0.74 -0.2
Or see this region's nucleotide sequence