Experiment: R2A with Sisomicin 0.035 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02570 and RR42_RS02575 are separated by 364 nucleotides RR42_RS02575 and RR42_RS02580 are separated by 63 nucleotides RR42_RS02580 and RR42_RS02585 are separated by 11 nucleotides
RR42_RS02570: RR42_RS02570 - glutamate dehydrogenase, at 577,482 to 578,789
_RS02570
RR42_RS02575: RR42_RS02575 - ABC transporter, at 579,154 to 580,053
_RS02575
RR42_RS02580: RR42_RS02580 - amino acid ABC transporter permease, at 580,117 to 580,845
_RS02580
RR42_RS02585: RR42_RS02585 - amino acid ABC transporter permease, at 580,857 to 581,540
_RS02585
Position (kb) 579
580
581 Strain fitness (log2 ratio) -2
-1
0
1
2 at 578.283 kb on + strand, within RR42_RS02570 at 578.283 kb on + strand, within RR42_RS02570 at 578.322 kb on + strand, within RR42_RS02570 at 578.322 kb on + strand, within RR42_RS02570 at 578.322 kb on + strand, within RR42_RS02570 at 578.323 kb on - strand, within RR42_RS02570 at 578.323 kb on - strand, within RR42_RS02570 at 578.323 kb on - strand, within RR42_RS02570 at 578.535 kb on - strand, within RR42_RS02570 at 578.586 kb on + strand, within RR42_RS02570 at 578.599 kb on + strand, within RR42_RS02570 at 578.600 kb on - strand, within RR42_RS02570 at 578.712 kb on - strand at 578.850 kb on + strand at 578.901 kb on + strand at 578.901 kb on + strand at 578.902 kb on - strand at 579.091 kb on - strand at 579.091 kb on - strand at 579.091 kb on - strand at 579.153 kb on + strand at 579.154 kb on - strand at 579.154 kb on - strand at 579.154 kb on - strand at 579.271 kb on - strand, within RR42_RS02575 at 579.271 kb on - strand, within RR42_RS02575 at 579.271 kb on - strand, within RR42_RS02575 at 579.307 kb on + strand, within RR42_RS02575 at 579.307 kb on + strand, within RR42_RS02575 at 579.308 kb on - strand, within RR42_RS02575 at 579.308 kb on - strand, within RR42_RS02575 at 579.343 kb on + strand, within RR42_RS02575 at 579.344 kb on - strand, within RR42_RS02575 at 579.344 kb on - strand, within RR42_RS02575 at 579.844 kb on + strand, within RR42_RS02575 at 579.845 kb on - strand, within RR42_RS02575 at 579.940 kb on + strand, within RR42_RS02575 at 579.940 kb on + strand, within RR42_RS02575 at 579.940 kb on + strand, within RR42_RS02575 at 580.054 kb on + strand at 580.055 kb on - strand at 580.123 kb on + strand at 580.123 kb on + strand at 580.123 kb on + strand at 580.124 kb on - strand at 580.124 kb on - strand at 580.124 kb on - strand at 580.142 kb on + strand at 580.143 kb on - strand at 580.143 kb on - strand at 580.143 kb on - strand at 580.174 kb on + strand at 580.174 kb on + strand at 580.175 kb on - strand at 580.316 kb on - strand, within RR42_RS02580 at 580.316 kb on - strand, within RR42_RS02580 at 580.316 kb on - strand, within RR42_RS02580 at 580.332 kb on + strand, within RR42_RS02580 at 580.333 kb on - strand, within RR42_RS02580 at 580.335 kb on + strand, within RR42_RS02580 at 580.570 kb on + strand, within RR42_RS02580 at 580.571 kb on - strand, within RR42_RS02580 at 580.576 kb on + strand, within RR42_RS02580 at 580.577 kb on - strand, within RR42_RS02580 at 580.577 kb on - strand, within RR42_RS02580 at 580.583 kb on + strand, within RR42_RS02580 at 580.583 kb on + strand, within RR42_RS02580 at 580.714 kb on + strand, within RR42_RS02580 at 580.714 kb on + strand, within RR42_RS02580 at 580.715 kb on - strand, within RR42_RS02580 at 580.729 kb on + strand, within RR42_RS02580 at 580.759 kb on + strand, within RR42_RS02580 at 580.760 kb on - strand, within RR42_RS02580 at 580.760 kb on - strand, within RR42_RS02580 at 580.760 kb on - strand, within RR42_RS02580 at 580.843 kb on + strand at 580.843 kb on + strand at 580.843 kb on + strand at 580.844 kb on - strand at 580.844 kb on - strand at 580.844 kb on - strand at 580.863 kb on + strand at 580.863 kb on + strand at 580.863 kb on + strand at 580.863 kb on + strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.864 kb on - strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.865 kb on + strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 580.866 kb on - strand at 581.037 kb on + strand, within RR42_RS02585 at 581.037 kb on + strand, within RR42_RS02585 at 581.037 kb on + strand, within RR42_RS02585 at 581.038 kb on - strand, within RR42_RS02585 at 581.038 kb on - strand, within RR42_RS02585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.035 mg/ml remove 578,283 + RR42_RS02570 0.61 -2.5 578,283 + RR42_RS02570 0.61 +0.3 578,322 + RR42_RS02570 0.64 -1.5 578,322 + RR42_RS02570 0.64 -0.3 578,322 + RR42_RS02570 0.64 -1.1 578,323 - RR42_RS02570 0.64 -1.3 578,323 - RR42_RS02570 0.64 +1.3 578,323 - RR42_RS02570 0.64 -0.3 578,535 - RR42_RS02570 0.81 -0.5 578,586 + RR42_RS02570 0.84 -0.6 578,599 + RR42_RS02570 0.85 -0.6 578,600 - RR42_RS02570 0.85 -1.3 578,712 - -0.9 578,850 + +0.2 578,901 + +0.3 578,901 + +0.4 578,902 - -1.7 579,091 - +0.7 579,091 - -1.1 579,091 - +0.8 579,153 + +1.3 579,154 - +0.3 579,154 - +0.8 579,154 - -0.2 579,271 - RR42_RS02575 0.13 +0.0 579,271 - RR42_RS02575 0.13 -0.3 579,271 - RR42_RS02575 0.13 -0.0 579,307 + RR42_RS02575 0.17 -0.5 579,307 + RR42_RS02575 0.17 -0.8 579,308 - RR42_RS02575 0.17 +0.8 579,308 - RR42_RS02575 0.17 +1.7 579,343 + RR42_RS02575 0.21 +0.1 579,344 - RR42_RS02575 0.21 -0.2 579,344 - RR42_RS02575 0.21 -0.1 579,844 + RR42_RS02575 0.77 -0.1 579,845 - RR42_RS02575 0.77 +2.0 579,940 + RR42_RS02575 0.87 +1.0 579,940 + RR42_RS02575 0.87 -2.6 579,940 + RR42_RS02575 0.87 +0.1 580,054 + +0.3 580,055 - -0.3 580,123 + -0.1 580,123 + +0.8 580,123 + -0.5 580,124 - -0.4 580,124 - -0.2 580,124 - +1.3 580,142 + -0.6 580,143 - +0.9 580,143 - +0.3 580,143 - +0.3 580,174 + -0.4 580,174 + +0.4 580,175 - -2.1 580,316 - RR42_RS02580 0.27 -0.5 580,316 - RR42_RS02580 0.27 -0.2 580,316 - RR42_RS02580 0.27 +0.3 580,332 + RR42_RS02580 0.29 -1.2 580,333 - RR42_RS02580 0.30 -1.3 580,335 + RR42_RS02580 0.30 +1.7 580,570 + RR42_RS02580 0.62 -1.0 580,571 - RR42_RS02580 0.62 +0.5 580,576 + RR42_RS02580 0.63 +0.4 580,577 - RR42_RS02580 0.63 -2.4 580,577 - RR42_RS02580 0.63 -0.2 580,583 + RR42_RS02580 0.64 +0.1 580,583 + RR42_RS02580 0.64 -0.7 580,714 + RR42_RS02580 0.82 +0.3 580,714 + RR42_RS02580 0.82 +1.2 580,715 - RR42_RS02580 0.82 +0.4 580,729 + RR42_RS02580 0.84 +1.5 580,759 + RR42_RS02580 0.88 +1.3 580,760 - RR42_RS02580 0.88 -1.3 580,760 - RR42_RS02580 0.88 +0.9 580,760 - RR42_RS02580 0.88 +0.3 580,843 + -0.5 580,843 + -0.5 580,843 + +0.3 580,844 - +1.4 580,844 - -0.1 580,844 - +0.3 580,863 + -1.1 580,863 + +0.3 580,863 + +1.3 580,863 + -0.7 580,864 - +1.0 580,864 - +0.0 580,864 - -1.1 580,864 - +0.4 580,864 - +0.0 580,865 + +1.7 580,865 + +0.3 580,865 + -0.3 580,865 + +1.0 580,865 + -0.3 580,865 + +0.1 580,866 - +0.2 580,866 - -0.7 580,866 - +1.8 580,866 - -0.6 580,866 - -1.3 580,866 - -0.4 580,866 - +0.3 580,866 - -0.0 581,037 + RR42_RS02585 0.26 -1.0 581,037 + RR42_RS02585 0.26 -0.0 581,037 + RR42_RS02585 0.26 -1.6 581,038 - RR42_RS02585 0.26 -1.1 581,038 - RR42_RS02585 0.26 +0.2
Or see this region's nucleotide sequence