Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02745 and RR42_RS02750 are separated by 81 nucleotides RR42_RS02750 and RR42_RS02755 are separated by 17 nucleotides RR42_RS02755 and RR42_RS02760 overlap by 4 nucleotides
RR42_RS02745: RR42_RS02745 - Xaa-Pro aminopeptidase, at 610,964 to 612,346
_RS02745
RR42_RS02750: RR42_RS02750 - mannose-1-phosphate guanylyltransferase, at 612,428 to 613,168
_RS02750
RR42_RS02755: RR42_RS02755 - branched-chain amino acid transporter, at 613,186 to 613,524
_RS02755
RR42_RS02760: RR42_RS02760 - branched-chain amino acid ABC transporter permease, at 613,521 to 614,273
_RS02760
Position (kb) 612
613
614 Strain fitness (log2 ratio) -2
-1
0
1 at 611.461 kb on - strand, within RR42_RS02745 at 611.461 kb on - strand, within RR42_RS02745 at 611.461 kb on - strand, within RR42_RS02745 at 611.461 kb on - strand, within RR42_RS02745 at 611.644 kb on - strand, within RR42_RS02745 at 611.644 kb on - strand, within RR42_RS02745 at 611.644 kb on - strand, within RR42_RS02745 at 611.644 kb on - strand, within RR42_RS02745 at 611.704 kb on - strand, within RR42_RS02745 at 612.052 kb on - strand, within RR42_RS02745 at 612.181 kb on - strand, within RR42_RS02745 at 612.187 kb on - strand, within RR42_RS02745 at 612.187 kb on - strand, within RR42_RS02745 at 612.187 kb on - strand, within RR42_RS02745 at 612.187 kb on - strand, within RR42_RS02745 at 612.187 kb on - strand, within RR42_RS02745 at 612.187 kb on - strand, within RR42_RS02745 at 612.382 kb on + strand at 612.570 kb on + strand, within RR42_RS02750 at 612.571 kb on - strand, within RR42_RS02750 at 612.573 kb on + strand, within RR42_RS02750 at 612.630 kb on + strand, within RR42_RS02750 at 612.630 kb on + strand, within RR42_RS02750 at 612.630 kb on + strand, within RR42_RS02750 at 612.630 kb on + strand, within RR42_RS02750 at 612.631 kb on - strand, within RR42_RS02750 at 612.640 kb on + strand, within RR42_RS02750 at 612.819 kb on + strand, within RR42_RS02750 at 612.819 kb on + strand, within RR42_RS02750 at 612.819 kb on + strand, within RR42_RS02750 at 612.942 kb on + strand, within RR42_RS02750 at 612.942 kb on + strand, within RR42_RS02750 at 613.187 kb on + strand at 613.188 kb on - strand at 613.188 kb on - strand at 613.188 kb on - strand at 613.211 kb on + strand at 613.211 kb on + strand at 613.211 kb on + strand at 613.211 kb on + strand at 613.212 kb on - strand at 613.656 kb on + strand, within RR42_RS02760 at 613.656 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.927 kb on + strand, within RR42_RS02760 at 613.928 kb on - strand, within RR42_RS02760 at 614.161 kb on + strand, within RR42_RS02760 at 614.161 kb on + strand, within RR42_RS02760 at 614.161 kb on + strand, within RR42_RS02760 at 614.162 kb on - strand, within RR42_RS02760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 611,461 - RR42_RS02745 0.36 -0.1 611,461 - RR42_RS02745 0.36 +1.6 611,461 - RR42_RS02745 0.36 +1.0 611,461 - RR42_RS02745 0.36 +0.9 611,644 - RR42_RS02745 0.49 +1.3 611,644 - RR42_RS02745 0.49 +0.9 611,644 - RR42_RS02745 0.49 +0.6 611,644 - RR42_RS02745 0.49 +1.6 611,704 - RR42_RS02745 0.54 +1.3 612,052 - RR42_RS02745 0.79 +1.6 612,181 - RR42_RS02745 0.88 +1.5 612,187 - RR42_RS02745 0.88 +0.7 612,187 - RR42_RS02745 0.88 +1.0 612,187 - RR42_RS02745 0.88 +0.5 612,187 - RR42_RS02745 0.88 +1.3 612,187 - RR42_RS02745 0.88 +1.3 612,187 - RR42_RS02745 0.88 +0.1 612,382 + +0.1 612,570 + RR42_RS02750 0.19 -0.7 612,571 - RR42_RS02750 0.19 -2.0 612,573 + RR42_RS02750 0.20 -0.6 612,630 + RR42_RS02750 0.27 -1.4 612,630 + RR42_RS02750 0.27 -0.9 612,630 + RR42_RS02750 0.27 +1.0 612,630 + RR42_RS02750 0.27 -0.3 612,631 - RR42_RS02750 0.27 -1.2 612,640 + RR42_RS02750 0.29 -1.4 612,819 + RR42_RS02750 0.53 -0.5 612,819 + RR42_RS02750 0.53 -0.4 612,819 + RR42_RS02750 0.53 +0.1 612,942 + RR42_RS02750 0.69 -1.2 612,942 + RR42_RS02750 0.69 -0.4 613,187 + -0.6 613,188 - +0.1 613,188 - +1.1 613,188 - -0.1 613,211 + -1.1 613,211 + +0.3 613,211 + +0.1 613,211 + +0.6 613,212 - -0.1 613,656 + RR42_RS02760 0.18 -1.3 613,656 + RR42_RS02760 0.18 -0.1 613,927 + RR42_RS02760 0.54 +0.2 613,927 + RR42_RS02760 0.54 -0.4 613,927 + RR42_RS02760 0.54 -0.3 613,927 + RR42_RS02760 0.54 -2.0 613,928 - RR42_RS02760 0.54 -0.1 614,161 + RR42_RS02760 0.85 -0.8 614,161 + RR42_RS02760 0.85 -0.6 614,161 + RR42_RS02760 0.85 +0.3 614,162 - RR42_RS02760 0.85 -1.0
Or see this region's nucleotide sequence